1887

Abstract

Contradictory data have recently been reported on the role of the unique long–internal repeat junction area of pseudorabies (Aujeszky’s disease) virus (PrV) genome in the virulence of the virus. To investigate the basis of the difference, four recombinant PrVs mutated at the outer region of inverted repeats that involved a putative latency promoter (P) were constructed in this study. Propagation characteristics of mutant viruses in cultured cells were similar to those of the wild-type virus. However, a 757 bp deletion at this location caused significant reduction in the virulence of PrV after intraperitoneal inoculation of mice and a moderate decrease in the virulence after intracranial inoculation. These results indicate that the P region is an important virulence determinant that may be implicated in the neuroinvasive capability of the virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-2-415
2000-02-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/2/0810415a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-2-415&mimeType=html&fmt=ahah

References

  1. Banfield, B. W., Yap, G. S., Knapp, A. C. & Enquist, L. W. ( 1998; ). A chicken embryo eye model for the analysis of alphaherpesvirus neuronal spread and virulence. Journal of Virology 72, 4580-4588.
    [Google Scholar]
  2. Boldogköi, Z. & Murvai, J. ( 1994; ). A novel explanation for the existence of open reading frames on the latency-associated transcripts of alphaherpesviruses. Virus Genes 9, 47-51.[CrossRef]
    [Google Scholar]
  3. Boldogköi, Z., Murvai, J. & Fodor, I. ( 1995; ). G and C accumulation at silent positions of codons produces additional ORFs. Trends in Genetics 11, 125-126.
    [Google Scholar]
  4. Boldogköi, Z., Braun, A., Antal, J. & Fodor, I. ( 1998a; ). A restriction cleavage and transfection system for introducing foreign DNA sequences into the unique and inverted repeat regions of a herpesvirus. Research in Virology 149, 87-97.[CrossRef]
    [Google Scholar]
  5. Boldogköi, Z., Braun, A., Medveczky, I., Glávits, R., Gyúró, B. & Fodor, I. ( 1998b; ). Analysis of the equalization of inverted repeats and neurovirulence using a pseudorabies virus mutant strain altered at the Ul/Ir junction. Virus Genes 17, 89-98.[CrossRef]
    [Google Scholar]
  6. Card, J. P., Whealy, M. E., Robbins, A. K. & Enquist, L. W. ( 1992; ). Pseudorabies virus envelope glycoprotein gI influences both neurotropism and virulence during infection of the rat visual system. Journal of Virology 66, 3032-3041.
    [Google Scholar]
  7. Card, J. P., Enquist, L. W., Miller, A. D. & Yates, B. J. ( 1997; ). Differential tropism of pseudorabies virus for sensory neurons in the cat. Journal of Neurovirology 3, 49-61.[CrossRef]
    [Google Scholar]
  8. Cheung, A. K. ( 1991; ). Cloning of the latency gene and the early protein 0 gene of pseudorabies virus. Journal of Virology 65, 5260-5271.
    [Google Scholar]
  9. Dean, H. J. & Cheung, A. K. ( 1995; ). A deletion at the UL/IR junction reduces pseudorabies neurovirulence. Journal of Virology 69, 1334-1338.
    [Google Scholar]
  10. Dean, H. J., Miller, J. M., Ackerman, M. R., Gao, X. Y., Anderson, L. L., Jacobson, C. D. & Cheung, A. K. ( 1996; ). Replication and pathogenicity after intranasal and intracranial inoculation of swine with recombinant pseudorabies virus containing a deletion at the UL/IR junction. Virology 223, 19-28.[CrossRef]
    [Google Scholar]
  11. Elhai, J. & Wolk, C. P. ( 1988; ). A versatile class of positive-selection vectors based on the non-viability of palindrome containing plasmids, that allows cloning into the polylinkers. Gene 68, 119-138.[CrossRef]
    [Google Scholar]
  12. Field, H. J. & Hill, T. J. ( 1975; ). The pathogenesis of pseudorabies in mice: virus replication at the inoculation site and axonal uptake. Journal of General Virology 26, 145-148.[CrossRef]
    [Google Scholar]
  13. Kaplan, A. S. & Watter, A. E. ( 1959; ). A comparison of herpes simplex and pseudorabies virus. Virology 7, 394-407.[CrossRef]
    [Google Scholar]
  14. Lomniczi, B., Watanabe, S., Ben-Porat, T. & Kaplan, A. S. ( 1984; ). Genetic basis of neurovirulence of pseudorabies virus. Journal of Virology 52, 198-205.
    [Google Scholar]
  15. Rall, G. F., Kupershmidt, S., Sugg, N., Veach, R. A. & Ben-Porat, T. ( 1992; ). Functions of the sequences at the end of inverted repeats of pseudorabies virus. Journal of Virology 66, 1506-1519.
    [Google Scholar]
  16. Reed, L. J. & Muench, M. ( 1938; ). A simple method of estimating fifty percent end points. American Journal of Hygiene 27, 493-497.
    [Google Scholar]
  17. Ryan, P. & Shankly, F. L. ( 1996; ). A double-strand break in a herpesvirus genome stimulates targeted homologous recombination with exogenous, cloned viral sequences. Journal of Virological Methods 57, 95-107.[CrossRef]
    [Google Scholar]
  18. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  19. Vlcek, C., Kozmik, Z., Paces, V., Schirm, S. & Schwyzer, M. ( 1993; ). Pseudorabies virus immediate early gene overlaps with an oppositely oriented open reading frame – characterization of their promoter and enhancer regions. Virology 179, 365-377.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-2-415
Loading
/content/journal/jgv/10.1099/0022-1317-81-2-415
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error