1887

Journal of General Virology header logo

Volume 81, Issue 2

Mitochondrial distribution and function in herpes simplex virus-infected cells Free

Abstract

In this study, mitochondria migrated to a perinuclear region in the cytoplasm in herpes simplex virus (HSV)-infected cells. HSV infection did not promote the expression of cytochrome oxidase subunit 2 but did promote that of stress-responsive HSP60, both of which are known to be components of mitochondria. The levels of cellular ATP and lactate and mitochondrial membrane potential were maintained for at least 6 h but decreased at the late stage of infection. It was also found that the UL41 and UL46 gene products, both of which are known to be tegument proteins, accumulated in the perinuclear region. The clustering of mitochondria and the accumulation of tegument proteins were completely blocked by the addition of nocodazole and vinblastine. These results suggest that mitochondria respond to the stimulation of HSV infection, migrating with tegument proteins along microtubules to a site around the nucleus, and maintain function until at least the middle stage of infection.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-2-401
2000-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/2/0810401a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-2-401&mimeType=html&fmt=ahah

References

  1. Capaldi, R. A. (1990). Structure and function of cytochrome c oxidase.Annual Review of Biochemistry 59, 569-596.[CrossRef] [Google Scholar]
  2. Collier, N. C., Sheetz, M. P. & Schlesinger, M. J. (1993). Concomitant changes in mitochondria and intermediate filaments during heat shock and recovery of chicken embryo fibroblasts.Journal of Cellular Biochemistry 52, 297-307.[CrossRef] [Google Scholar]
  3. Daikoku, T., Yamashita, Y., Tsurumi, T., Maeno, K. & Nishiyama, Y. (1993). Purification and biochemical characterization of the protein kinase encoded by the US3 gene of herpes simplex virus type 2.Virology 197, 685-694.[CrossRef] [Google Scholar]
  4. Daikoku, T., Ikenoya, K., Yamada, H., Goshima, F. & Nishiyama, Y. (1998). Identification and characterization of the herpes simplex virus type 1 UL51 gene product.Journal of General Virology 79, 3027-3031. [Google Scholar]
  5. Dasgupta, A. & Wilson, D. W. (1999). ATP depletion blocks herpes simplex virus DNA packaging and capsid maturation.Journal of Virology 73, 2006-2015. [Google Scholar]
  6. Derfuss, T., Fickenscher, H., Kraft, M. S., Henning, G., Lengenfelder, D., Fleckenstein, B. & Meinl, E. (1998). Antiapoptotic activity of the herpesvirus saimiri-encoded Bcl-2 homolog: stabilization of mitochondria and inhibition of caspase-3-like activity.Journal of Virology 72, 5897-5904. [Google Scholar]
  7. Elliott, G. & O’Hare, P. (1999). Live-cell analysis of a green fluorescent protein-tagged herpes simplex virus infection.Journal of Virology 73, 4110-4119. [Google Scholar]
  8. Heggeness, M. H., Simon, M. & Singer, S. J. (1978). Association of mitochondria with microtubules in cultured cells.Proceedings of the National Academy of Sciences, USA 75, 3863-3866.[CrossRef] [Google Scholar]
  9. Johnson, L. V., Walsh, M. L., Bockus, B. J. & Chen, L. B. (1981). Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy.Journal of Cell Biology 88, 526-535.[CrossRef] [Google Scholar]
  10. Koyama, A. H., Akari, H., Adachi, A., Goshima, F. & Nishiyama, Y. (1998). Induction of apoptosis in HEp-2 cells by infection with herpes simplex virus type 2.Archives of Virology 143, 2435-2441.[CrossRef] [Google Scholar]
  11. Latchman, D. S. (1988). Effect of herpes simplex virus type 2 infection on mitochondrial gene expression.Journal of General Virology 69, 1405-1410.[CrossRef] [Google Scholar]
  12. La Thangue, N. B. & Latchman, D. S. (1988). A cellular protein related to heat-shock protein 90 accumulates during herpes simplex virus infection and is overexpressed in transformed cells.Experimental Cell Research 178, 169-179.[CrossRef] [Google Scholar]
  13. Lund, K. & Ziola, B. (1985). Cell sonicates used in the analysis of how measles and herpes simplex type 1 virus infections influence Vero cell mitochondrial calcium uptake.Canadian Journal of Biochemistry and Cell Biology 63, 1194-1197. [Google Scholar]
  14. Lund, K. & Ziola, B. (1986). Synthesis of mitochondrial macromolecules in herpes simplex type 1 virus infected Vero cells.Biochemistry and Cell Biology 64, 1303-1309.[CrossRef] [Google Scholar]
  15. Martin, C. S., Flores, A. I. & Cuezva, J. M. (1995). Cpn60 is exclusively localized into mitochondria of rat liver and embryonic Drosophila cells.Journal of Cell Biochemistry 59, 235-245.[CrossRef] [Google Scholar]
  16. Miller, R. H. & Lasek, R. J. (1985). Cross-bridges mediate anterograde and retrograde vesicle transport along microtubules in squid axoplasm.Journal of Cell Biology 101, 2181-2193.[CrossRef] [Google Scholar]
  17. Nakai, K. & Horton, P. (1999). PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization.Trends in Biochemical Sciences 24, 34-36.[CrossRef] [Google Scholar]
  18. Notarianni, E. L. & Preston, C. M. (1982). Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides.Virology 123, 113-122.[CrossRef] [Google Scholar]
  19. Rixon, F. J., Addison, C. & McLauchlan, J. (1992). Assembly of enveloped tegument structures (L particles) can occur independently of virion maturation in herpes simplex virus type 1-infected cells.Journal of General Virology 73, 277-284.[CrossRef] [Google Scholar]
  20. Roizman, B. (1996). The function of herpes simplex virus genes: a primer for genetic engineering of novel vectors.Proceedings of the National Academy of Sciences, USA 93, 11307-11312.[CrossRef] [Google Scholar]
  21. Rojo, G., Chamorro, M., Salas, M. L., Vinuela, E., Cuezva, J. M. & Salas, J. (1998). Migration of mitochondria to viral assembly sites in African swine fever virus-infected cells.Journal of Virology 72, 7583-7588. [Google Scholar]
  22. Shimizu, S., Eguchi, Y., Kamiike, W., Waguri, S., Uchiyama, Y., Matsuda, H. & Tsujimoto, Y. (1996). Bcl-2 blocks loss of mitochondrial membrane potential while ICE inhibitors act at a different step during inhibition of death induced by respiratory chain inhibitors.Oncogene 13, 21-29. [Google Scholar]
  23. Sodeik, B., Ebersold, M. W. & Helenius, A. (1997). Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus.Journal of Cell Biology 136, 1007-1021.[CrossRef] [Google Scholar]
  24. Tsurumi, T. & Lehman, I. R. (1990). Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection.Journal of Virology 64, 450-452. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-2-401
Loading
Mitochondrial distribution and function in herpes simplex virus-infected cells
J. Gen. Virol. 81, 401 (2000); https://doi.org/10.1099/0022-1317-81-2-401
/content/journal/jgv/10.1099/0022-1317-81-2-401
/content/journal/jgv/10.1099/0022-1317-81-2-401
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error