@article{mbs:/content/journal/jgv/10.1099/0022-1317-81-2-393, author = "Riegler, S. and Hebart, H. and Einsele, H. and Brossart, P. and Jahn, G. and Sinzger, C.", title = "Monocyte-derived dendritic cells are permissive to the complete replicative cycle of human cytomegalovirus", journal= "Journal of General Virology", year = "2000", volume = "81", number = "2", pages = "393-399", doi = "https://doi.org/10.1099/0022-1317-81-2-393", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-2-393", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The susceptibility of monocyte-derived immature dendritic cells (DC) to infection by various strains of human cytomegalovirus (HCMV) was analysed. Immature DC were generated by incubation of peripheral blood monocytes with interleukin-4 and granulocyte–macrophage colony-stimulating factor for 7 days and were characterized by a CD1a+/CD40+/CD80+/CD86+/HLA-DR+/CD14− phenotype. Viral antigen expression and production of infectious progeny virus were analysed in infected immature DC cultures. Immature DC were 80–90 % susceptible to HCMV strains that had been propagated in endothelial cell culture, whereas the infection rate was negligible with fibroblast-adapted HCMV strains. Immature DC infection resulted in expression of viral immediate early, early and late genes. Productive infection was proven by the detection of infectious virus in single-step growth curves and in infectious centre assays. It is concluded that HCMV might interfere with the host immune reaction by permissive, lytic infection of immature DC.", }