1887

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Volume 81, Issue 2

Monocyte-derived dendritic cells are permissive to the complete replicative cycle of human cytomegalovirus Free

Abstract

The susceptibility of monocyte-derived immature dendritic cells (DC) to infection by various strains of human cytomegalovirus (HCMV) was analysed. Immature DC were generated by incubation of peripheral blood monocytes with interleukin-4 and granulocyte–macrophage colony-stimulating factor for 7 days and were characterized by a CD1a+/CD40+/CD80+/CD86+/HLA-DR+/CD14− phenotype. Viral antigen expression and production of infectious progeny virus were analysed in infected immature DC cultures. Immature DC were 80–90 % susceptible to HCMV strains that had been propagated in endothelial cell culture, whereas the infection rate was negligible with fibroblast-adapted HCMV strains. Immature DC infection resulted in expression of viral immediate early, early and late genes. Productive infection was proven by the detection of infectious virus in single-step growth curves and in infectious centre assays. It is concluded that HCMV might interfere with the host immune reaction by permissive, lytic infection of immature DC.

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© Microbiology Society
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2000-02-01
2024-04-18
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Monocyte-derived dendritic cells are permissive to the complete replicative cycle of human cytomegalovirus
J. Gen. Virol. 81, 393 (2000); https://doi.org/10.1099/0022-1317-81-2-393
/content/journal/jgv/10.1099/0022-1317-81-2-393
/content/journal/jgv/10.1099/0022-1317-81-2-393
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