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Volume 81, Issue 2

BAG-1, a novel Bcl-2-interacting protein, activates expression of human JC virus Free

Abstract

Transcription of the human polyomavirus JC virus (JCV) genome is regulated by cellular proteins and the large tumour (T) antigen. Earlier studies led to the identification of nuclear factor-1 (NF-1)-binding sites in the JCV enhancer by DNase I protection assays of extracts from retinoic acid (RA)-differentiated P19 embryonal carcinoma (EC) cells. In this study, a cDNA clone that encodes a protein capable of binding to the JCV NF-1 sites was isolated from an RA-differentiated EC cell cDNA library. Sequence analysis revealed that the cDNA isolated was identical to the previously described Bcl-2-interacting protein BAG-1 (Bcl-2-associated athano gene-1). Results from RNA studies indicated that BAG-1 is expressed in several cell types. Co-transfection of a recombinant BAG-1 expression plasmid with JCV promoters indicated that BAG-1 stimulates transcription of the JCV promoter and to a lesser extent the JCV promoter. Mutations in the NF-1 sites in the JCV promoter eliminated the activation by BAG-1. Thus, BAG-1 is a novel transcription factor that may play a role in JCV expression.

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© Microbiology Society
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2000-02-01
2024-04-19
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References

  1. Ahmed, S., Chowdhury, M. & Khalili, K.(1990). Regulation of a human neurotropic virus promoter, JCVE: identification of a novel activator domain located upstream from the 98 bp enhancer promoter region.Nucleic Acids Research 18, 7417-7423.[CrossRef] [Google Scholar]
  2. Bardelli, A., Longati, P., Albero, D., Goruppi, S., Schneider, C., Ponzetto, C. & Comoglio, P. M.(1996). HGF receptor associates with the anti-apoptotic protein BAG-1 and prevents cell death.EMBO Journal 15, 6205-6212. [Google Scholar]
  3. Devireddy, L. R. (1995).Characterization of a cDNA encoding a novel transcription factor regulating expression of human neurotropic JC virus. MSc thesis, Memorial University of Newfoundland, St John’s, Newfoundland, Canada.
  4. Frisque, R. J., Bream, G. L. & Cannella, M. T.(1984). Human polyomavirus JC virus genome.Journal of Virology 51, 458-469. [Google Scholar]
  5. Froesch, B. A., Takayama, S. & Reed, J. C.(1998). BAG-1L protein enhances androgen receptor function.Journal of Biological Chemistry 273, 11660-11666.[CrossRef] [Google Scholar]
  6. Froesch, B. A., Aime-Sempe, C., Leber, B., Andrews, D. & Reed, J. C.(1999). Inhibition of p53 transcriptional activity by Bcl-2 requires its membrane-anchoring domain.Journal of Biological Chemistry 274, 6469-6475.[CrossRef] [Google Scholar]
  7. Henson, J. W., Saffer, J. & Furneaux, H.(1992). The transcription factor Sp1 binds to the JC virus promoter and is selectively expressed in glial cells in human brain.Annals of Neurology 32, 72-77.[CrossRef] [Google Scholar]
  8. Kenney, S., Natarajan, V., Strike, D., Khoury, G. & Salzman, N. P.(1984). JC virus enhancer-promoter active in human brain cells.Science 226, 1337-1339.[CrossRef] [Google Scholar]
  9. Khalili, K., Rappaport, J. & Khoury, G.(1988). Nuclear factors in human brain cells bind specifically to the JCV regulatory region.EMBO Journal 7, 1205-1210. [Google Scholar]
  10. Kullmann, M., Schneikert, J., Moll, J., Heck, S., Zeiner, M., Gehring, U. & Cato, A. C.(1998). RAP46 is a negative regulator of glucocorticoid receptor action and hormone-induced apoptosis.Journal of Biological Chemistry 273, 14620-14625.[CrossRef] [Google Scholar]
  11. Kumar, K. U. (1994).Transcriptional regulation of human JC virus. PhD thesis, Memorial University of Newfoundland, St John’s, Newfoundland, Canada.
  12. Kumar, K. U., Pater, A. & Pater, M. M.(1993). Human JC virus perfect palindromic nuclear factor 1-binding sequences important for glial cell-specific expression in differentiating embryonal carcinoma cells.Journal of Virology 67, 572-576. [Google Scholar]
  13. Liu, R., Takayama, S., Zheng, Y., Froesch, B., Chen, G. Q., Zhang, X., Reed, J. C. & Zhang, X. K.(1998). Interaction of BAG-1 with retinoic acid receptor and its inhibition of retinoic acid-induced apoptosis in cancer cells.Journal of Biological Chemistry 273, 16985-16992.[CrossRef] [Google Scholar]
  14. Major, E. O., Amemiya, K., Tornatore, C. S., Houff, S. A. & Berger, J. R. (1992). Pathogenesis and molecular biology of progressive multifocal leukoencephalopathy, the JC virus-induced demyelinating disease of the human brain.Clinical Microbiology Reviews 5, 49-73. [Google Scholar]
  15. Matsuzawa, S., Takayama, S., Froesch, B. A., Zapata, J. M. & Reed, J. C. (1998). p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1.EMBO Journal 17, 2736-2747.[CrossRef] [Google Scholar]
  16. Nakshatri, H., Pater, A. & Pater, M. M. (1990). Activity and enhancer binding factors for JC virus regulatory elements in differentiating embryonal carcinoma cells.Virology 177, 784-789.[CrossRef] [Google Scholar]
  17. Nakshatri, H., Pater, M. M. & Pater, A. (1991). Activity and enhancer binding factors for BK virus regulatory elements in differentiating embryonal carcinoma cells.Virology 183, 374-380.[CrossRef] [Google Scholar]
  18. Packham, G., Brimmell, M. & Cleveland, J. L. (1997). Mammalian cells express two differently localized Bag-1 isoforms generated by alternative translation initiation.Biochemical Journal 328, 807-813. [Google Scholar]
  19. Raj, G. V. & Khalili, K. (1995). Transcriptional regulation: lessons from the human neurotropic polyomavirus, JCV.Virology 213, 283-291.[CrossRef] [Google Scholar]
  20. Tada, H., Lashgari, M., Rappaport, J. & Khalili, K. (1989). Cell type-specific expression of JC virus early promoter is determined by positive and negative regulation.Journal of Virology 63, 463-466. [Google Scholar]
  21. Takayama, S., Sato, T., Krajewski, S., Kochel, K., Irie, S., Millan, J. A. & Reed, J. C. (1995). Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity.Cell 80, 279-284.[CrossRef] [Google Scholar]
  22. Takayama, S., Krajewski, S., Krajewska, M., Kitada, S., Zapata, J. M., Kochel, K., Knee, D., Scudiero, D., Tudor, G., Miller, G. J., Miyashita, T., Yamada, M. & Reed, J. C. (1998). Expression and location of Hsp70/Hsc-binding anti-apoptotic protein BAG-1 and its variants in normal tissues and tumor cell lines.Cancer Research 58, 3116-3131. [Google Scholar]
  23. Vinson, C. R., LaMarco, K. L., Johnson, P. F., Landschulz, W. H. & McKnight, S. L. (1988). In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.Genes & Development 2, 801-806.[CrossRef] [Google Scholar]
  24. Wang, H. G. & Reed, J. C. (1998). Bcl-2, Raf-1 and mitochondrial regulation of apoptosis.Biofactors 8, 13-16.[CrossRef] [Google Scholar]
  25. Wang, H. G., Takayama, S., Rapp, U. R. & Reed, J. C. (1996). Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1.Proceedings of the National Academy of Sciences, USA 93, 7063-7068.[CrossRef] [Google Scholar]
  26. Weber, T. & Major, E. O. (1997). Progressive multifocal leukoencephalopathy: molecular biology, pathogenesis and clinical impact.Intervirology 40, 98-111.[CrossRef] [Google Scholar]
  27. Wegner, M., Drolet, D. W. & Rosenfeld, M. G. (1993). Regulation of JC virus by the POU-domain transcription factor Tst-1: implications for progressive multifocal leukoencephalopathy.Proceedings of the National Academy of Sciences, USA 90, 4743-4747.[CrossRef] [Google Scholar]
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BAG-1, a novel Bcl-2-interacting protein, activates expression of human JC virus
J. Gen. Virol. 81, 351 (2000); https://doi.org/10.1099/0022-1317-81-2-351
/content/journal/jgv/10.1099/0022-1317-81-2-351
/content/journal/jgv/10.1099/0022-1317-81-2-351
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