%0 Journal Article %A van Duin, Mark %A Snijders, Peter J. F. %A Vossen, Mireille T. M. %A Klaassen, Erik %A Voorhorst, Feja %A Verheijen, René H. M. %A Helmerhorst, Theo J. %A Meijer, Chris J. L. M. %A Walboomers, Jan M. M. %T Analysis of human papillomavirus type 16 E6 variants in relation to p53 codon 72 polymorphism genotypes in cervical carcinogenesis %D 2000 %J Journal of General Virology, %V 81 %N 2 %P 317-325 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-81-2-317 %I Microbiology Society, %X This study aimed to assess the role of specific human papillomavirus type 16 (HPV-16) variants, in combination with p53 codon 72 polymorphism genotypes, in cervical carcinogenesis. An initial sequence analysis of HPV-16 long control, E6 and E7 regions of 53 well-defined cervical samples containing HPV-16 revealed that a T to G transition at nucleotide position 350 within the E6 open reading frame was the most common variation, the frequency of which seemed to decrease with increasing severity of the lesion. Therefore, a total of 246 cervical samples of residents of The Netherlands was specifically analysed for HPV-16 350G/T variants and/or p53 codon 72 genotypes. These comprised HPV-negative normal cervical scrapes (n=40), normal cervical scrapes containing HPV-16 (n=46), scrapes containing HPV-16 from women with abnormal cervical cytology participating in a non-intervention follow-up study without (n=38) and with (n=51) a histologically proven cervical intraepithelial neoplasia (CIN) III lesion at the end of the study, and cervical squamous cell carcinomas (n=71). Neither specific HPV-16 350G/T variants nor specific p53 genotypes were associated with a higher risk of developing CIN III or cervical cancer. However, HPV-16 350T variants were significantly over-represented in p53 Arg homozygous women with cervical cancer. This suggests that, in p53 Arg/Arg women, infection with HPV-16 350T variants confers a higher risk of cervical cancer. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-2-317