1887

Abstract

The putative replication initiation protein (Rep) of Coconut foliar decay virus (CFDV) was expressed as a 6× His recombinant protein in and in recombinant baculovirus. Purified 6× His–Rep protein was demonstrated to possess sequence non-specific RNA- and ssDNA-binding activities as well as magnesium-dependent ATPase/GTPase activity. The yeast two-hybrid system revealed that CFDV Rep could interact with itself. Subcellular distribution of the CFDV Rep was studied by fractionation of insect cells infected with recombinant baculovirus expressing the 6× His–Rep protein and by laser scanning confocal microscopy of epidermal cells bombarded with a construct encoding CFDV Rep fused to GFP. It was shown that CFDV Rep associated predominantly with nuclei and membranes of infected/transfected cells. These activities of CFDV-encoded Rep are very similar to those reported for Reps of geminiviruses.

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2000-12-01
2021-02-26
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