1887

Abstract

The braconid wasp carries polydnavirus (MdPDV) and parasitizes the larval stage of the moth injects MdPDV into larvae when it lays an egg and the virus infects various cells including haemocytes. Two new MdPDV transcripts expressed in host haemocytes were characterized in this study. Screening of an MdPDV-infected haemocyte cDNA library identified a 0·4 kb cDNA encoding a predicted protein of 103 amino acids which was named Egf0·4. This protein contained a cysteine-rich epidermal growth factor (EGF)-like motif at its N terminus that was similar to the EGF-like domains in the previously identified MdPDV genes · and ·. Sequencing of the genomic clone pMd-10 indicated that it contained the · gene, which consisted of two introns and three exons. This gene was located on MdPDV segment O and appeared to exist in multiple copies. A nucleic acid and expression screen identified a 1·8 kb cDNA encoding a predicted protein of 515 amino acids designated Glc1·8. This protein consisted of a heavily glycosylated central core of six tandemly arranged repeats flanked by hydrophobic N- and C-terminal domains. Northern blotting and hybridization studies indicated that both · and · were expressed in MdPDV-infected host haemocytes. Immunocytochemical studies also indicated that Glc1·8 localized to the cell surface.

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2000-12-01
2024-03-28
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References

  1. Ardman B., Sikorski M. A., Staunton D. E. 1992; CD43 interferes with T-lymphocyte adhesion. Proceedings of the National Academy of Sciences, USA 89:5001–5005
    [Google Scholar]
  2. Asgari S., Schmidt O., Theopold U. 1997; A polydnavirus-encoded protein of an endoparasitoid wasp is an immune suppressor. Journal of General Virology 78:3061–3070
    [Google Scholar]
  3. Davies D. H., Strand M. R., Vinson S. B. 1987; Changes in differential haemocyte count and in vitro behaviour of plasmatocytes from host Heliothis virescens caused by Campoletis sonorensis polydnavirus. Journal of Insect Physiology 33:143–153
    [Google Scholar]
  4. Devereux J. D., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  5. Edson K. M., Vinson S. B., Stoltz D. B., Summers M. D. 1981; Virus in a parasitoid wasp: suppression of the cellular immune response in the parasitoid’s host. Science 211:582–583
    [Google Scholar]
  6. Englund P. T. 1993; The structure and biosynthesis of glycosyl-phosphatidylinositol protein anchors. Annual Review of Biochemistry 62:121–138
    [Google Scholar]
  7. Gardiner E. M. M., Strand M. R. 1999; Monoclonal antibodies bind distinct classes of hemocytes in the moth Pseudoplusia includens. Journal of Insect Physiology 45:113–126
    [Google Scholar]
  8. Gupta R., Birch H., Rapacki K., Brunak S., Hansen J. E. 1999; O-Glycbase version 4.0: a revised database of O-glycosylated proteins. Nucleic Acids Research 27:370–372
    [Google Scholar]
  9. Guzo D., Stoltz D. B. 1985; Obligatory multiparasitism in the tussock moth, Orgyia leucostigma. Parasitology 90:1–10
    [Google Scholar]
  10. Guzo D., Stoltz D. B. 1987; Observations on cellular immunity and parasitism in the tussock moth. Journal of Insect Physiology 33:19–31
    [Google Scholar]
  11. Harlow E., Lane D. 1988 Antibodies: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  12. Hayes D. F., Silberstein D. S., Rodrique S. W., Kufe D. W. 1990; DF3 antigen, a human epithelial cell mucin, inhibits adhesion of eosinophils to antibody-coated targets. Journal of Immunology 145:962–970
    [Google Scholar]
  13. Hilkens J., Ligtenberg J. L., Vos H. L., Litvinov S. 1992; Cell membrane-associated mucins and their adhesion-modulating property. Trends in Biochemical Sciences 17:359–363
    [Google Scholar]
  14. Jiang H., Wang Y., Kanost M. R. 1998; Pro-phenol oxidase activating proteinase from an insect, Manduca sexta : a bacteria-inducible protein similar to Drosophila easter. Proceedings of the National Academy of Sciences, USA 95:12220–12225
    [Google Scholar]
  15. Lavine M. D., Beckage N. E. 1995; Temporal pattern of parasitism-induced immunosuppression in Manduca sexta larvae parasitized by Cotesia congregata. Journal of Insect Physiology 42:41–51
    [Google Scholar]
  16. Lee F.-J., Moss J., Lin L.-W. 1992; A simplified procedure for hybridization of RNA blots. Biotechniques 13:210–211
    [Google Scholar]
  17. Li X., Webb B. A. 1994; Apparent functional role for a cysteine-rich polydnavirus protein in suppression of the insect cellular immune response. Journal of Virology 68:7482–7489
    [Google Scholar]
  18. Ligtenberg J. J. L., Buijs F., Vos H. L., Hilkens J. 1992; Suppression of cellular aggregation by high levels of episialin. Cancer Research 52:2318–2324
    [Google Scholar]
  19. Pech L. L., Strand M. R. 1996; Granular cells are required for encapsulation of foreign targets by insect haemocytes. Journal of Cell Science 109:2053–2060
    [Google Scholar]
  20. Pringle J. H. W. 1938; Proprioception in insects. Journal of Experimental Biology 15:101–103
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  22. Shen Z., Dimopoulos G., Kafatos F. C., Jacobs-Lorena M. 1999; A cell surface mucin specifically expressed in the midgut of the malaria mosquito Anopheles gambiae. Proceedings of the National Academy of Sciences, USA 96:5610–5615
    [Google Scholar]
  23. Shepard M., Powell J. E., Jones W. Jr 1983; Biology of Microplitis demolitor (Hymenoptera: Braconidae), an imported parasitoid of Heliothis (Lepidoptera: Noctuidae) spp. and the soybean looper, Pseudoplusia includens (Lepidoptera: Noctuidae. Environmental Entomology 12:641–645
    [Google Scholar]
  24. Stanssens P., Bergum P. W., Gansemans Y., Jespers L., Laroche Y., Huang S., Maki S., Messens J., Lauwereys M., Cappello M., Hotez P. J., Lasters I., Vlasuk G. P. 1996; Anticoagulant repertoire of the hookworm Ancylostoma caninum. Proceedings of the National Academy of Sciences, USA 93:2149–2154
    [Google Scholar]
  25. Stoltz D. B. 1993; The polydnavirus life-cycle. In Parasites and Pathogens of Insects. , 1st edn. 1 pp 167–187 Edited by Beckage N. E., Federici B. A., Thompson S. N. San Diego, CA: Academic Press;
  26. Stoltz D. B., Guzo D. 1986; Apparent haemocytic transformations associated with parasitoid-induced inhibition of immunity in Malacosoma disstria larvae. Journal of Insect Physiology 32:377–388
    [Google Scholar]
  27. Strand M. R. 1990; Characterization of larval development in Pseudoplusia includens (Lepidoptera: Noctuidae. Annals of the Entomological Society of America 83:538–544
    [Google Scholar]
  28. Strand M. R. 1994; Microplitis demolitor polydnavirus infects and expresses in specific morphotypes of Pseudoplusia includens haemocytes. Journal of General of Virology 75:3007–3020
    [Google Scholar]
  29. Strand M. R., Johnson J. A. 1996; Characterization of monoclonal antibodies to hemocytes of Pseudoplusia includens. Journal of Insect Physiology 42:21–31
    [Google Scholar]
  30. Strand M. R., Noda T. 1991; Alterations in the haemocytes of Pseudoplusia includens after parasitism by Microplitis demolitor. Journal of Insect Physiology 37:839–850
    [Google Scholar]
  31. Strand M. R., Pech L. L. 1995a; Immunological basis for compatibility in parasitoid–host relationships. Annual Review of Entomology 40:31–56
    [Google Scholar]
  32. Strand M. R., Pech L. L. 1995b; Microplitis demolitor polydnavirus induces apoptosis of a specific haemocyte morphotype in Pseudoplusia includens. Journal of General Virology 76:283–291
    [Google Scholar]
  33. Strand M. R., Wong E. A. 1991; The growth and role of Microplitis demolitor teratocytes in parasitism of Pseudoplusia includens. Journal of Insect Physiology 37:503–515
    [Google Scholar]
  34. Strand M. R., Mckenzie D. I., Grassl V., Dover B. A., Aiken J. M. 1992; Persistence and expression of Microplitis demolitor polydnavirus in Pseudoplusia includens. Journal of General Virology 73:1627–1635
    [Google Scholar]
  35. Strand M. R., Witherell A., Trudeau D. 1997; Two Microplitis demolitor polydnavirus mRNAs expressed in hemocytes of Pseudoplusia includens contain a common cysteine-rich domain. Journal of Virology 71:2146–2156
    [Google Scholar]
  36. Strand M. R., Clark K. C., Gardiner E. M. M. 1999; Plasmatocyte spreading peptide does not induce Microplitis demolitor polydnavirus-infected plasmatocytes to spread on foreign surfaces. Archives of Insect Biochemistry and Physiology 40:41–52
    [Google Scholar]
  37. Tautz D., Pfeifle C. 1989; A non-radioactive in situ hybridization method for the localization of specific RNAs by Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98:81–85
    [Google Scholar]
  38. van de Wiel-Kemanade E., Ligtenberg M. J. L., de Boer A. J., Buijs F., Vos H. L., Melief C. J. M., Hilkens J., Fifdor C. G. 1993; Episialin (MUC1) inhibits cytotoxic lymphocyte–target cell interaction. Journal of Immunology 151:767–776
    [Google Scholar]
  39. van Klinken J.-W., Dekker J., Buller H. A., Einerhand A. W. C. 1995; Mucin gene structure and expression: protection vs adhesion. American Journal of Physiology 269:G613–G627
    [Google Scholar]
  40. von Heijne G. 1987 Sequence Analysis in Molecular Biology San Diego, CA: Academic Press;
  41. Webb B. A. 1998; Polydnavirus biology, genome structure, and evolution. In The Insect Viruses, 1st edn. pp 105–139 Edited by Miller L. K., Ball A. L. New York: Plenum;
    [Google Scholar]
  42. Wesseling J., van der Valk S. W., Vos H. L., Sonnenberg A., Hilkens J. 1995; Episialin (MUC1) overexpression inhibits integrin-mediated cell adhesion to extracellular matrix components. Journal of Cell Biology 129:255–265
    [Google Scholar]
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