RT Journal Article SR Electronic(1) A1 Wagner, Elke A1 Engelhardt, Othmar G. A1 Weber, Friedemann A1 Haller, Otto A1 Kochs, GeorgYR 2000 T1 Formation of virus-like particles from cloned cDNAs of Thogoto virus JF Journal of General Virology, VO 81 IS 12 SP 2849 OP 2853 DO https://doi.org/10.1099/0022-1317-81-12-2849 PB Microbiology Society, SN 1465-2099, AB Thogoto virus (THOV) is the type species of tick-transmitted orthomyxoviruses. Here, we describe the generation of virus-like particles (VLP) of THOV from cloned cDNAs. To synthesize the six structural proteins of THOV in mammalian cells, we used T7-controlled expression plasmids and a recombinant vaccinia virus producing T7 RNA polymerase. A minireplicon encoding a reporter gene flanked by THOV promoter sequences was expressed by the cellular RNA polymerase I. The recombinant proteins were functional in encapsidation, amplification and transcription of the minireplicon RNA. Furthermore, the artificial nucleocapsids were packaged into THO-VLPs that transferred the minireplicon to indicator cells. This system should be helpful in generating recombinant THOV entirely from cloned cDNAs., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-12-2849