1887

Abstract

In this study, four inhibitor of apoptosis genes () in the genome of nucleopolyhedrovirus (EppoMNPV) that are homologous to , , and genes of other baculoviruses have been identified. All four genes were sequenced and the and genes were shown to be functional inhibitors of apoptosis. The , and genes contain two baculovirus apoptosis inhibitor repeat motifs and a CHC RING finger-like motif. The activity of the genes was tested by transient expression in (Sf-21) cells treated with the apoptosis-inducing agents actinomycin D, cycloheximide, anisomycin, tumour necrosis factor-α and UV light. The gene prevented apoptosis induced by all agents tested, indicating activity towards a conserved component(s) of multiple apoptotic pathways. However, the gene was unable to function in the absence of a gene immediately upstream of that has homology to the gene of MNPV. The use of a CMV promoter rescued the apoptosis inhibition activity of the gene, indicating that the upstream homologue is associated with expression of . The gene was able to delay the onset of apoptosis caused by all of the induction agents tested but, unlike , was unable to prevent the development of an apoptotic response upon prolonged exposure of cells to the apoptosis induction agents. No anti-apoptotic activity was observed for the and genes of EppoMNPV.

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2000-11-01
2021-03-01
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