1887

Abstract

Persistence of adenovirus type 5 in blood has implications for the pathogenicity of the virus infection and for the use of this virus in oncolysis and gene therapy. In this study, the kinetics of adenovirus clearance from blood in mice has been evaluated. After a single inoculation of concentrated virus into the vena cava, virus half-life was less than 2 min. Depletion of Kupffer cells (KC) resulted in increased viraemia. After tail-vein injection, virus and latex beads co-localized within KC. An important factor in clearance by KC is the negative charge of particles. Deletion of the hexon hypervariable region 1 acidic stretch decreased the negative charge of the virion but it did not increase blood persistence. Coating with PEG (‘PEGylation’) reduced the clearance rate but also reduced infectivity.

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2000-11-01
2019-10-14
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References

  1. Alemany, R., Ruan, S., Kataoka, M., Koch, P. E., Mukhopadhyay, T., Cristiano, R. J., Roth, J. A. & Zhang, W. W. ( 1996; ). Growth inhibitory effect of anti-K-ras adenovirus on lung cancer cells. Cancer Gene Therapy 3, 296-301.
    [Google Scholar]
  2. Allemann, E., Brasseur, N., Benrezzak, O., Rousseau, J., Kudrevich, S. V., Boyle, R. W., Leroux, J. C., Gurny, R. & Van Lier, J. E. ( 1995; ). PEG-coated poly(lactic acid) nanoparticles for the delivery of hexadecafluoro zinc phthalocyanine to EMT-6 mouse mammary tumours. Journal of Pharmacy and Pharmacology 47, 382-387.[CrossRef]
    [Google Scholar]
  3. Brunner, K. T., Hurez, D., McCluskey, R. T. & Benacerraf, B. ( 1960; ). Blood clearance of P32-labeled vesicular stomatitis and Newcastle disease viruses by the reticuloendothelial system in mice. Journal of Immunology 85, 99-104.
    [Google Scholar]
  4. Chartier, C., Degryse, E., Gantzer, M., Dieterle, A., Pavirani, A. & Mehtali, M. ( 1996; ). Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli. Journal of Virology 70, 4805-4810.
    [Google Scholar]
  5. Chillon, M., Lee, J. H., Fasbender, A. & Welsh, M. J. ( 1998; ). Adenovirus complexed with polyethylene glycol and cationic lipid is shielded from neutralizing antibodies in vitro. Gene Therapy 5, 995-1002.[CrossRef]
    [Google Scholar]
  6. Crawford-Miksza, L. & Schnurr, D. P. ( 1996; ). Analysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues. Journal of Virology 70, 1836-1844.
    [Google Scholar]
  7. Davern, T. J., Sevcik, A. M. & Bissell, M. D. ( 1999; ). In vivo adenoviral vector-mediated gene delivery to non-parenchymal cells of the liver. Hepatology 30, 490A.
    [Google Scholar]
  8. Fechner, H., Haack, A., Wang, H., Wang, X., Eizema, K., Pauschinger, M., Schoemaker, R., Veghel, R., Houtsmuller, A., Schultheiss, H. P., Lamers, J. & Poller, W. ( 1999; ). Expression of coxsackie adenovirus receptor and αv-integrin does not correlate with adenovector targeting in vivo indicating anatomical vector barriers. Gene Therapy 6, 1520-1535.[CrossRef]
    [Google Scholar]
  9. Gabizon, A., Catane, R., Uziely, B., Kaufman, B., Safra, T., Cohen, R., Martin, F., Huang, A. & Barenholz, Y. ( 1994; ). Prolonged circulation time and enhanced accumulation in malignant exudates of doxorubicin encapsulated in polyethylene-glycol coated liposomes. Cancer Research 54, 987-992.
    [Google Scholar]
  10. Hierholzer, J. C. ( 1973; ). Further subgrouping of the human adenoviruses by differential hemagglutination. Journal of Infectious Diseases 128, 541-550.[CrossRef]
    [Google Scholar]
  11. Hitt, M. M., Addison, C. L. & Graham, F. L. ( 1997; ). Human adenovirus vectors for gene transfer into mammalian cells. Advances in Pharmacology 40, 137-206.
    [Google Scholar]
  12. Horwitz, M. S. ( 1996; ). Adenoviruses. In Fields Virology, pp. 2149-2171. Edited by B. N. Fields, D. N. Knipe & P. M. Howley. Philadelphia:Lippincott–Raven.
  13. Huang, S., Kamata, T., Takada, Y., Ruggeri, Z. M. & Nemerow, G. R. ( 1996; ). Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. Journal of Virology 70, 4502-4508.
    [Google Scholar]
  14. Jahrling, P. B. & Eddy, G. A. ( 1977; ). Comparisons among members of the Venezuelan encephalitis virus complex using hydroxylapatite column chromatography. American Journal of Epidemiology 106, 408-417.
    [Google Scholar]
  15. Kaner, R. J., Worgall, S., Leopold, P. L., Stolze, E., Milano, E., Hidaka, C., Ramalingam, R., Hackett, N. R., Singh, R., Bergelson, J., Finberg, R., Falck-Pedersen, E. & Crystal, R. G. ( 1999; ). Modification of the genetic program of human alveolar macrophages by adenovirus vectors in vitro is feasible but inefficient, limited in part by the low level of expression of the coxsackie/adenovirus receptor. American Journal of Respiratory Cell and Molecular Biology 20, 361-370.[CrossRef]
    [Google Scholar]
  16. Kirn, D. H. & McCormick, F. ( 1996; ). Replicating viruses as selective cancer therapeutics. Molecular Medicine Today 2, 519-527.[CrossRef]
    [Google Scholar]
  17. Kirn, A., Gut, J. P. & Gendrault, J. L. ( 1982; ). Interaction of viruses with sinusoidal cells. Progress in Liver Diseases 7, 377-392.
    [Google Scholar]
  18. Leopold, P. L., Ferris, B., Grinberg, I., Worgall, S., Hackett, N. R. & Crystal, R. G. ( 1998; ). Fluorescent virions: dynamic tracking of the pathway of adenoviral gene transfer vectors in living cells. Human Gene Therapy 9, 367-378.[CrossRef]
    [Google Scholar]
  19. Lieber, A., He, C. Y., Meuse, L., Schowalter, D., Kirillova, I., Winther, B. & Kay, M. A. ( 1997; ). The role of Kupffer cell activation and viral gene expression in early liver toxicity after infusion of recombinant adenovirus vectors. Journal of Virology 71, 8798-8807.
    [Google Scholar]
  20. Mei, Y. F. & Wadell, G. ( 1995; ). Molecular determinants of adenovirus tropism. Current Topics in Microbiology and Immunology 199, 213-228.
    [Google Scholar]
  21. Mittereder, N., March, K. L. & Trapnell, B. C. ( 1996; ). Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. Journal of Virology 70, 7498-7509.
    [Google Scholar]
  22. O’Riordan, C. R., Lachapelle, A., Delgado, C., Parkes, V., Wadsworth, S. C., Smith, A. E. & Francis, G. E. ( 1999; ). PEGylation of adenovirus with retention of infectivity and protection from neutralizing antibody in vitro and in vivo. Human Gene Therapy 10, 1349-1358.[CrossRef]
    [Google Scholar]
  23. Steffan, A. M. ( 1997; ). Hepatic macrophages and virus. Pathologie et Biologie 45, 169–183 (in French).
    [Google Scholar]
  24. Wolff, G., Worgall, S., van Rooijen, N., Song, W. R., Harvey, B. G. & Crystal, R. G. ( 1997; ). Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. Journal of Virology 71, 624-629.
    [Google Scholar]
  25. Worgall, S., Wolff, G., Falck-Pedersen, E. & Crystal, R. G. ( 1997; ). Innate immune mechanisms dominate elimination of adenoviral vectors following in vivo administration. Human Gene Therapy 8, 37-44.[CrossRef]
    [Google Scholar]
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