RT Journal Article SR Electronic(1) A1 Bembridge, Gary P. A1 Rodriguez, Nuria A1 Garcia-Beato, Regina A1 Nicolson, Carolyn A1 Melero, Jose A. A1 Taylor, GeraldineYR 2000 T1 DNA encoding the attachment (G) or fusion (F) protein of respiratory syncytial virus induces protection in the absence of pulmonary inflammation JF Journal of General Virology, VO 81 IS 10 SP 2519 OP 2523 DO https://doi.org/10.1099/0022-1317-81-10-2519 PB Microbiology Society, SN 1465-2099, AB Significant protection against respiratory syncytial virus (RSV) infection was induced in mice vaccinated intramuscularly (i.m.) with DNA encoding the F or G protein of RSV. The amounts of IgG1 of IgG2a antibodies in mice immunized with DNA-G alone were similar. However, the antibody response in mice co-immunized with DNA-G and DNA encoding IL-4 (DNA-IL-4) was strongly biased towards IgG1. In contrast, the antibody response in mice co-immunized with DNA-G and DNA-IL-2, -IL-12 or-IFN-γ was biased towards IgG2a. Mice vaccinated with DNA-F either alone or in combination with DNA encoding cytokines developed a predominant RSV-specific IgG2a response, which was most pronounced in mice co-immunized with DNA-F and DNA-IL-12 or -IFN-γ. Vaccinated mice developed only a slightly enhanced pulmonary inflammatory response following RSV challenge. More significantly, and in contrast to mice scarified with recombinant vaccinia virus expressing the G protein, mice vaccinated i.m. with DNA-G did not develop pulmonary eosinophilia, even when the immune response was biased towards a Th2 response by co-administration of DNA-IL-4., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-10-2519