1887

Abstract

Significant protection against respiratory syncytial virus (RSV) infection was induced in mice vaccinated intramuscularly (i.m.) with DNA encoding the F or G protein of RSV. The amounts of IgG1 of IgG2a antibodies in mice immunized with DNA-G alone were similar. However, the antibody response in mice co-immunized with DNA-G and DNA encoding IL-4 (DNA-IL-4) was strongly biased towards IgG1. In contrast, the antibody response in mice co-immunized with DNA-G and DNA-IL-2, -IL-12 or-IFN-γ was biased towards IgG2a. Mice vaccinated with DNA-F either alone or in combination with DNA encoding cytokines developed a predominant RSV-specific IgG2a response, which was most pronounced in mice co-immunized with DNA-F and DNA-IL-12 or -IFN-γ. Vaccinated mice developed only a slightly enhanced pulmonary inflammatory response following RSV challenge. More significantly, and in contrast to mice scarified with recombinant vaccinia virus expressing the G protein, mice vaccinated i.m. with DNA-G did not develop pulmonary eosinophilia, even when the immune response was biased towards a Th2 response by co-administration of DNA-IL-4.

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2000-10-01
2020-04-06
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References

  1. Alwan W. H., Openshaw P. J. M.. 1993; Distinct patterns of T- and B-cell immunity to respiratory syncytial virus induced by individual viral proteins. Vaccine11:431–437
    [Google Scholar]
  2. Alwan W. H., Record F. M., Openshaw P. J.. 1993; Phenotypic and functional characterization of T cell lines specific for individual respiratory syncytial virus proteins. Journal of Immunology150:5211–5218
    [Google Scholar]
  3. Bembridge G. P., Garcia-Beato R., Lopez J. A., Melero J. A., Taylor G.. 1998 a; Subcellular site of expression and route of vaccination influence pulmonary eosinophilia following respiratory syncytial virus challenge in BALB/c mice sensitized to the attachment G protein. Journal of Immunology161:2473–2480
    [Google Scholar]
  4. Bembridge G. P., Lopez J. A., Cook R., Melero J. A., Taylor G.. 1998 b; Recombinant vaccinia virus coexpressing the F protein of respiratory syncytial virus (RSV) and interleukin-4 (IL-4) does not inhibit the development of RSV-specific memory cytotoxic T lymphocytes, whereas priming is diminished in the presence of high levels of IL-2 or gamma interferon. Journal of Virology72:4080–4087
    [Google Scholar]
  5. Bembridge G. P., Lopez J. A., Bustos R., Melero J. A., Cook R., Mason H., Taylor G.. 1999; Priming with a secreted form of the fusion protein of respiratory syncytial virus (RSV) promotes interleukin-4 (IL-4) and IL-5 production but not pulmonary eosinophilia following RSV challenge. Journal of Virology73:10086–10094
    [Google Scholar]
  6. Connors M., Kulkarni A. B., Firestone C.-Y., Holmes K. L., Morse H. C.III., Sotnikov A. V., Murphy B. R.. 1992; Pulmonary histopathology induced by respiratory syncytial virus (RSV) challenge of formalin-inactivated RSV-immunized BALB/c mice is abrogated by depletion of CD4+ T cells. Journal of Virology66:7444–7451
    [Google Scholar]
  7. Connors M., Giese N. A., Kulkarni A. B., Firestone C. Y., Morse H. C.III., Murphy B. R.. 1994; Enhanced pulmonary histopathology induced by respiratory syncytial virus (RSV) challenge of formalin-inactivated RSV-immunized BALB/c mice is abrogated by depletion of interleukin-4 (IL-4) and IL-10. Journal of Virology68:5321–5325
    [Google Scholar]
  8. Donnelly J. J., Ulmer J. B., Shiver J. W., Liu M. A.. 1997; DNA vaccines. Annual Review of Immunology15:617–648
    [Google Scholar]
  9. Feltquate D. M., Heaney S., Webster R. G., Robinson H. L.. 1997; Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization. Journal of Immunology158:2278–2284
    [Google Scholar]
  10. Graham B. S., Henderson G. S., Tang Y. W., Lu X., Neuzil K. M., Colley D. G.. 1993; Priming immunization determines T helper cytokine mRNA expression patterns in lungs of mice challenged with respiratory syncytial virus. Journal of Immunology151:2032–2040
    [Google Scholar]
  11. Hussell T., Khan U., Openshaw P.. 1997; IL-12 treatment attenuates T helper cell type 2 and B cell responses but does not improve vaccine-enhanced lung illness. Journal of Immunology159:328–334
    [Google Scholar]
  12. Li X., Sambhara S., Li C. X., Ewasyshyn M., Parrington M., Caterini J., James O., Cates G., Du R.-P., Klein M.. 1998; Protection against respiratory syncytial virus infection by DNA immunization. Journal of Experimental Medicine188:681–688
    [Google Scholar]
  13. Lim Y. S., Kang B. Y., Kim E. J., Kim S. H., Hwang S. Y., Kim T. S.. 1998; Potentiation of antigen-specific, Th1 immune responses by multiple DNA vaccination with an ovalbumin/interferon-γ hybrid construct. Immunology94:135–141
    [Google Scholar]
  14. Openshaw P. J., Clarke S. L., Record F. M.. 1992; Pulmonary eosinophilic response to respiratory syncytial virus infection in mice sensitized to the major surface glycoprotein G. International Immunology4:493–500
    [Google Scholar]
  15. Pertmer T. M., Roberts T. R., Haynes J. R.. 1996; Influenza virus nucleoprotein-specific immunoglobulin G subclass and cytokine responses elicited by DNA vaccination are dependent on the route of vector DNA delivery. Journal of Virology70:6119–6125
    [Google Scholar]
  16. Schrijver R. S., Langedijk J. P. M., Keil G. M., Middel W. G. J., Maris-Veldhuis M., van Oirschot J. T., Rijsewijk F. A. M.. 1997; Immunization of cattle with a BHV1 vector vaccine or a DNA vaccine both coding for the G protein of BRSV. Vaccine15:1908–1916
    [Google Scholar]
  17. Seder R. A., Paul W. E.. 1994; Acquisition of lymphokine-producing phenotype by CD4+ T cells. Annual Review of Immunology12:635–673
    [Google Scholar]
  18. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K. K.-Y., King A. M. Q., Wertz G. W.. 1987; Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. Journal of Virology61:3855–3861
    [Google Scholar]
  19. Swain S. L., Weinberg A. D., English M., Huston G.. 1990; IL-4 directs the development of Th2-like helper effectors. Journal of Immunology145:3796–3806
    [Google Scholar]
  20. Tang Y. W., Graham B. S.. 1995; Interleukin-12 treatment during immunization elicits a T helper cell type 1-like immune response in mice challenged with respiratory syncytial virus and improves vaccine immunogenicity. Journal of Infectious Diseases172:734–738
    [Google Scholar]
  21. Taylor G., Stott E. J., Hughes M., Collins A. P.. 1984; Respiratory syncytial virus infection in mice. Infection and Immunity43:649–655
    [Google Scholar]
  22. Tripp R. A., Moore D., Jones L., Sullender W., Winter J., Anderson L. J.. 1999; Respiratory syncytial virus G and/or SH protein alters Th1 cytokines, natural killer cells, and neutrophils responding to pulmonary infection in BALB/c mice. Journal of Virology73:7099–7107
    [Google Scholar]
  23. Xiang Z., Ertl H. C.. 1995; Manipulation of the immune response to a plasmid-encoded viral antigen by coinoculation with plasmids expressing cytokines. Immunity2:129–135
    [Google Scholar]
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