1887

Abstract

The major envelope glycoprotein genes (ORF5) of seven Czech isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were amplified and their nucleotide sequences were determined. ORF5 displayed nucleotide and amino acid identities of 87·5–100% and 87·6–100%, respectively, among the isolates. In a phylogenetic tree, all European isolates were grouped in a genotype distinct from that of reference American strains (VR-2332, IAF-Klop). Among the European isolates, two different clades were identified. Two Czech isolates (V-501 and V-503) and Italian strain PRRSV 2156 fell into one clade. The remaining European strains comprised the second clade. Surprisingly, two separately clustered strains (V-501 and V-516) were isolated from the same herd. Additionally, the possible effect of cultivation on the nucleotide sequence was analysed. Nine point mutations in the ORF5 region resulted from 152 passages of the V-502 isolate in MARC-145 cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-10-2497
2000-10-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/10/0812497a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-10-2497&mimeType=html&fmt=ahah

References

  1. Andreyev, V. G., Wesley, R. D., Mengeling, W. L., Vorwald, A. C. & Lager, K. M. ( 1997; ). Genetic variation and phylogenetic relationships of 22 porcine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5. Archives of Virology 142, 993-1001.[CrossRef]
    [Google Scholar]
  2. Chen, Z., Li, K., Rowland, R. R. & Plagemann, P. G. ( 1998; ). Neuropathogenicity and susceptibility to immune response are interdependent properties of lactate dehydrogenase-elevating virus (LDV) and correlate with the number of N-linked polylactosaminoglycan chains on the ectodomain of the primary envelope glycoprotein. Advances in Experimental Medicine and Biology 440, 583-592.
    [Google Scholar]
  3. Dea, S., Gagnon, C. A., Mardassi, H. & Milane, G. ( 1996; ). Antigenic variability among North American and European strains of porcine reproductive and respiratory syndrome virus as defined by monoclonal antibodies to the matrix protein. Journal of Clinical Microbiology 34, 1488-1493.
    [Google Scholar]
  4. Drew, T. W., Meulenberg, J. J., Sands, J. J. & Paton, D. J. ( 1995; ). Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus. Journal of General Virology 76, 1361-1369.[CrossRef]
    [Google Scholar]
  5. Faaberg, K. S. & Plagemann, P. G. ( 1995; ). The envelope proteins of lactate dehydrogenase-elevating virus and their membrane topography. Virology 212, 512-525.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783-791.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. (1993). PHYLIP (Phylogeny Inference Package) version 3.5c. Department of Genetics, University of Washington, Seattle, WA. USA.
  8. Goldberg, T. L., Hahn, E. C., Weigel, R. M. & Scherba, G. ( 2000; ). Genetic, geographical and temporal variation of porcine reproductive and respiratory syndrome virus in Illinois. Journal of General Virology 81, 171-179.
    [Google Scholar]
  9. Kapur, V., Elam, M. R., Pawlovich, T. M. & Murtaugh, M. P. ( 1996; ). Genetic variation in porcine reproductive and respiratory syndrome virus isolates in the midwestern United States. Journal of General Virology 77, 1271-1276.[CrossRef]
    [Google Scholar]
  10. Keffaber, K. K. ( 1989; ). Reproductive failure of unknown etiology. American Association of Swine Practitioners Newsletter 1, 1-9.
    [Google Scholar]
  11. Kim, H. S., Kwang, J., Yoon, I. J., Joo, H. S. & Frey, M. L. ( 1993; ). Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Archives of Virology 133, 477-483.[CrossRef]
    [Google Scholar]
  12. Madsen, K. G., Hansen, C. M., Madsen, E. S., Strandbygaard, B., Botner, A. & Sorensen, K. J. ( 1998; ). Sequence analysis of porcine reproductive and respiratory syndrome virus of the American type collected from Danish swine herds. Archives of Virology 143, 1683-1700.[CrossRef]
    [Google Scholar]
  13. Mardassi, H., Mounir, S. & Dea, S. ( 1995; ). Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory syndrome virus, Quebec reference strain. Archives of Virology 140, 1405-1418.[CrossRef]
    [Google Scholar]
  14. Meng, X. J., Paul, P. S., Halbur, P. G. & Lum, M. A. ( 1995; ). Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in the USA and Europe. Archives of Virology 140, 745-755.[CrossRef]
    [Google Scholar]
  15. Meulenberg, J. J., Hulst, M. M., de Meijer, E. J., Moonen, P. L., den Besten, A., de Kluyver, E. P., Wensvoort, G. & Moormann, R. J. ( 1993; ). Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192, 62-72.[CrossRef]
    [Google Scholar]
  16. Meulenberg, J. J., Petersen-den Besten, A., de Kluyver, E. P., Moormann, R. J., Schaaper, W. M. & Wensvoort, G. ( 1995; ). Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology 206, 155-163.[CrossRef]
    [Google Scholar]
  17. Meulenberg, J. J., Petersen den Besten, A., de Kluyver, E., van Nieuwstadt, A., Wensvoort, G. & Moormann, R. J. ( 1997; ). Molecular characterization of Lelystad virus. Veterinary Microbiology 55, 197-202.[CrossRef]
    [Google Scholar]
  18. Murtaugh, M. P., Elam, M. R. & Kakach, L. T. ( 1995; ). Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Archives of Virology 140, 1451-1460.[CrossRef]
    [Google Scholar]
  19. Oleksiewicz, M. B., Botner, A., Madsen, K. G. & Storgaard, T. ( 1998; ). Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT–PCR amplification of whole viral genes. Veterinary Microbiology 64, 7-22.[CrossRef]
    [Google Scholar]
  20. Page, R. D. M. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357-358.
    [Google Scholar]
  21. Paton, D. J., Brown, I. H., Edwards, S. & Wensvoort, G. ( 1991; ). ‘Blue ear’ disease of pigs. Veterinary Record 128, 617.[CrossRef]
    [Google Scholar]
  22. Pirzadeh, B. & Dea, S. ( 1997; ). Monoclonal antibodies to the ORF5 product of porcine reproductive and respiratory syndrome virus define linear neutralizing determinants. Journal of General Virology 78, 1867-1873.
    [Google Scholar]
  23. Pirzadeh, B. & Dea, S. ( 1998; ). Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. Journal of General Virology 79, 989-999.
    [Google Scholar]
  24. Pirzadeh, B., Gagnon, C. A. & Dea, S. ( 1998; ). Genomic and antigenic variations of porcine reproductive and respiratory syndrome virus major envelope GP5 glycoprotein. Canadian Journal of Veterinary Research 62, 170-177.
    [Google Scholar]
  25. Plagemann, P. G. & Moennig, V. ( 1992; ). Lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Advances in Virus Research 41, 99-192.
    [Google Scholar]
  26. Suárez, P., Zardoya, R., Martin, M. J., Prieto, C., Dopazo, J., Solana, A. & Castro, J. M. ( 1996; ). Phylogenetic relationships of European strains of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and ORF-7 genes. Virus Research 42, 159-165.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.[CrossRef]
    [Google Scholar]
  28. Valı́ček, L., Pšikal, I., Šmı́d, B., Rodák, L., Kubalı́ková, R. & Kosinová, E. ( 1997; ). Isolation and identification of porcine reproductive and respiratory syndrome virus in cell cultures. Veterinarni Medicina 42, 281-287.
    [Google Scholar]
  29. Valı́ček, L., Pšikal, I., Šmı́d, B., Indik, S., Rodák, L. & Kosinová, E. ( 1999; ). Circulation of porcine reproductive and respiratory syndrome in swine herds in the Czech Republic. Veterinarni Medicina 44, 289-294.
    [Google Scholar]
  30. van Nieuwstadt, A. P., Meulenberg, J. J. M., van Essen-Zanbergen, A., Petersen-den Besten, A., Bende, R. J., Moormann, R. J. M. & Wensvoort, G. ( 1996; ). Proteins encoded by open reading frames 3 and 4 of the genome of Lelystad virus (Arteriviridae) are structural proteins of the virion. Journal of Virology 70, 4767-4772.
    [Google Scholar]
  31. Wensvoort, G., Terpstra, C., Pol, J. M. A., ter Laak, E. A., Bloemraad, M., de Kluyver, E. P., Kragten, C., van Buiten, L., den Besten, A., Wagenaar, F., Broekhuijsen, J. M., Moonen, P. L. J. M., Zetstra, T., de Boer, E. A., Tibben, H. J., de Jong, M. F., van’t Veld, P., Groenland, G. J. R., van Gennep, J. A., Voets, M. T., Verheijden, J. H. M. & Braamskamp, J. ( 1991; ). Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly 13, 121-130.[CrossRef]
    [Google Scholar]
  32. Wesley, R. D., Mengeling, W. L., Lager, K. M., Vorwald, A. C. & Roof, M. B. ( 1999; ). Evidence for divergence of restriction fragment length polymorphism patterns following in vivo replication of porcine reproductive and respiratory syndrome virus. American Journal of Veterinary Research 60, 463-467.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-10-2497
Loading
/content/journal/jgv/10.1099/0022-1317-81-10-2497
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error