Molecular characterization of the first Australian isolate of Japanese encephalitis virus, the FU strain Free

Abstract

The complete genomic and predicted amino acid sequence of the Japanese encephalitis virus (JEV) FU strain, a human isolate recovered from the first outbreak of Japanese encephalitis in Australian territory, was determined. Comparison of the FU genome with 15 fully sequenced JEV genomes revealed high levels of sequence identity, ranging from 88·7% (GP78) to 89·7% (K94P05) for nucleotides and 96·8% (K94P05) to 98·0% (JaGAr01) for amino acid sequences. A total of 39 unique amino acid differences were found in the FU strain polyprotein. Phylogenetic analyses were performed on all available full-length JEV genomes and a selection of 64 E gene sequences from temporally and geographically diverse JEV strains. For comparison with the E gene phylogeny, phylogenetic analysis using cognate prM gene sequences was also carried out. The FU strain was found to be most closely related to Korean isolate K94P05 in the full-length analysis and to Southeast Asian strains in the E and prM gene analyses. The E gene analysis corresponded well with the prM gene analysis and with previous genotyping studies using the prM gene. The epidemiological implications of this investigation are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-10-2471
2000-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/10/0812471a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-10-2471&mimeType=html&fmt=ahah

References

  1. Ali A., Igarashi A. 1997; Antigenic and genetic variations among Japanese encephalitis virus strains belonging to genotype 1. Microbiology and Immunology 41:241–252
    [Google Scholar]
  2. Burke D. S., Leake C. J. 1988; Japanese encephalitis. In Arboviruses: Epidemiology and Ecology pp 63–92 Edited by Monath T. P. Boca Raton: CRC Press;
    [Google Scholar]
  3. Chen W. R., Tesh R. B., Rico-Hesse R. 1990; Genetic variation of Japanese encephalitis virus in nature. Journal of General Virology 71:2915–2922
    [Google Scholar]
  4. Chen W. R., Rico-Hesse R., Tesh R. B. 1992; A new genotype of Japanese encephalitis virus from Indonesia. American Journal of Tropical Medicine and Hygiene 47:61–69
    [Google Scholar]
  5. Coia G., Parker M. D., Speight G., Byrne M. E., Westaway E. G. 1988; Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. Journal of General Virology 69:1–21
    [Google Scholar]
  6. Felsenstein J. 1993 PHYLIP: Phylogeny Inference Package, version 3.5c University of Washington; Seattle, WA, USA:
    [Google Scholar]
  7. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284
    [Google Scholar]
  8. Gould E. A., Clegg J. C. S. 1985; Growth, titration and purification of Togaviruses. In Virology: A Practical Approach pp 43–78 Edited by Mahy B. W. Oxford: IRL Press;
    [Google Scholar]
  9. Hanna J. N., Ritchie S. A., Phillips D. A., Shield J., Bailey M. C., Mackenzie J. S., Poidinger M., McCall B. J., Mills P. J. 1996; An outbreak of Japanese encephalitis in the Torres Strait. Australia; 1995 Medical Journal of Australia 165:256–260
    [Google Scholar]
  10. Hanna J. N., Ritchie S. A., Phillips D. A., Lee J. M., Hills S. L., van den Hurk A. F., Pyke A. T., Johansen C. A., Mackenzie J. S. 1999; Japanese encephalitis in north Queensland. Australia: 1998 Medical Journal of Australia 170:533–536
    [Google Scholar]
  11. Hasegawa H., Yoshida M., Fugita S., Kobayashi Y. 1994; Comparison of structural proteins among antigenically different Japanese encephalitis virus strains. Vaccine 12:841–844
    [Google Scholar]
  12. Hashimoto H., Nomoto A., Watanabe K., Mori T., Takezawa T., Aizawa C., Takegami T., Hiramatsu K. 1988; Molecular cloning and complete nucleotide sequence of the genome of Japanese encephalitis virus Beijing-1 strain. Virus Genes 1:305–317
    [Google Scholar]
  13. Huong V. T. Q., Ha D. Q., Deubel V. 1993; Genetic study of Japanese encephalitis viruses from Vietnam. American Journal of Tropical Medicine and Hygiene 49:538–544
    [Google Scholar]
  14. Jan L., Chen K., Lu C., Wu Y., Horng C. 1996; Complete nucleotide sequence of the genome of Japanese encephalitis virus Ling strain: the presence of a 25-nucleotide deletion in the 3′-nontranslated region. American Journal of Tropical Medicine and Hygiene 55:603–609
    [Google Scholar]
  15. Johansen C., Ritchie S., van den Hurk A., Bockarie M., Hanna J., Phillips D., Melrose W., Poidinger M., Scherret J., Hall R., Mackenzie J. 1997; The search for Japanese encephalitis virus in the Western province of Papua New Guinea. Arbovirus Research in Australia 7:131–136
    [Google Scholar]
  16. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120
    [Google Scholar]
  17. Kolaskar A. S., Kulkarni-Kale U. 1999; Prediction of three-dimensional structure and mapping of conformational epitopes of envelope glycoprotein of Japanese encephalitis virus. Virology 261:31–42
    [Google Scholar]
  18. Kuno G., Chang G. J. J., Tsuchiya K. R., Karabatsos N., Cropp C. B. 1998; Phylogeny of the genus Flavivirus. Journal of Virology 72:73–83
    [Google Scholar]
  19. Mackenzie J. S. 1999; The ecology of Japanese encephalitis virus in the Australasian region. Clinical Virology (Japan 27:1–17
    [Google Scholar]
  20. Mackenzie J. S., Poidinger M., Phillips D. A., Johansen C. A., Hall R. A., Hanna J. N., Ritchie S. A., Shield J., Graham R. 1997; Emergence of Japanese encephalitis virus in the Australasian region. In Factors in the Emergence of Arbovirus Diseases pp 191–201 Edited by Saluzzo J., Dodet B. Paris: Elsevier;
    [Google Scholar]
  21. McMinn P. C. 1997; The molecular basis of virulence of the encephalitogenic flaviviruses. Journal of General Virology 78:2711–2722
    [Google Scholar]
  22. Mangada M. N. M., Takegami T. 1999; Molecular characterization of the Japanese encephalitis virus representative immunotype strain JaGAr01. Virus Research 59:101–112
    [Google Scholar]
  23. Monath T. P., Heinz F. X. 1996; Flaviviruses. In Fields Virology pp 961–1034 Edited by Fields B., Knipe D., Howley P. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  24. Nam J. H., Chung Y. J., Ban S. J., Kim E. J., Park Y. K., Cho H. W. 1996; Envelope gene sequence variation among Japanese encephalitis viruses isolated in Korea. Acta Virologica 40:303–309
    [Google Scholar]
  25. Ni H., Barrett A. D. T. 1995; Nucleotide and deduced amino acid sequence of the structural protein genes of Japanese encephalitis viruses from different geographical locations. Journal of General Virology 76:401–407
    [Google Scholar]
  26. Nowak T., Wengler G. 1987; Analysis of disulphides present in the membrane proteins of West Nile flavivirus. Virology 156:127–137
    [Google Scholar]
  27. Okuno T., Okada T., Kondo A., Suzuki M., Kobayashi M., Oya A. 1968; Immunotyping of different strains of Japanese encephalitis virus by antibody-absorption, haemagglutination-inhibition and complement-fixation tests. Bulletin of the World Health Organization 38:547–563
    [Google Scholar]
  28. Olson J. G., Ksiazek T. G., Lee V. H., Tan R., Shope R. E. 1985; Isolation of Japanese encephalitis virus from Anopheles annularis and Anopheles vagus in Lombok, Indonesia. Transactions of the Royal Society of Tropical Medicine and Hygiene 79:845–847
    [Google Scholar]
  29. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12:357–358
    [Google Scholar]
  30. Paranjpe S., Banerjee K. 1996; Phylogenetic analysis of the envelope gene of Japanese encephalitis virus. Virus Research 42:107–117
    [Google Scholar]
  31. Poidinger M., Hall R. A., Mackenzie J. S. 1996; Molecular characterization of the Japanese encephalitis serocomplex of the flavivirus genus. Virology 218:417–421
    [Google Scholar]
  32. Rey F. A., Heinz F. X., Mandl C., Kunz C., Harrison S. C., Kuhn R. J., Rossmann M. G. 1995; The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolution. Nature 375:291–298
    [Google Scholar]
  33. Rice C. 1996; Flaviviridae : the viruses and their replication. In Fields Virology pp 931–959 Edited by Fields B., Knipe D., Howley P. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  34. Roehrig J. T., Hunt A. R., Johnson A. J., Hawkes R. A. 1989; Synthetic peptides derived from the deduced amino acid sequence of the E-glycoprotein of Murray Valley encephalitis virus elicit antiviral antibody. Virology 171:49–60
    [Google Scholar]
  35. Saitou U., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  36. Shield J., Hanna J., Phillips D. 1996; Reappearance of the Japanese encephalitis virus in the Torres Strait, 1996. Communicable Diseases Intelligence 20:191
    [Google Scholar]
  37. Spicer P. E., Phillips D. A., Pyke A. T., Johansen C. A., Melrose W., Hall R. A. 1999; Antibodies to Japanese encephalitis virus in human sera collected from Irian Jaya. Follow-up of a previously reported case of Japanese encephalitis in that region. Transactions of the Royal Society of Tropical Medicine and Hygiene 93:511–514
    [Google Scholar]
  38. Sumiyoshi H., Mori C., Fuke I., Morita K., Kuhara S., Kondou J., Kikuchi Y., Nagamatu H., Igarashi A. 1987; Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology 161:497–510
    [Google Scholar]
  39. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25:4876–4882
    [Google Scholar]
  40. Tsuchie H., Oda K., Vythilingam I., Thayan R., Vijayamalar B., Sinniah M., Singh J., Wada T., Tanaka H., Kurimura T., Igarashi A. 1997; Genotypes of Japanese encephalitis virus isolated in three states in Malaysia. American Journal of Tropical Medicine and Hygiene 56:153–158
    [Google Scholar]
  41. Vrati S., Giri R. K., Razdan A., Malik P. 1999; Complete nucleotide sequence of an Indian isolate of Japanese encephalitis virus: sequence comparison with other strains and phylogenetic analysis. American Journal of Tropical Medicine and Hygiene 61:677–680
    [Google Scholar]
  42. Westaway E. G., Blok J. 1997; Taxonomy and evolutionary relationships of flaviviruses. In Dengue and Dengue Haemorrhagic Fever pp 147–173 Edited by Gubler D. J., Kuno G. Wallingford: CAB International;
    [Google Scholar]
  43. Wu S., Lian W., Hsu L., Wu Y., Liau M. 1998; Antigenic characterization of nine wild-type Taiwanese isolates of Japanese encephalitis virus as compared with two vaccine strains. Virus Research 55:83–91
    [Google Scholar]
  44. Wuryadi S., Suroso T. 1989; Japanese encephalitis in Indonesia. Southeast Asian Journal of Tropical Medicine and Public Health 20:575–580
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-10-2471
Loading
/content/journal/jgv/10.1099/0022-1317-81-10-2471
Loading

Data & Media loading...

Most cited Most Cited RSS feed