1887

Abstract

Stem cuttings were collected in Cameroon from cassava plants displaying cassava mosaic disease (CMD) symptoms. The nature of the viruses present was determined by using the PCR with primers specific for the coat protein (CP) genes of African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV). All samples were infected by ACMV and eight of the 50 samples were infected by both ACMV and an EACMV-like virus. The complete nucleotide sequences of DNA-A and -B of representative ACMV and EACMV-like viruses were determined. The DNA-A component of the EACMV-like virus contained evidence of recombination in the AC2–AC3 region and DNA-B also contained evidence of recombination in BC1. However, both components retained gene arrangements typical of bipartite begomoviruses. When plants were doubly inoculated with these Cameroon isolates of ACMV and EACMV (ACMV/CM, EACMV/CM) by using sap from cassava plants or infectious clones, the symptoms were more severe than for plants inoculated with either virus alone. Southern blot analysis of viral DNAs from infected plants showed that there were significantly higher levels of accumulation of both ACMV/CM components and, to a lesser extent, of EACMV/CM components in mixed-infected plants than in singly infected plants. These results strongly suggest the occurrence of a synergistic interaction between the two viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-1-287
2000-01-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/1/0810287a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-1-287&mimeType=html&fmt=ahah

References

  1. Anjos, R. J., Jarfors, U. & Ghabrial, S. A. ( 1992; ). Soybean mosaic potyvirus enhances the titre of two comoviruses in dually infected soybean plants. Phytopathology 82, 1022-1027.[CrossRef]
    [Google Scholar]
  2. Berrie, L. C., Palmer, K. E., Rybicki, E. P. & Rey, M. E. C. ( 1998; ). Molecular characterisation of a distinct South African cassava infecting geminivirus. Archives of Virology 143, 2253-2260.[CrossRef]
    [Google Scholar]
  3. Briddon, R. W. & Markham, P. G. ( 1995; ). Family Geminiviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses, pp. 158-165. Edited by F. A. Murphy, C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M. A. Mayo & M. D. Summers. Vienna & New York: Springer-Verlag.
  4. Calvert, L. A. & Ghabrial, S. A. ( 1983; ). Enhancement by soybean mosaic virus of bean pod mottle virus titre in doubly infected soybean. Phytopathology 73, 992-997.[CrossRef]
    [Google Scholar]
  5. Damirdagh, I. S. & Ross, A. F. ( 1967; ). A marked synergistic interaction of potato viruses X and Y in inoculated leaves of tobacco. Virology 31, 296-307.[CrossRef]
    [Google Scholar]
  6. Dellaporta, S. L., Wood, J. & Hicks, J. B. ( 1983; ). A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1, 19-21.[CrossRef]
    [Google Scholar]
  7. Deng, D., Otim-Nape, W. G., Sangare, A., Ogwal, S., Beachy, R. N. & Fauquet, C. M. ( 1997; ). Presence of a new virus closely related to East African cassava mosaic geminivirus, associated with cassava mosaic outbreak in Uganda. African Journal of Root and Tuber Crops 2, 23-28.
    [Google Scholar]
  8. Fargette, D., Fauquet, C. & Thouvenel, J. C. ( 1988; ). Yield losses induced by African cassava mosaic virus in relation to the mode and date of infection. Tropical Pest Management 34, 89-91.[CrossRef]
    [Google Scholar]
  9. Fargette, D., Thresh, J. M. & Otim-Nape, G. W. ( 1994; ). The epidemiology of African cassava mosaic geminivirus: reversion and the concept of equilibrium. Tropical Science 34, 123-133.
    [Google Scholar]
  10. Fondong, V. N., Pita, J. S., Rey, C., Beachy, R. N. & Fauquet, C. M. ( 1998; ). First report of the presence of East African cassava mosaic virus in Cameroon. Plant Disease 82, 1172.
    [Google Scholar]
  11. Fondong, V. N., Thresh, J. M. & Fauquet, C. M. (2000). Field experiments in Cameroon on cassava mosaic virus disease and the reversion phenomenon in susceptible and resistant cassava cultivars. International Journal of Pest Management (in press).
  12. Gilbertson, R. L., Faria, J. C., Hanson, S. F., Morales, F. J., Ahlquist, P., Maxwell, D. P. & Russell, D. R. ( 1991; ). Cloning of complete DNA genomes of four bean-infecting geminiviruses and determining their infectivity by electric discharge particle acceleration. Phytopathology 81, 980-985.[CrossRef]
    [Google Scholar]
  13. Goldberg, K. B. & Brakke, M. K. ( 1987; ). Concentration of maize chlorotic mosaic virus increased in mixed infections with maize dwarf mosaic virus strain B. Phytopathology 77, 162-167.[CrossRef]
    [Google Scholar]
  14. Harrison, B. D., Zhou, X., Otim-Nape, G. W., Liu, Y. & Robinson, D. J. ( 1997; ). Role of a novel type of double infection in the geminivirus-induced epidemic of severe cassava mosaic in Uganda. Annals of Applied Biology 131, 437-448.[CrossRef]
    [Google Scholar]
  15. Hong, Y. G., Robinson, D. J. & Harrison, B. D. ( 1993; ). Nucleotide sequence evidence for the occurrence of three distinct whitefly-transmitted geminiviruses in cassava. Journal of General Virology 74, 2437-2443.[CrossRef]
    [Google Scholar]
  16. Morris, B., Coates, L., Lowe, S., Richardson, K. & Eddy, P. ( 1990; ). Nucleotide sequence of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain). Nucleic Acids Research 18, 197-198.[CrossRef]
    [Google Scholar]
  17. Padidam, M., Beachy, R. N. & Fauquet, C. M. ( 1995; ). Classification and identification of geminiviruses using sequence comparisons. Journal of General Virology 76, 249-263.[CrossRef]
    [Google Scholar]
  18. Padidam, M., Sawyer, S. & Fauquet, C. M. (1999). Possible emergence of new geminiviruses by frequent recombination. Virology (in press).
  19. Pita, J. S., Fondong, V. N., Sangare, A., Otim-Nape, G. W., Ogwal, S. & Fauquet, C. M. (1999). Biodiversity of cassava mosaic disease in East and West Africa: special cases in Uganda and Ivory Coast. In Proceedings of the 4th International Scientific Meeting of the Cassava Biotechnology Network. Salvador, Bahia, Brazil. November 3–7, 1999 (in press).
  20. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  21. Stanley, J. & Gray, M. R. ( 1983; ). Nucleotide sequence of cassava latent virus DNA. Nature 301, 260-262.[CrossRef]
    [Google Scholar]
  22. Stanley, J., Markham, P. G., Callis, R. J. & Pinner, M. S. ( 1986; ). The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus. EMBO Journal 5, 1761-1767.
    [Google Scholar]
  23. Swanson, M. M. & Harrison, B. D. ( 1994; ). Properties, relationships and distribution of cassava mosaic geminiviruses. Tropical Science 34, 15-25.
    [Google Scholar]
  24. Umaharan, P., Padidam, M., Phelps, R. H., Beachy, R. N. & Fauquet, C. M. ( 1998; ). Distribution and diversity of geminiviruses in Trinidad and Tobago. Phytopathology 88, 1262-1268.[CrossRef]
    [Google Scholar]
  25. Vance, V. B. ( 1991; ). Replication of potato virus X RNA is altered in coinfections with potato virus Y. Virology 182, 486-494.[CrossRef]
    [Google Scholar]
  26. Vance, V. B., Berger, P. H., Carrington, J. C., Hunt, A. G. & Shi, X. M. ( 1995; ). 5′ proximal potyviral sequences mediate potato virus X/potyviral synergistic disease in transgenic tobacco. Virology 206, 583-590.[CrossRef]
    [Google Scholar]
  27. von Arnim, A. & Stanley, J. ( 1992; ). Determinants of tomato golden mosaic virus symptom development located on DNA B. Virology 186, 286-293.[CrossRef]
    [Google Scholar]
  28. Zhou, X., Liu, Y., Calvert, L., Munoz, C., Otim-Nape, G. W., Robinson, D. J. & Harrison, B. D. ( 1997; ). Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. Journal of General Virology 78, 2101-2111.
    [Google Scholar]
  29. Zhou, X., Liu, Y., Robinson, D. J. & Harrison, B. D. ( 1998a; ). Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra. Journal of General Virology 79, 915-923.
    [Google Scholar]
  30. Zhou, X., Robinson, D. J. & Harrison, B. D. ( 1998b; ). Types of variation in DNA-A among isolates of East African cassava mosaic virus from Kenya, Malawi and Tanzania. Journal of General Virology 79, 2835-2840.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-1-287
Loading
/content/journal/jgv/10.1099/0022-1317-81-1-287
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error