1887

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Volume 81, Issue 1

Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Free

Abstract

cDNA copies of the coat protein (CP) gene of (IPCV)-H were introduced into cells of or by transformation with vectors based on pROKII or pET respectively. In both plant and bacterial cells, IPCV CP was expressed and assembled to form virus-like particles (VLP). In plant extracts, the smallest preponderant particle length was about 50 nm. Other abundant lengths were about 85 and about 120 nm. The commonest VLP length in bacterial extracts was about 30 nm. Many of the longer VLP appeared to comprise aggregates of shorter particles. The lengths of the supposed ‘monomer’ VLP corresponded approximately to those expected for encapsidated CP gene transcript RNA. Immunocapture RT–PCR, using primers designed to amplify the CP gene, confirmed that the VLP contained RNA encoding IPCV-H CP. The results show that encapsidation does not require the presence of the 5′-terminal untranslated sequence of the virus RNA and suggest that if there is an ‘origin of assembly’ motif or sequence, it lies within the CP gene. When transgenic plants expressing IPCV-H CP were inoculated with IPCV-L, a strain that is serologically distinct from IPCV-H, the virus particles that accumulated contained both types of CP.

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2000-01-01
2024-04-20
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References

  1. Barker, H., Reavy, B., McGeachy, K. D. & Dawson, S. (1998). Transformation of Nicotiana benthamiana with the potato mop-top virus coat protein gene produces a novel resistance phenotype mediated by the coat protein. Molecular Plant–Microbe Interactions 11, 626-633.[CrossRef] [Google Scholar]
  2. Brandt, S. & Himmler, G. (1995). Detection of nepoviruses in ligneous grapevine material by using IC/PCR. Vitis 34, 127-128. [Google Scholar]
  3. Butler, P. J. G. & Mayo, M. A. (1987). Molecular architecture and assembly of tobacco mosaic virus particles. In The Molecular Biology of the Positive Strand RNA Viruses, pp. 237-257. Edited by D. J. Rowlands, M. A. Mayo & B. W. J. Mahy. London: Academic Press.
  4. Gilmer, D., Bouzoubaa, S., Hehn, A., Guilley, H., Richards, K. E. & Jonard, G. (1992). Efficient cell-to-cell movement of BNYVV requires 3′ proximal genes located on RNA-2. Virology 189, 40-47.[CrossRef] [Google Scholar]
  5. Goulden, M. G., Davies, J. W., Wood, K. R. & Lomonossoff, G. P. (1992). Structure of tobraviral particles: a model suggested from sequence conservation in tobraviral and tobamoviral coat proteins. Journal of Molecular Biology 227, 1-8.[CrossRef] [Google Scholar]
  6. Guilley, H., Jonard, G., Richards, K. E. & Hirth, L. (1975). Sequence of a specifically encapsidated RNA fragment originating from the tobacco mosaic virus coat protein cistron. European Journal of Biochemistry 54, 135-144.[CrossRef] [Google Scholar]
  7. Hammond, J. & Dienelt, M. M. (1997). Encapsidation of potyviral RNA in various forms of transgene coat protein is not correlated with resistance in transgenic plants.Molecular Plant–Microbe Interactions 10, 1023-1027.[CrossRef] [Google Scholar]
  8. Hirth, L. & Richards, K. E. (1981). Tobacco mosaic virus. Advances in Virus Research 26, 145-199. [Google Scholar]
  9. Hwang, D.-J., Roberts, I. M. & Wilson, T. M. A. (1994). Expression of tobacco mosaic virus coat protein and assembly of pseudovirus particles in Escherichia coli.Proceedings of the National Academy of Sciences, USA 91, 9067-9071.[CrossRef] [Google Scholar]
  10. Jacquet, C., Delecolle, B., Raccah, B., Lecoq, H., Dunez, J. & Ravelonandro, M. (1998). Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants. Journal of General Virology 79, 1509-1517. [Google Scholar]
  11. Jagadish, M. N., Ward, C. W., Gough, K. H., Tulloch, P. A., Wittaker, L. A. & Shukla, D. D. (1991). Expression of potyvirus coat protein in Escherichia coli and yeast and its assembly into virus-like particles. Journal of General Virology 72, 1543-1550.[CrossRef] [Google Scholar]
  12. Jagadish, M. N., Huang, D. & Ward, C. W. (1993). Site-directed mutagenesis of a potyvirus coat protein and its assembly in Escherichia coli. Journal of General Virology 74, 893-896.[CrossRef] [Google Scholar]
  13. Mason, H. S., Ball, J. M., Shi, J.-J., Jiang, X., Estes, M. K. & Arntzen, C. J. (1996). Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proceedings of the National Academy of Sciences, USA 93, 5335-5340.[CrossRef] [Google Scholar]
  14. Mayo, M. A. & Reddy, D. V. R. (1985). Translation products of RNA from Indian peanut clump virus. Journal of General Virology 66, 1347-1352.[CrossRef] [Google Scholar]
  15. Mayo, M. A. & Naidu, R. A. (1997). Peanut clump disease: an overview. In Groundnut Virus Diseases in Africa: Summary and Recommendations of the Sixth Meeting of the International Working Group, pp. 22-23. Edited by D. V. R. Reddy, P. Delfosse, J. M. Lenné & P. Subrahmanyam. Patancheru, Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics.
  16. Mayo, M. A., Brierley, K. M. & Goodman, B. A. (1993). Developments in the understanding of the particle structure of tobraviruses. Biochimie 75, 639-644.[CrossRef] [Google Scholar]
  17. Miller, J. S., Wesley, S. V., Naidu, R. A., Reddy, D. V. R. & Mayo, M. A. (1996). The nucleotide sequence of RNA-1 of Indian peanut clump furovirus. Archives of Virology 141, 2301-2312.[CrossRef] [Google Scholar]
  18. Nolt, B. L., Rajeshwari, R., Reddy, D. V. R., Bharathan, N. & Manohar, S. K. (1988). Indian peanut clump virus isolates: host range, symptomatology, serological relationships and some physical properties. Phytopathology 78, 310-313.[CrossRef] [Google Scholar]
  19. Reddy, D. V. R., Rajeshwari, R., Iizuka, W., Lesemann, D. E., Nolt, B. L. & Goto, T. (1983). The occurrence of Indian peanut clump, a soil-borne virus disease of groundnuts (Arachis hypogaea) in India. Annals of Applied Biology 102, 305-310.[CrossRef] [Google Scholar]
  20. Reddy, D. V. R., Robinson, D. J., Roberts, I. M. & Harrison, B. D. (1985). Genome properties and relationships of Indian peanut clump virus. Journal of General Virology 66, 2011-2016.[CrossRef] [Google Scholar]
  21. Reddy, D. V. R., Nolt, B. L., Hobbs, H. A., Reddy, A. S., Rajeshwari, R., Rao, A. S., Reddy, D. D. R. & McDonald, D. (1988). Clump virus in India: isolates, host range, transmission and management. In Developments in Applied Biology II. Viruses with Fungal Vectors, pp. 239-246. Edited by J. I. Cooper & M. J. C. Asher. Wellesbourne, UK: Association of Applied Biologists.
  22. Roberts, I. M. (1986). Immunoelectron microscopy of extracts of virus-infected plants. In Electron Microscopy of Proteins, vol. 5, Viral Topics, pp. 293-357. Edited by J. R. Harris & R. W. Horne. New York: Academic Press.
  23. Rochow, W. F. (1977). Dependent virus transmission from mixed infections. In Aphids as Virus Vectors, pp. 253-273. Edited by K. F. Harris & K. Maramorosch. New York: Academic Press.
  24. Sleat, D. F., Gallie, D. R., Watts, J. W., Deom, C. M., Turner, P. C., Beachy, R. N. & Wilson, T. M. A. (1989). Selective recovery of target gene transcripts in virus-like particles of TMV-infected transgenic tobacco. Nucleic Acids Research 16, 3127-3140. [Google Scholar]
  25. Stram, Y., Sela, I., Edelbaum, O., Tanne, E., Karchi, M. & Karchi, H. (1993). Expression and assembly of the potato virus Y (PVY) coat protein (CP) in Escherichia coli cells.Virus Research 28, 29-35.[CrossRef] [Google Scholar]
  26. Wellink, J., Verver, J., Van Lent, L. & Van Kammen, A. (1996). Capsid proteins of cowpea mosaic virus transiently expressed in protoplasts form virus-like particles. Virology 224, 352-355.[CrossRef] [Google Scholar]
  27. Wesley, S. V. (1993).Isolation and utilization of gene sequences from Indian peanut clump virus RNA towards diagnosis and control of clump disease of groundnut in India. PhD thesis, Department of Agricultural Engineering, Indian Institute of Technology, Kharagpur, India.
  28. Wesley, S. V., Mayo, M. A., Jolly, C. A., Naidu, R. A., Reddy, D. V. R., Jana, M. K. & Parnaik, V. K. (1994). The coat protein of Indian peanut clump virus: relationship amongst furoviruses and barley stripe mosaic virus. Archives of Virology 134, 271-278.[CrossRef] [Google Scholar]
  29. Yusibov, V., Kumar, A., North, A., Johnson, J. E. & Loesch-Fries, L. S. (1996). Purification, characterization, assembly and crystallization of assembled alfalfa mosaic virus coat protein expressed in Escherichia coli. Journal of General Virology 77, 567-573.[CrossRef] [Google Scholar]
  30. Zhao, X., Fox, J. M., Olson, N. H., Baker, T. S. & Young, M. J. (1995).In vitro assembly of cowpea chlorotic mottle virus from coat protein expressed in Escherichia coli and in vitro transcribed viral cDNA. Virology 207, 486-494.[CrossRef] [Google Scholar]
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Virus-like particles assemble in plants and bacteria expressing the coat protein gene of Indian peanut clump virus
J. Gen. Virol. 81, 267 (2000); https://doi.org/10.1099/0022-1317-81-1-267
/content/journal/jgv/10.1099/0022-1317-81-1-267
/content/journal/jgv/10.1099/0022-1317-81-1-267
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