%0 Journal Article %A van der Meulen, Karen M. %A Nauwynck, Hans J. %A Buddaert, Wim %A Pensaert, Maurice B. %T Replication of equine herpesvirus type 1 in freshly isolated equine peripheral blood mononuclear cells and changes in susceptibility following mitogen stimulation %D 2000 %J Journal of General Virology, %V 81 %N 1 %P 21-25 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-81-1-21 %I Microbiology Society, %X In the present study, the outcome of an inoculation of equine peripheral blood mononuclear cells (PBMC) with equine herpesvirus type 1 (EHV-1) was studied in vitro. Cytoplasmic and plasma membrane expression of viral antigens, intra- and extracellular virus titres, and plaque formation in co-culture were determined. EHV-1 replicated in monocytes, although in a highly restricted way. Viral antigens were found at maximum levels (8·7% of the monocytes) at 12 h post-infection. The infection was productive in 0·16% of the monocytes. The virus yield was 100·7 TCID50 per productive cell. In a population of resting lymphocytes, 0·9% of cells were infected and less than 0·05% produced infectious virus. After prestimulation with different mitogens, the number of infected lymphocytes increased four to twelve times. The susceptible lymphocytes were T-lymphocytes. In mitogen-stimulated lymphocytes, clear expression of viral antigens was found on the plasma membrane. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-1-21