The ability of variants of the human respiratory syncytial virus (HRSV) phosphoprotein (P protein) to support RNA transcription and replication has been studied by using HRSV-based subgenomic replicons. The serine residues normally phosphorylated in P during HRSV infection have been replaced by other residues. The results indicate that the bulk of phosphorylation of P (98%) is not essential for viral RNA transcription or replication but that phosphorylation can modulate these processes.


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  1. Barik, S., McLean, T. & Dupuy, L. C. (1995). Phosphorylation of Ser232 directly regulates the transcriptional activity of the P protein of human respiratory syncytial virus: phosphorylation of Ser237 may play an accessory role. Virology 213, 405-412.[CrossRef] [Google Scholar]
  2. Collins, P. L., McIntosh, K. & Chanock, R. M. (1995). Respiratory syncytial virus. In Fields Virology, pp. 1313-1351. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  3. Collins, P. L., Hill, M. G., Cristina, J. & Grosfeld, H. (1996). Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus. Proceedings of the National Academy of Sciences, USA 93, 81-85.[CrossRef] [Google Scholar]
  4. Fuerst, T. R., Earl, P. L. & Moss, B. (1987). Use of a hybrid vaccinia virus–T7 RNA polymerase system for expression of target genes. Molecular and Cellular Biology 7, 2538-2544. [Google Scholar]
  5. Garcı́a, J., Garcı́a-Barreno, B., Vivo, A. & Melero, J. A. (1993). Cytoplasmic inclusions of respiratory syncytial virus-infected cells: formation of inclusion bodies in transfected cells that coexpress the nucleoprotein, the phosphoprotein, and the 22K protein. Virology 195, 243-247.[CrossRef] [Google Scholar]
  6. Hardy, R. W. & Wertz, G. W. (1998). The product of the respiratory syncytial virus M2 gene ORF1 enhances readthrough of intergenic junctions during viral transcription. Journal of Virology 72, 520-526. [Google Scholar]
  7. Huang, Y. T., Collins, P. L. & Wertz, G. W. (1985). Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. Virus Research 2, 157-173.[CrossRef] [Google Scholar]
  8. Lambert, D. M., Hambor, J., Diebold, M. & Galinski, B. (1988). Kinetics of synthesis and phosphorylation of respiratory syncytial virus polypeptides. Journal of General Virology 69, 313-323.[CrossRef] [Google Scholar]
  9. López, J. A., Villanueva, N., Melero, J. A. & Portela, A. (1988). Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence of subtype genetic heterogeneity. Virus Research 10, 249-261.[CrossRef] [Google Scholar]
  10. Mazumder, B. & Barik, S. (1994). Requirement of casein kinase II-mediated phosphorylation for the transcriptional activity of human respiratory syncytial viral phosphoprotein P: transdominant negative phenotype of phosphorylation-defective P mutants. Virology 205, 104-111.[CrossRef] [Google Scholar]
  11. Mazumder, B., Adhikary, G. & Barik, S. (1994). Bacterial expression of human respiratory syncytial viral phosphoprotein P and identification of Ser237 as the site of phosphorylation by cellular casein kinase II. Virology 205, 93-103.[CrossRef] [Google Scholar]
  12. Navarro, J., López-Otı́n, C. & Villanueva, N. (1991). Location of phosphorylated residues in human respiratory syncytial virus phosphoprotein. Journal of General Virology 72, 1455-1459.[CrossRef] [Google Scholar]
  13. Roach, P. J. (1991). Multisite and hierarchal protein phosphorylation. Journal of Biological Chemistry 266, 14139-14142. [Google Scholar]
  14. Sánchez-Seco, M. P., Navarro, J., Martı́nez, R. & Villanueva, N. (1995). C-Terminal phosphorylation of human respiratory syncytial virus P protein occurs mainly at serine residue 232. Journal of General Virology 76, 425-430.[CrossRef] [Google Scholar]
  15. Villanueva, N., Navarro, J. & Cubero, E. (1991). Antiviral effects of xanthate D609 on the human respiratory syncytial virus growth cycle. Virology 181, 101-108.[CrossRef] [Google Scholar]
  16. Villanueva, N., Navarro, J., Méndez, E. & Garcı́a-Albert, I. (1994). Identification of a protein kinase involved in the phosphorylation of the C-terminal region of human respiratory syncytial virus P protein. Journal of General Virology 75, 555-565.[CrossRef] [Google Scholar]
  17. Yu, Q., Hardy, R. W. & Wertz, G. W. (1995). Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, and L proteins support replication of RS virus genomic RNA analogs and define minimal trans-acting requirements for RNA replication. Journal of Virology 69, 2412-2419. [Google Scholar]

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