1887

Abstract

Brome mosaic virus (BMV) purified from systemically infected barley leaves 8 weeks post-inoculation (p.i.) contained defective RNAs (D-RNAs). The D-RNAs were detected in total and virion RNAs extracted from infected plants at 8 weeks p.i. or later, but not before, when barley plants had been inoculated with virions either containing or lacking D-RNA. The D-RNAs were derived from genomic RNA3 by double or mainly single deletions in the 3a protein ORF, and formed a heterogeneous population. By using transcripts of D-RNA synthesized from full-length cDNA clones, the D-RNAs were shown to replicate in a helper virus-dependent manner and to be packaged into virions in barley protoplasts. Subgenomic RNA4 was produced from the D-RNA and the coat protein was also expressed. Existence of the D-RNAs together with BMV genomic RNAs in inoculated protoplasts decreased the accumulation of 3a protein but it had no apparent effect on the accumulation of BMV genomic RNA3 or the coat protein. This is the first report of naturally occurring D-RNAs generated during prolonged infection with BMV.

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1999-09-01
2024-03-29
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References

  1. Ahlquist P. 1992; Bromovirus RNA replication and transcription. Current Opinion in Genetics and Development 2:71–76
    [Google Scholar]
  2. Ahlquist P., French R., Janda M., Loesch-Fries L. S. 1984; Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proceedings of the National Academy of Sciences USA 81:7066–7070
    [Google Scholar]
  3. Bujarski J. J., Dzianott A. M. 1991; Generation and analysis of nonhomologous RNA–RNA recombinants in brome mosaic virus: sequence complementarities at crossover sites. Journal of Virology 65:4153–4159
    [Google Scholar]
  4. Bujarski J. J., Nagy P. D., Flasinski S. 1994; Molecular studies of genetic RNA–RNA recombination in brome mosaic virus. Advances in Virus Research 43:275–302
    [Google Scholar]
  5. Burgyan J., Grieco F., Russo M. 1989; A defective interfering RNA molecule in cymbidium ringspot virus infections. Journal of General Virology 70:235–239
    [Google Scholar]
  6. French R., Janda M., Ahlquist P. 1986; Bacterial gene inserted in an engineered RNA virus; efficient expression in monocotyledonous plant cells. Science 231:1294–1297
    [Google Scholar]
  7. Fujita Y., Mise K., Okuno T., Ahlquist P., Furusawa I. 1996; A single codon change in a conserved motif of a bromovirus movement protein gene confers compatibility with a new host. Virology 223:283–291
    [Google Scholar]
  8. Fujita M., Mise K., Kajiura Y., Dohi K., Furusawa I. 1998; Nucleic acid-binding properties and subcellular localization of the 3a protein of brome mosaic bromovirus. Journal of General Virology 79:1273–1280
    [Google Scholar]
  9. Graves M. V., Roossinck M. J. 1995; Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus. Journal of Virology 69:4746–4751
    [Google Scholar]
  10. Hillman B. I., Carrington J. C., Morris T. J. 1987; A defective interfering RNA that contains a mosaic of a plant virus genome. Cell 51:427–433
    [Google Scholar]
  11. Holland J. J. 1990; Defective viral genome. In Virology pp 151–165 Edited by Fields B. N., Knibe D. M. New York: Raven Press;
    [Google Scholar]
  12. Huang A. S. 1977; Viral pathogenesis and molecular biology. Bacteriological Reviews 41:811–821
    [Google Scholar]
  13. Kaido M., Mori M., Mise K., Okuno T., Furusawa I. 1995; Inhibition of brome mosaic virus (BMV) amplification in protoplasts from transgenic tobacco plants expressing replicable BMV RNAs. Journal of General Virology 76:2827–2833
    [Google Scholar]
  14. Kibertis P. A., Loesch-Fries L. C., Hall T. C. 1981; Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  15. Knorr D. A., Mullin R. H., Hearne P. Q., Morris T. J. 1991; De novo generation of defective interfering RNAs of tomato bushy stunt virus by high multiplicity passage. Virology 181:193–202
    [Google Scholar]
  16. Kroner P., Ahlquist P. 1992; RNA-based viruses. In Molecular Plant Pathology: A Practical Approach pp 23–34 Edited by Gurr S. J., McPherson M. J., Bowles D. J. Oxford: Oxford University Press;
    [Google Scholar]
  17. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154:367–382
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  19. Lane L. C. 1981; Bromoviruses. In Handbook of Plant Virus Infections: Comparative Diagnosis pp 333–376 Edited by Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  20. Levis R., Weiss B. G., Tsiang M., Huang H., Schlesinger S. 1986; Deletion mapping of Sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging. Cell 44:137–145
    [Google Scholar]
  21. Li X. H., Heaton L. A., Morris T. J., Simon A. E. 1989; Turnip crinkle virus defective interfering RNAs intensify viral symptoms and are generated de novo. Proceedings of the National Academy of Sciences USA 869173–9177
    [Google Scholar]
  22. Marsh L. E., Pogue G. P., Connell J. P., Hall T. C. 1991; Artificial defective interfering RNAs derived from brome mosaic virus. Journal of General Virology 72:1787–1792
    [Google Scholar]
  23. Miller W. A., Dreher T. W., Hall T. C. 1985; Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (−)-sense genomic RNA. Nature 313:68–70
    [Google Scholar]
  24. Mise K., Tsuge S., Nagao K., Okuno T., Furusawa I. 1992; Nucleotide sequence responsible for the synthesis of a truncated coat protein of brome mosaic virus strain ATCC66. Journal of General Virology 73:2543–2551
    [Google Scholar]
  25. Mise K., Mori M., Nakayashiki H., Koyama T., Okuno T., Furusawa I. 1994; Nucleotide sequence of a set of cDNA clones derived from brome mosaic virus ATCC66 strain and comparison with the Russian strain genome. Annals of the Phytopathological Society of Japan 60:454–462
    [Google Scholar]
  26. Mori M., Mise K., Kobayashi K., Okuno T., Furusawa I. 1991; Infectivity of plasmids containing brome mosaic virus cDNA linked to the cauliflower mosaic virus 35 S RNA promoter. Journal of General Virology 72:243–246
    [Google Scholar]
  27. Mori M., Kaido M., Okuno T., Furusawa I. 1993; mRNA amplification system by viral replicase in transgenic plants. FEBS Letters 336:171–174
    [Google Scholar]
  28. Morris T. J., Hillman B. I. 1989; Defective interfering RNAs of a plant virus. In Molecular Biology of Plant–Pathogen Interactions pp 185–197 Edited by Staskawitcz B., Ahlquist P., Yoder O. New York: Alan R. Liss;
    [Google Scholar]
  29. Nagano H., Okuno T., Mise K., Furusawa I. 1997; Deletion of the C-terminal 33 amino acids of cucumber mosaic virus movement protein enables a chimeric brome mosaic virus to move from cell to cell. Journal of Virology 71:2270–2276
    [Google Scholar]
  30. Okuno T., Furusawa I. 1978; Modes of infection of barley protoplasts with brome mosaic virus. Journal of General Virology 38:409–418
    [Google Scholar]
  31. Okuno T., Furusawa I. 1979; RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts. Virology 99:218–225
    [Google Scholar]
  32. Perrault J. 1981; Origin and replication of defective interfering particles. Current Topics in Microbiology and Immunology 93:151–207
    [Google Scholar]
  33. Pogany J., Romero J., Huang Q., Sgro J.-Y., Shang H., Bujarski J. J. 1995; De novo generation of defective interfering-like RNAs in broad bean mottle bromovirus. Virology 212:574–586
    [Google Scholar]
  34. Rochon D. M. 1991; Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein. Proceedings of the National Academy of Sciences USA 88:11153–11157
    [Google Scholar]
  35. Rochon D. M., Johnston J. C. 1991; Infectious transcripts from cloned cucumber necrosis virus cDNA: evidence for a bifunctional subgenomic mRNA. Virology 181:656–665
    [Google Scholar]
  36. Romero J., Huang Q., Pogany J., Bujarski J. J. 1993; Characterization of defective interfering RNA components that increase symptom severity of broad bean mottle virus infections. Virology 194:576–584
    [Google Scholar]
  37. Roux L., Simon A. E., Holland J. J. 1991; Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Advances in Virus Research 40:181–211
    [Google Scholar]
  38. Schlesinger S. 1988; The generation and amplification of defective interfering RNAs. In RNA Genetics, Retroviruses, Viroids and RNA Recombination pp 167–185 Edited by Domingo E., Holland J. J., Ahlquist P. Boca Raton, FL: CRC Press;
    [Google Scholar]
  39. Schmitz I., Rao A. L. N. 1996; Molecular studies on bromovirus capsid protein. I. Characterization of cell-to-cell movement-defective RNA3 variants of brome mosaic virus. Virology 226:281–293
    [Google Scholar]
  40. Steinhauer D. A., Holland J. J. 1987; Rapid evolution of RNA viruses. Annual Review of Microbiology 41:409–433
    [Google Scholar]
  41. Vieira J., Messing J. 1987; Production of single-stranded plasmid DNA. Methods in Enzymology 153:3–11
    [Google Scholar]
  42. Weiss B., Nitschko H., Ghattas I., Wright R., Schlesinger S. 1989; Evidence for specificity in the encapsidation of Sindbis virus RNAs. Journal of Virology 63:5310–5318
    [Google Scholar]
  43. White K. A., Bancroft J. B., Mackie G. A. 1991; Defective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products. Virology 183:479–486
    [Google Scholar]
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