1887

Abstract

A pair of degenerate PCR primers (FAP59/64) was designed from two relatively conserved regions of the L1 open reading frame of most human papillomaviruses (HPV). The size of the generated amplicon was about 480 bp. PCR using these primers was found capable of amplifying DNA from 87% (65/75) of the HPV types tested, its sensitivity being 1–10 copies for HPV-5, -20 and -30 clones. HPV was found in 63% (5/8) of tumour samples and in 63% (5/8) of normal skin biopsies from patients with various cutaneous tumours. HPV-5, HPV-8, HPV-12, HPVvs20-4 and six putatively novel HPV types were identified. No correlation was found to exist between specific HPV and tumour types. Skin surface swab samples from one or more sites on three of four healthy volunteers were found to contain HPV, types 12 and 49 being identified, as well as eight novel HPV types, two of which were also found among the patients. In all, HPV was detected in 75% (9/12) of those tested, five HPV types and 12 novel candidate types being identified, and 37% (7/19) of HPV-positive samples were found to manifest more than one HPV type. All the HPV detected manifested high degrees of nucleotide sequence similarity with HPV types associated with skin lesions and epidermodysplasia verruciformis. The overall HPV finding in the skin samples was 50% (20/40) using the FAP primers as compared to 18% (7/40) using another PCR test designed for skin types. The results thus suggest the new method to be sensitive and generally applicable for detecting cutaneous HPV.

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1999-09-01
2019-10-23
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References

  1. Bens, G., Wieland, U., Hofmann, A., Höpfl, R. & Pfister, H. ( 1998; ). Detection of new human papillomavirus sequences in skin lesions of a renal transplant recipient and characterization of one complete genome related to epidermodysplasia verruciformis-associated types. Journal of General Virology 79, 779-787.
    [Google Scholar]
  2. Berkhout, R. J., Tieben, L. M., Smits, H. L., Bavinck, J. N., Vermeer, B. J. & ter Schegget, J. ( 1995; ). Nested PCR approach for detection and typing of epidermodysplasia verruciformis-associated human papillomavirus types in cutaneous cancers from renal transplant recipients. Journal of Clinical Microbiology 33, 690-695.
    [Google Scholar]
  3. Boxman, I. L., Berkhout, R. J., Mulder, L. H., Wolkers, M. C., Bouwes Bavinck, J. N., Vermeer, B. J. & ter Schegget, J. ( 1997; ). Detection of human papillomavirus DNA in plucked hairs from renal transplant recipients and healthy volunteers. Journal of Investigative Dermatology 108, 712-715.[CrossRef]
    [Google Scholar]
  4. de Jong-Tieben, L. M., Berkhout, R. J., Smits, H. L., Bouwes Bavinck, J. N., Vermeer, B. J., van der Woude, F. J. & ter Schegget, J. ( 1995; ). High frequency of detection of epidermodysplasia verruciformis-associated human papillomavirus DNA in biopsies from malignant and premalignant skin lesions from renal transplant recipients. Journal of Investigative Dermatology 105, 367-371.[CrossRef]
    [Google Scholar]
  5. de Roda Husman, A.-M., Walboomers, J. M. M., van den Brule, A. J. C., Meijer, C. J. L. M. & Snijders, P. J. F. ( 1995; ). The use of general primers GP5 and GP6 elongated at their 3′ ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. Journal of General Virology 76, 1057-1062.[CrossRef]
    [Google Scholar]
  6. de Villiers, E. M., Lavergne, D., McLaren, K. & Benton, E. C. ( 1997; ). Prevailing papillomavirus types in non-melanoma carcinomas of the skin in renal allograft recipients. International Journal of Cancer 73, 356-361.[CrossRef]
    [Google Scholar]
  7. Guerrero, E. & Shah, K. V. ( 1991; ). Polymerase chain reaction in HPV diagnosis. Papillomavirus Report 2, 115-118.
    [Google Scholar]
  8. Hopfl, R., Bens, G., Wieland, U., Petter, A., Zelger, B., Fritsch, P. & Pfister, H. ( 1997; ). Human papillomavirus DNA in non-melanoma skin cancers of a renal transplant recipient: detection of a new sequence related to epidermodysplasia verruciformis associated types. Journal of Investigative Dermatology 108, 53-56.[CrossRef]
    [Google Scholar]
  9. Jablonska, S. & Majewski, S. ( 1994; ). Epidermodysplasia verruciformis: immunological and clinical aspects. Current Topics in Microbiology and Immunology 186, 157-175.
    [Google Scholar]
  10. Manos, M. M., Ting, Y., Wright, D. K., Lewis, A. J., Broker, T. R. & Wolinsky, S. M. ( 1989; ). The use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells 7, 209-214.
    [Google Scholar]
  11. Myers, G. (1996). Alignments. In Human Papillomaviruses 1996. HPV Sequence Database, pp. II-L1–1–67. Edited by G. Myers, C. Baker, K. Münger, F. Sverdrup, A. McBride & H. U. Bernard. Los Alamos.
  12. Myers, G. (1997). Alignments. In Human Papillomaviruses 1997. HPV Sequence Database, pp. II-L1–23–73. Edited by G. Myers, C. Baker, K. Münger, F. Sverdrup, A. McBride & H. U. Bernard. Los Alamos.
  13. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Shamanin, V., Delius, H. & de Villiers, E.-M. ( 1994a; ). Development of a broad spectrum PCR assay for papillomaviruses and its application in screening lung cancer biopsies. Journal of General Virology 75, 1149-1156.[CrossRef]
    [Google Scholar]
  15. Shamanin, V., Glover, M., Rausch, C., Proby, C., Leigh, I. M., zur Hausen, H. & de Villiers, E. M. ( 1994b; ). Specific types of human papillomavirus found in benign proliferations and carcinomas of the skin in immunosuppressed patients. Cancer Research 54, 4610-4613.
    [Google Scholar]
  16. Shamanin, V., zur Hausen, H., Lavergne, D., Proby, C. M., Leigh, I. M., Neumann, C., Hamm, H., Goos, M., Haustein, U. F., Jung, E. G., Plewig, G., Wolff, H. & de Villiers, E.-M. ( 1996; ). Human papillomavirus infections in nonmelanoma skin cancers from renal transplant recipients and nonimmunosuppressed patients. Journal of the National Cancer Institute 88, 802-811.[CrossRef]
    [Google Scholar]
  17. Smits, H. L., Tieben, L. M., Tjong-A-Hung, S. P., Jebbink, M. F., Minnaar, R. P., Jansen, C. L. & ter Schegget, J. ( 1992; ). Detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types. Journal of General Virology 73, 3263-3268.[CrossRef]
    [Google Scholar]
  18. Surentheran, T., Harwood, C. A., Spink, P. J., Sinclair, A. L., Leigh, I. M., Proby, C. M., McGregor, J. M. & Breuer, J. ( 1998; ). Detection and typing of human papillomaviruses in mucosal and cutaneous biopsies from immunosuppressed and immunocompetent patients and patients with epidermodysplasia verruciformis: a unified diagnostic approach. Journal of Clinical Pathology 51, 606-610.[CrossRef]
    [Google Scholar]
  19. zur Hausen, H. ( 1996; ). Papillomavirus infections – a major cause of human cancers. Biochimica et Biophysica Acta 1288, F55-78.
    [Google Scholar]
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