1887

Abstract

The UL14 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 219 amino acid protein with a molecular mass of 23 kDa. In this study, the HSV-2 UL14 gene product has been identified by using a rabbit polyclonal antiserum raised against a recombinant 6×His–UL14 fusion protein expressed in . The antiserum reacted specifically with 34, 33 and 28 kDa proteins in HSV-2-infected cell lysates and also with a 34 kDa protein produced by transcription and translation reactions, suggesting that the 34 kDa protein is the primary translation product of the UL14 gene. The protein was synthesized at late times post-infection (p.i.) and was not detectable in the presence of the viral DNA synthesis inhibitor acycloguanosine. Indirect immunofluorescence studies localized the UL14 protein both to the nucleus and to perinuclear regions of the cytoplasm, and the nuclear UL14 protein was found to co-localize with the scaffolding protein ICP35 at 9 h p.i. However, the protein accumulated in a perinuclear region of the cytoplasm at 12 h p.i., while most of the ICP35 protein localized within assemblons in the nucleus. Although no detectable UL14 protein was associated with intracellular capsids isolated in the presence of 0·5 M NaCl, it was detected in purified virions. Furthermore, the UL14 protein expressed alone was detected both in the nucleus and in the cytoplasm at 24 h after transfection, but was mainly localized to the cytoplasm at later times.

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1999-09-01
2019-10-19
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References

  1. Addison, C., Rixon, F. J. & Preston, V. G. ( 1990; ). Herpes simplex virus type 1 UL28 gene product is important for the formation of mature capsids. Journal of General Virology 71, 2377-2384.[CrossRef]
    [Google Scholar]
  2. Ali, M. A., Forghani, B. & Cantin, E. M. ( 1996; ). Characterization of an essential HSV-1 protein encoded by the UL25 gene reported to be involved in virus penetration and capsid assembly. Virology 216, 278-283.[CrossRef]
    [Google Scholar]
  3. al-Kobaisi, M. F., Rixon, F. J., McDougall, I. & Preston, V. G. ( 1991; ). The herpes simplex virus UL33 gene product is required for the assembly of full capsids. Virology 180, 380-388.[CrossRef]
    [Google Scholar]
  4. Baer, R., Bankier, A. T., Biggin, M. D., Deininger, P. L., Farrell, P. J., Gibson, T. J., Hatfull, G., Hudson, G. S., Satchwell, S. C., Seguin, C., Tuffnell, P. S. & Barell, B. G. ( 1984; ). DNA sequence and expression of the B95-8 Epstein–Barr virus genome. Nature 310, 207-211.[CrossRef]
    [Google Scholar]
  5. Chee, M. S., Bankier, A. T., Beck, S., Bohni, R., Brown, C. M., Cerny, R., Horsnell, T., Hutchinson, C. A.III, Kouzarides, T., Martignetti, J. A., Preddie, E., Satchwell, S. C., Tomlinson, P., Weston, K. M. & Barrell, B. G. ( 1990; ). Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154, 125-169.
    [Google Scholar]
  6. Daikoku, T., Shibata, S., Goshima, F., Oshima, S., Tsurumi, T., Yamada, H., Yamashita, Y. & Nishiyama, Y. ( 1997; ). Purification and characterization of the protein kinase encoded by the UL13 gene of herpes simplex virus type 2. Virology 235, 82-93.[CrossRef]
    [Google Scholar]
  7. Davison, A. J. & Scott, J. E. ( 1986; ). The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67, 1759-1816.[CrossRef]
    [Google Scholar]
  8. Dolan, A., Jamieson, F. E., Cunningham, C., Barnett, B. C. & McGeoch, D. J. ( 1998; ). The genome sequence of herpes simplex virus type 2. Journal of Virology 72, 2010-2021.
    [Google Scholar]
  9. Gompels, U. A., Nicholas, J., Lawrence, G., Jones, M., Thomson, B. J., Martin, M. E. D., Efstathiou, S., Craxton, M. & Macaulay, H. A. ( 1995; ). The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution. Virology 209, 29-51.[CrossRef]
    [Google Scholar]
  10. Lamberti, C. & Weller, S. K. ( 1998; ). The herpes simplex virus type 1 cleavage/packaging protein, UL32, is involved in efficient localization of capsids to replication compartments. Journal of Virology 72, 2463-2473.
    [Google Scholar]
  11. McGeoch, D. J. ( 1992; ). Molecular evolution of large DNA viruses of eukaryotes. Seminars in Virology 3, 399-408.
    [Google Scholar]
  12. McGeoch, D. J., Dalrymple, M. A., Davison, A. J., Dolan, A., Frame, M. C., McNab, D., Perry, L. J., Scott, J. E. & Taylor, P. ( 1988; ). The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69, 1531-1574.[CrossRef]
    [Google Scholar]
  13. Patel, A. H. & MacLean, J. B. ( 1995; ). The product of the UL6 gene of herpes simplex virus type 1 is associated with virus capsids. Virology 206, 465-478.[CrossRef]
    [Google Scholar]
  14. Poon, A. P. W. & Roizman, B. ( 1993; ). Characterization of a temperature-sensitive mutant of the UL15 open reading frame of herpes simplex virus 1. Journal of Virology 67, 4497-4503.
    [Google Scholar]
  15. Rixon, F. J. ( 1993; ). Structure and assembly of herpesviruses. Seminars in Virology 4, 135-144.[CrossRef]
    [Google Scholar]
  16. Roizman, B. ( 1996; ). The function of herpes simplex virus genes: a primer for genetic engineering of novel vectors. Proceedings of the National Academy of Sciences, USA 93, 11307-11312.[CrossRef]
    [Google Scholar]
  17. Roizman, B. & Sears, A. E. ( 1996; ). Herpes simplex viruses and their replication. In Fields Virology, pp. 1043-1107. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  18. Salmon, B., Cunningham, C., Davison, A. J., Harris, W. J. & Baines, J. D. ( 1998; ). The herpes simplex virus type 1 UL17 gene encodes virion tegument proteins that are required for cleavage and packaging of viral DNA. Journal of Virology 72, 3779-3788.
    [Google Scholar]
  19. Schaffer, P. A., Aron, G. M., Biswal, N. & Benyesh-Melnick, M. ( 1973; ). Temperature-sensitive mutants of herpes simplex virus type 1: isolation, complementation and partial characterization. Virology 52, 57-71.[CrossRef]
    [Google Scholar]
  20. Sherman, G. & Bachenheimer, S. L. ( 1987; ). DNA processing in temperature-sensitive morphogenic mutants of HSV-1. Virology 158, 427-430.[CrossRef]
    [Google Scholar]
  21. Steven, A. C. & Spear, P. G. ( 1996; ). Herpesvirus capsid assembly and envelopment. In Structural Biology of Viruses, pp. 312-351. Edited by R. Burnet, W. Chiu & R. Garcea. New York: Oxford University Press.
  22. Tatman, J. D., Preston, V. G., Nicholson, P., Elliott, R. M. & Rixon, F. J. ( 1994; ). Assembly of herpes simplex virus type 1 capsids using a panel of recombinant baculoviruses. Journal of General Virology 75, 1101-1113.[CrossRef]
    [Google Scholar]
  23. Thomsen, D. R., Roof, L. L. & Homa, F. L. ( 1994; ). Assembly of herpes simplex virus (HSV) intermediate capsids in insect cells infected with recombinant baculoviruses expressing HSV capsid proteins. Journal of Virology 68, 2442-2457.
    [Google Scholar]
  24. Tsurumi, T., Maeno, K. & Nishiyama, Y. ( 1986; ). Molecular cloning of herpes simplex virus type 2 DNA. Journal of Biochemistry 99, 981-984.
    [Google Scholar]
  25. Ward, P. L., Ogle, W. O. & Roizman, B. ( 1996; ). Assemblons: nuclear structures defined by aggregation of immature capsids and some tegument proteins of herpes simplex virus 1. Journal of Virology 70, 4623-4631.
    [Google Scholar]
  26. Weller, S. K., Carmichael, E. P., Aschman, D. P., Goldstein, D. J. & Schaffer, P. A. ( 1987; ). Genetic and phenotypic characterization of mutants in four essential genes that map to the left half of HSV-1 UL DNA. Virology 161, 198-210.[CrossRef]
    [Google Scholar]
  27. Yu, D. & Weller, S. K. ( 1998; ). Herpes simplex virus type 1 cleavage and packaging proteins UL15 and UL28 are associated with B but not C capsids during packaging. Journal of Virology 72, 7428-7439.
    [Google Scholar]
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