1887

Abstract

The enterovirus echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the αβ integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this echovirus infection is controlled by the availability of αβ integrin.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-9-2311
1999-09-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/9/0802311a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-9-2311&mimeType=html&fmt=ahah

References

  1. Blaschuk K. L., Guerin C., Holland P. C. 1997; Myoblast αvβ3 integrin levels are controlled by transcriptional regulation of expression of the β3 subunit and down-regulation of β3 subunit expression is required for skeletal muscle cell differentiation. Developmental Biology 184:266–277
    [Google Scholar]
  2. Bültmann B. D., Eggers H. J., Galle J., Haferkamp O. 1983; Age dependence of paralysis induced by echovirus type 9 in infant mice. Journal of Infectious Diseases 147:999–1005
    [Google Scholar]
  3. Eggers H. J., Sabin A. B. 1959; Factors determining pathogenicity of variants of ECHO 9 virus for newborn mice. Journal of Experimental Medicine 110:951–967
    [Google Scholar]
  4. Goldberg R. J., Crowell R. L. 1971; Susceptibility of differentiating muscle cells of the fetal mouse in culture to coxsackievirus A13. Journal of Virology 7:759–769
    [Google Scholar]
  5. Melnick J. L. 1996; Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology pp 655–712 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  6. Menko A. S., Boettiger D. 1987; Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation. Cell 51:51–57
    [Google Scholar]
  7. Morens D. M., Pallansch M. A., Moore M. 1991; Polioviruses and other enteroviruses. In Textbook of Human Virology pp 427–497 Edited by Belshe R. B. St Louis: Mosby Year Book;
    [Google Scholar]
  8. Neff S., Sa-Carvalho D., Rieder E., Mason P. W., Blystone S. D., Brown E. J., Baxt B. 1998; Foot-and-mouth disease virus virulent for cattle utilizes the integrin αvβ3 as its receptor. Journal of Virology 72:3587–3594
    [Google Scholar]
  9. Nelsen-Salz B., Schildgen O., Klein M., Hadaschik D., Eggers H. J., Zimmermann H. 1999; Determinants of pathogenicity of echovirus 9 in men: significance of a functional RGD motif. Zentralblatt für Bakteriologie 289:347–354
    [Google Scholar]
  10. Pulli T., Koivunen E., Hyypiä T. 1997; Cell-surface interactions of echovirus 22. Journal of Biological Chemistry 272:21176–21180
    [Google Scholar]
  11. Ramos-Alvarez M., Sabin A. B. 1954; Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proceedings of the Society for Experimental Biology and Medicine 87:655–661
    [Google Scholar]
  12. Roivainen M., Piirainen L., Hovi T., Virtanen I., Riikonen T., Heino J., Hyypiä T. 1994; Entry of coxsackievirus A9 into host cells: specific interactions with αvβ3 integrin, the vitronectin receptor. Virology 203:357–365
    [Google Scholar]
  13. Rosenwirth B., Eggers H. J. 1982; Biochemistry and pathogenicity of echovirus 9. I. Characterization of the virus particles of strains Barty and Hill. Virology 123:102–112
    [Google Scholar]
  14. Zimmermann H., Eggers H. J., Zimmermann A., Kraus W., Nelsen-Salz B. 1995; Complete nucleotide sequence and biological properties of an infectious clone of prototype echovirus 9. Virus Research 39:311–319
    [Google Scholar]
  15. Zimmermann H., Eggers H. J., Nelsen-Salz B. 1996; Molecular cloning and sequence determination of the complete genome of the virulent echovirus 9 strain Barty. Virus Genes 12:149–154
    [Google Scholar]
  16. Zimmermann H., Eggers H. J., Nelsen-Salz B. 1997; Cell attachment and mouse virulence of echovirus 9 correlate with an RGD motif in the capsid protein VP1. Virology 233:149–156
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-80-9-2311
Loading
/content/journal/jgv/10.1099/0022-1317-80-9-2311
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error