1887

Abstract

Equine herpesvirus type 4 (EHV-4) is a major respiratory pathogen of horses. Unlike most other members of the , EHV-4 was regarded as non-neurotropic. Here, neural and lymphoid tissues of 17 horses have been analysed post-mortem. EHV-4 DNA was detected in 11 cases (65%) by PCR, exclusively in the trigeminal ganglia. In order to define the transcriptional activity, RNA preparations of 10 EHV-4 DNA-positive ganglia were investigated by nested RT–PCR. EHV-4-specific transcripts derived from genes 63 [herpes simplex virus type 1 (HSV-1) ICP0 gene homologue] and 64 (HSV-1 ICP4 gene homologue) were detected in six trigeminal ganglia. In one other case, only gene 64-specific transcripts were present. All of the transcripts proved to be antisense orientated when a strand-specific RT–PCR was applied. Type-specific primers for gene 33 (encoding glycoprotein B) served to detect transcripts of an acute EHV-4-infection, which were found in only one of the six ganglia positive for gene 63- and gene 64-specific transcripts. Overall, these studies clearly demonstrate that EHV-4 is latent in trigeminal ganglia.

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1999-08-01
2019-10-23
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References

  1. Allen, G. P. & Bryans, J. T. ( 1986; ). Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Progress in Veterinary Microbiology and Immunology 2, 78-144.
    [Google Scholar]
  2. Bartels, T., Steinbach, F., Hahn, G., Ludwig, H. & Borchers, K. ( 1998; ). In situ study on the pathogenesis and immune reaction of equine herpesvirus type 1 (EHV-1) infections in mice. Immunology 93, 329-334.
    [Google Scholar]
  3. Baxi, M. K., Efstathiou, S., Lawrence, G., Whalley, J. M., Slater, J. D. & Field, H. J. ( 1995; ). The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons. Journal of General Virology 76, 3113-3118.[CrossRef]
    [Google Scholar]
  4. Borchers, K., Wolfinger, U., Lawrenz, B., Schellenbach, A. & Ludwig, H. ( 1997; ). Equine herpesvirus 4 DNA in trigeminal ganglia of naturally infected horses detected by direct in situ PCR. Journal of General Virology 78, 1109-1114.
    [Google Scholar]
  5. Borchers, K., Wolfinger, U., Schellenbach, A., Lawrenz, B., Glitz, F. & Ludwig, H. ( 1999; ). Equid herpesvirus type 1 (EHV-1) and trigeminal ganglia of naturally infected horses: detection of DNA and latency associated transcripts (LATs) by RT-PCR. In Proceedings of the 18th International Conference on Equine Infectious Diseases, pp. 147-152. Edited by U. Wernery, J. F. Wade, J. A. Mumford & O.-R. Kaaden. Newmarket: R & W Publications.
  6. Bowles, D. E., Holden, V. R., Zhao, Y. & O’Callaghan, D. J. ( 1997; ). The ICP0 protein of equine herpesvirus 1 is an early protein that independently transactivates expression of all classes of viral promoters. Journal of Virology 71, 4904-4914.
    [Google Scholar]
  7. Cantello, J. L., Anderson, A. S. & Morgan, R. W. ( 1994; ). Identification of latency-associated transcripts that map antisense to the ICP4 homolog gene of Marek’s disease virus. Journal of Virology 68, 6280-6290.
    [Google Scholar]
  8. Chesters, P. M., Allsop, R., Purewal, A. & Edington, N. ( 1997; ). Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia. Journal of Virology 71, 3437-3443.
    [Google Scholar]
  9. Cheung, A. K. ( 1989; ). Detection of pseudorabies virus transcripts in trigeminal ganglia of latently infected swine. Journal of Virology 63, 2908-2913.
    [Google Scholar]
  10. Cheung, A. K. ( 1991; ). Cloning of the latency gene and the early protein 0 gene of pseudorabies virus. Journal of Virology 65, 5260-5271.
    [Google Scholar]
  11. Dobson, A. T., Sederati, F., Devi-Rao, G., Flanagan, W. M., Farrell, M. J., Stevens, J. G., Wagner, E. K. & Feldman, L. T. ( 1989; ). Identification of the latency-associated transcript promoter by expression of rabbit beta-globin mRNA in mouse sensory nerve ganglia latently infected with a recombinant herpes simplex virus. Journal of Virology 63, 3844-3851.
    [Google Scholar]
  12. Edington, N., Welch, H. M. & Griffiths, L. ( 1994; ). The prevalence of latent equid herpesviruses in the tissues of 40 abattoir horses. Equine Veterinary Journal 26, 140-142.[CrossRef]
    [Google Scholar]
  13. Gaskell, R. M., Dennis, P. E., Goddard, L. E., Cocker, F. M. & Wills, J. M. ( 1985; ). Isolation of felid herpesvirus I from the trigeminal ganglia of latently infected cats. Journal of General Virology 66, 391-394.[CrossRef]
    [Google Scholar]
  14. Jones, C. ( 1998; ). Alphaherpesvirus latency: its role in disease and survival of the virus in nature. Advances in Virus Research 51, 81-133.
    [Google Scholar]
  15. Kawaguchi, Y., Maeda, K., Miyazawa, T., Ono, M., Kai, C. & Mikami, T. ( 1994; ). Nucleotide sequence and characterization of the feline herpesvirus type 1 immediately early gene. Virology 204, 430-435.[CrossRef]
    [Google Scholar]
  16. Kritas, S. K., Pensaert, M. B. & Mettenleiter, T. C. ( 1994; ). Role of envelope glycoproteins gI, gp63 and gIII in the invasion and spread of Aujeszky’s disease virus in the olfactory nervous pathway of the pig. Journal of General Virology 75, 2319-2327.[CrossRef]
    [Google Scholar]
  17. Kritas, S. K., Nauwynck, H. J. & Pensaert, M. B. ( 1995; ). Dissemination of wild-type and gC-, gE- and gI-deleted mutants of Aujeszky’s disease virus in the maxillary nerve and trigeminal ganglion of pigs after intranasal inoculation. Journal of General Virology 76, 2063-2066.[CrossRef]
    [Google Scholar]
  18. Kutish, G., Mainprize, T. & Rock, D. ( 1990; ). Characterization of the latency-related transcriptionally active region of the bovine herpesvirus 1 genome. Journal of Virology 64, 5730-5737.
    [Google Scholar]
  19. Li, D.-S., Pastorek, J., Zelník, V., Smith, G. D. & Ross, L. J. N. ( 1994; ). Identification of novel transcripts complementary to the Marek’s disease virus homologue of the ICP4 gene of herpes simplex virus. Journal of General Virology 75, 1713-1722.[CrossRef]
    [Google Scholar]
  20. Matsumura, T., Sugiura, T., Imagawa, H., Fukunaga, Y. & Kamada, M. ( 1992; ). Epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations. Journal of Veterinary Medical Science 54, 207-211.[CrossRef]
    [Google Scholar]
  21. Mellerick, D. M. & Fraser, N. W. ( 1987; ). Physical state of the latent herpes simplex virus genome in a mouse model system: evidence suggesting an episomal state. Virology 158, 265-275.[CrossRef]
    [Google Scholar]
  22. Mitchell, W. J., Deshmane, S. L., Dolan, A., McGeoch, D. J. & Fraser, N. W. ( 1990; ). Characterization of herpes simplex virus type 2 transcription during latent infection of mouse trigeminal ganglia. Journal of Virology 64, 5342-5348.
    [Google Scholar]
  23. Ohlinger, V. F., Heck, R., Behrens, P., Rziha, H.-J., Weiland, F. & Wittmann, G. ( 1987; ). Die Infektion von Zellen des hämatopoetischen Systems durch das Aujeszkyvirus – Neue Aspekte für Immunbiologie und Praxis? Tierärztliche Umschau 3, 210-219.
    [Google Scholar]
  24. Ohmura, Y., Ono, E., Matsuura, T., Kida, H. & Shimizu, Y. ( 1993; ). Detection of feline herpesvirus 1 transcripts in trigeminal ganglia of latently infected cats. Archives of Virology 129, 341-347.[CrossRef]
    [Google Scholar]
  25. Rock, D. L., Hagemoser, W. A., Osorio, F. A. & Reed, D. E. ( 1986; ). Detection of bovine herpesvirus type 1 RNA in trigeminal ganglia of latently infected rabbits by in situ hybridization. Journal of General Virology 67, 2515-2520.[CrossRef]
    [Google Scholar]
  26. Rock, D. L., Nesburn, A. B., Ghiasi, H., Ong, J., Lewis, T. L., Lokensgard, J. R. & Wechsler, S. L. ( 1987; ). Detection of latency-related viral RNAs in trigeminal ganglia of rabbits latently infected with herpes simplex virus type 1. Journal of Virology 61, 3820-3826.
    [Google Scholar]
  27. Roizman, B. & Sears, A. E. ( 1987; ). An inquiry into the mechanisms of herpes simples virus latency. Annual Reviews in Microbiology 41, 543-571.[CrossRef]
    [Google Scholar]
  28. Shek, W. R., Calnek, B. W., Schat, K. A. & Chen, C. H. ( 1983; ). Characterization of Marek’s disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells. Journal of the National Cancer Institute 70, 485-491.
    [Google Scholar]
  29. Slater, J. D., Borchers, K., Thackray, A. M. & Field, H. J. ( 1994; ). The trigeminal ganglion is a location for equine herpesvirus 1 latency and reactivation in the horse. Journal of General Virology 75, 2007-2016.[CrossRef]
    [Google Scholar]
  30. Smith, D. J., Iqbal, J., Purewal, A., Hamblin, A. S. & Edington, N. ( 1998; ). In vitro reactivation of latent equid herpesvirus-1 from CD5+/CD8+ leukocytes indirectly by IL-2 or chorionic gonadotrophin. Journal of General Virology 79, 2997-3004.
    [Google Scholar]
  31. Stroop, W. G., Rock, D. L. & Fraser, N. W. ( 1984; ). Localization of herpes simplex virus in the trigeminal and olfactory systems of the mouse central nervous system during acute and latent infections by in situ hybridization. Laboratory Investigations 51, 27-38.
    [Google Scholar]
  32. Telford, E. A. R., Watson, M. S., McBride, K. & Davison, A. J. ( 1992; ). The DNA sequence of equine herpesvirus-1. Virology 189, 304-316.[CrossRef]
    [Google Scholar]
  33. Telford, E. A. R., Watson, M. S., Perry, J., Cullinane, A. A. & Davison, A. J. ( 1998; ). The DNA sequence of equine herpesvirus-4. Journal of General Virology 79, 1197-1203.
    [Google Scholar]
  34. Tenser, B. R., Edris, W. A., Hay, K. A. & de Galan, B. E. ( 1991; ). Expression of herpes simplex virus type 2 latency-associated transcript in neurons and nonneurons. Journal of Virology 65, 2745-2750.
    [Google Scholar]
  35. Wagner, E. K., Devi-Rao, G., Feldman, L. T., Dobson, A. T., Zhang, Y.-F., Flanagan, W. M. & Stevens, J. G. ( 1988; ). Physical characterization of the herpes simplex virus latency-associated transcript in neurons. Journal of Virology 62, 1194-1202.
    [Google Scholar]
  36. Welch, H. M., Bridges, C. G., Lyon, A. M., Griffiths, L. & Edington, N. ( 1992; ). Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues. Journal of General Virology 73, 261-268.[CrossRef]
    [Google Scholar]
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