%0 Journal Article %A Mundt, Egbert %T Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2 %D 1999 %J Journal of General Virology, %V 80 %N 8 %P 2067-2076 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-80-8-2067 %I Microbiology Society, %X Two types of strains of serotype I of infectious bursal disease virus (IBDV) have been described, on the basis of their ability (IBDV-TC) or inability (IBDV-BU) to infect chicken embryonic cells in culture. However, both types infect B lymphocytes in the bursa of Fabricius of young chickens. To determine the molecular basis for tissue culture infectivity, virus recombinants with chimeric segments A were constructed from IBDV-TC and IBDV-BU by reverse genetics. The region responsible for the different phenotypes was located in VP2. Site-directed mutagenesis identified single amino acids that are responsible for the restriction in infectivity. However, the appropriate amino acid exchanges are strain-specific. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-80-8-2067