RT Journal Article SR Electronic(1) A1 Björling, Ewa A1 von Garrelts, Eva A1 Mörner, Andreas A1 Ehnlund, Mariethe A1 Persson, Mats A. A. YR 1999 T1 Human neutralizing human immunodeficiency virustype 2-specific Fab molecules generated by phage display JF Journal of General Virology, VO 80 IS 8 SP 1987 OP 1993 DO https://doi.org/10.1099/0022-1317-80-8-1987 PB Microbiology Society, SN 1465-2099, AB A panel of human immunodeficiency virus type 2 (HIV-2)-neutralizing, recombinant Fab fragments was generated by using the phage display technique. The combinatorial library was derived from an asymptomatic, HIV-2-seropositive individual and constructed on the surface of filamentous phage by using the pComb3 phagemid vector and then screened against native HIV-2 envelope glycoprotein (gp125). Ten of 30 Fab fragments generated displayed strong reactivity in an ELISA and were therefore selected for further study. Six of these possessed neutralizing capacity, with titres varying from 20 to 80 against the homologous HIV-2 strain, and one also had a weak neutralizing capacity against a heterologous HIV-2 isolate, K135. Sequencing of the heavy chain CDR3 regions showed that the gp125-specific Fabs represented individual clones. These reagents may be useful for studies on the conformational structures of the HIV-2 envelope antigens and their immunogenicity, which may help in vaccine design. Furthermore, the cloned Fab genes may be transformed into whole IgG for eukaryotic expression, and as such used for therapeutic and immunoprophylactic studies in HIV-2-infected macaques and, possibly, for human immunoprophylaxis against HIV-2., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-80-8-1987