1887

Abstract

Human immunodeficiency virus (HIV) reverse transcription is an error-prone process with an overall mutation rate of ∼3·4×10 per base per replication cycle. This rate can be modulated by changes in different components of the retrotranscription reaction. In particular, substitution of magnesium cations (Mg) by manganese cations (Mn) has been shown to increase misincorporation of deoxynucleotide triphosphates (dNTPs) and to alter substrate specificity. Here, it is shown that Mn also increases the HIV mutation rate . Treatment of permissive cells with Mn and subsequent HIV infection resulted in at least 6-fold and 10-fold increases in the mutant and mutation frequencies respectively, thus illustrating a further example of how to influence HIV genetic variation.

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1999-08-01
2019-10-15
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