1887

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Volume 80, Issue 8

Mutations in the gene of human immunodeficiency virus type 1 NDK isolates and the use of African green monkey CXCR4 as a co-receptor in COS-7 cells Free

Abstract

A previous report from this laboratory described the isolation of the first CD4-independent human immunodeficiency virus type 1 isolate, m7NDK. This independence of CD4 is due to seven mutations located in the C2, V3 and C3 regions of the gp120 protein. The present report describes the entry features of the m5NDK virus, which contains five of the seven m7NDK mutations, located in the V3 loop and C3 region. The entry of this virus is strictly CD4-dependent but it can fuse with African green monkey (agm) COS-7 cells bearing human CD4 (h-CD4). This fusion is directly due to the five mutations in the gene. It has also been shown that entry of m7NDK is CD4-independent in COS-7 cells. Since the wild-type NDK and m7NDK viruses use the human CXCR4 protein as co-receptor, agm-CXCR4 was cloned and used in transfection and fusion inhibition experiments to show that this receptor can be used by the m5 and m7NDK viruses. The wild-type NDK virus, which does not enter COS-7 cells, can use agm-CXCR4, but only when the receptor is transfected into target cells. Although co-receptor nature and expression levels are still major determinants of virus entry, this is the first case where a few mutations in the gene can overcome this restriction.

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© Microbiology Society
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1999-08-01
2024-04-19
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References

  1. Adra C. N., Boer P. H., McBurney M. W. 1987; Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Gene 60:65–74
     [Google Scholar]
  2. Alkhatib G., Combadiere C., Broder C. C., Feng Y., Kennedy P. E., Murphy P. M., Berger E. A. 1996; CC CKR5: a RANTES, MIP-1α, MIP-1β receptor as a fusion cofactor for macrophage-tropic HIV-1. Science 272:1955–1958
     [Google Scholar]
  3. Alkhatib G., Liao F., Berger E. A., Farber J. M., Peden K. W. C. 1997; A new SIV co-receptor, STRL33. Nature 388:238
     [Google Scholar]
  4. Berger E. A., Doms R. W., Fenyo E. M., Korber B. T. M., Littman D. R., Moore J. P., Sattentau Q. J., Schuitemaker H., Sodroski J., Weiss R. A. 1998; A new classification for HIV-1. Nature 391:240
     [Google Scholar]
  5. Berson J. F., Doms R. W. 1998; Structure–function studies of the HIV-1 coreceptors. Seminars in Immunology 10:237–248
     [Google Scholar]
  6. Berson J. F., Long D., Doranz B. J., Rucker J., Jirik F. R., Doms R. W. 1996; A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus type 1 strains. Journal of Virology 70:6288–6295
     [Google Scholar]
  7. Bieniasz P. D., Fridell R. A., Anthony K., Cullen B. R. 1997; Murine CXCR-4 is a functional coreceptor for T-cell-tropic and dual-tropic strains of human immunodeficiency virus type 1. Journal of Virology 71:7097–7100
     [Google Scholar]
  8. Choe H., Farzan M., Konkel M., Martin K., Sun Y., Marcon L., Cayabyab M., Berman M., Dorf M. E., Gerard N., Gerard C., Sodroski J. 1998; The orphan seven-transmembrane receptor apj supports the entry of primary T-cell-line-tropic and dualtropic human immunodeficiency virus type 1. Journal of Virology 72:6113–6118
     [Google Scholar]
  9. Clapham P. R., McKnight A., Weiss R. A. 1992; Human immunodeficiency virus type 2 infection and fusion of CD4-negative human cell lines: induction and enhancement by soluble CD4. Journal of Virology 66:3531–3537
     [Google Scholar]
  10. Combadiere C., Salzwedel K., Smith E. D., Tiffany H. L., Berger E. A., Murphy P. M. 1998; Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1. Journal of Biological Chemistry 273:23799–23804
     [Google Scholar]
  11. Deng H., Liu R., Ellmeier W., Choe S., Unutmaz D., Burkhart M., Di Marzio P., Marmon S., Sutton R. E., Hill C. M., Davis C. B., Peiper S. C., Schall T. J., Littman D. R., Landau N. R. 1996; Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661–666
     [Google Scholar]
  12. Deng H. K., Unutmaz D., KewalRamani V. N., Littman D. R. 1997; Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 388:296–300
     [Google Scholar]
  13. Dimitrov D. S. 1997; How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity. Cell 91:721–730
     [Google Scholar]
  14. Doms R. W., Peiper S. C. 1997; Unwelcomed guests with master keys: how HIV uses chemokine receptors for cellular entry. Virology 235:179–190
     [Google Scholar]
  15. Dragic T., Alizon M. 1993; Different requirements for membrane fusion mediated by the envelopes of human immunodeficiency virus types 1 and 2. Journal of Virology 67:2355–2359
     [Google Scholar]
  16. Dragic T., Litwin V., Allaway G. P., Martin S. R., Huang Y. X., Nagashima K. A., Cayanan C., Maddon P. J., Koup R. A., Moore J. P., Paxton W. A. 1996; HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature 381:667–673
     [Google Scholar]
  17. Dumonceaux J., Nisole S., Chanel C., Quivet L., Amara A., Baleux F., Briand P., Hazan U. 1998; Spontaneous mutations in the env gene of the human immunodeficiency virus type 1 NDK isolate are associated with a CD4-independent entry phenotype. Journal of Virology 72:512–519
     [Google Scholar]
  18. Endres M. J., Clapham P. R., Marsh M., Ahuja M., Turner J. D., McKnight A., Thomas J. F., Stoebenau-Haggarty B., Choe S., Vance P. J., Wells T. N. C., Power C. A., Sutterwala S. S., Doms R. W., Landau N. R., Hoxie J. A. 1996; CD4-independent infection by HIV-2 is mediated by fusin/CXCR-4. Cell 87:745–756
     [Google Scholar]
  19. Farzan M., Choe H., Martin K., Marcon L., Hofmann W., Karlsson G., Sun Y., Barrett P., Marchand N., Sullivan N., Gerard N., Gerard C., Sodroski J. 1997; Two orphan seven-transmembrane segment receptors which are expressed in CD4-positive cells support simian immunodeficiency virus infection. Journal of Experimental Medicine 186:405–411
     [Google Scholar]
  20. Feng Y., Broder C. C., Kennedy P. E., Berger E. A. 1996; HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 272:872–877
     [Google Scholar]
  21. Gossen M., Bujard H. 1992; Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proceedings of the National Academy of Sciences USA 89:5547–5551
     [Google Scholar]
  22. He J., Chen Y., Farzan M., Choe H., Ohagen A., Gartner S., Busciglio J., Yang X., Hofmann W., Newman W., Mackay C. R., Sodroski J., Gabuzda D. 1997; CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature 385:645–649
     [Google Scholar]
  23. Himathongkham S., Luciw P. A. 1996; Restriction of HIV-1 (subtype B) replication at the entry step in rhesus macaque cells. Virology 219:485–488
     [Google Scholar]
  24. Kozak S. L., Platt E. J., Madani N., Ferro F. E. Jr, Peden K., Kabat D. 1997; CD4, CXCR-4, and CCR-5 dependencies for infections by primary patient and laboratory-adapted isolates of human immunodeficiency virus type 1. Journal of Virology 71:873–882
     [Google Scholar]
  25. Li X. L., Moudgil T., Vinters H. V., Ho D. D. 1990; CD4-independent, productive infection of a neuronal cell line by human immunodeficiency virus type 1. Journal of Virology 64:1383–1387
     [Google Scholar]
  26. Liao F., Alkhatib G., Peden K. W. C., Sharma G., Berger E. A., Farber J. M. 1997; STRL33, a novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. Journal of Experimental Medicine 185:2015–2023
     [Google Scholar]
  27. Loetscher M., Geiser T., O’Reilly T., Zwahlen R., Baggiolini M., Moser B. 1994; Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. Journal of Biological Chemistry 269:232–237
     [Google Scholar]
  28. Moore J. P. 1997; Coreceptors: implications for HIV pathogenesis and therapy. Science 276:51–52
     [Google Scholar]
  29. Moore J. P., Trkola A., Dragic T. 1997; Co-receptors for HIV-1 entry. Current Opinion in Immunology 9:551–562
     [Google Scholar]
  30. Platt E. J., Wehrly K., Kuhmann S. E., Chesebro B., Kabat D. 1998; Effects of CCR5 and CD4 cell surface concentrations on infections by macrophagetropic isolates of human immunodeficiency virus type 1. Journal of Virology 72:2855–2864
     [Google Scholar]
  31. Pleskoff O., Sol N., Labrosse B., Alizon M. 1997a; Human immunodeficiency virus strains differ in their ability to infect CD4+ cells expressing the rat homolog of CXCR-4 (fusin. Journal of Virology 71:3259–3262
     [Google Scholar]
  32. Pleskoff O., Treboute C., Brelot A., Heveker N., Seman M., Alizon M. 1997b; Identification of a chemokine receptor encoded by human cytomegalovirus as a cofactor for HIV-1 entry. Science 276:1874–1878
     [Google Scholar]
  33. Reeves J. D., Schulz T. F. 1997; The CD4-independent tropism of human immunodeficiency virus type 2 involves several regions of the envelope protein and correlates with a reduced activation threshold for envelope-mediated fusion. Journal of Virology 71:1453–1465
     [Google Scholar]
  34. Reeves J. D., McKnight A., Potempa S., Simmons G., Gray P. W., Power C. A., Wells T., Weiss R. A., Talbot S. J. 1997; CD4-independent infection by HIV-2 (ROD/B): use of the 7-transmembrane receptors CXCR-4, CCR-3, and V28 for entry. Virology 231:130–134
     [Google Scholar]
  35. Rucker J., Edinger A. L., Sharron M., Samson M., Lee B., Berson J. F., Yi Y., Margulies B., Collman R. G., Doranz B. J., Parmentier M., Doms R. W. 1997; Utilization of chemokine receptors, orphan receptors, and herpesvirus-encoded receptors by diverse human and simian immunodeficiency viruses. Journal of Virology 71:8999–9007
     [Google Scholar]
  36. Sol N., Morinet F., Alizon M., Hazan U. 1993; Trans-activation of the long terminal repeat of human immunodeficiency virus type 1 by the parvovirus B19 NS1 gene product. Journal of General Virology 74:2011–2014
     [Google Scholar]
  37. Tachibana K., Nakajima T., Sato A., Igarashi K., Shida H., Iizasa H., Yoshida N., Yoshie O., Kishimoto T., Nagasawa T. 1997; CXCR4/fusin is not a species-specific barrier in murine cells for HIV-1 entry. Journal of Experimental Medicine 185:1865–1870
     [Google Scholar]
  38. Willett B. J., Picard L., Hosie M. J., Turner J. D., Adema K., Clapham P. R. 1997; Shared usage of the chemokine receptor CXCR4 by the feline and human immunodeficiency viruses. Journal of Virology 71:6407–6415
     [Google Scholar]
  39. Zhang L., Carruthers C. D., He T., Huang Y., Cao Y., Wang G., Hahn B., Ho D. D. 1997; HIV type 1 subtypes, coreceptor usage, and CCR5 polymorphism. AIDS Research and Human Retroviruses 13:1357–1366
     [Google Scholar]
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Mutations in the env gene of human immunodeficiency virus type 1 NDK isolates and the use of African green monkey CXCR4 as a co-receptor in COS-7 cells
J. Gen. Virol. 80, 1975 (1999); https://doi.org/10.1099/0022-1317-80-8-1975
/content/journal/jgv/10.1099/0022-1317-80-8-1975
/content/journal/jgv/10.1099/0022-1317-80-8-1975
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