1887

Abstract

In the course of experiments designed to assess the potential role of alternative open reading frames (ORF) present in the 5′-terminal untranslated region (5′-UTR) of poliovirus type 1 (Mahoney strain) genomic RNA, we came across a double mutation that completely abrogated the infectivity of full-length cDNA clones. The infectivity was rescued in by cotransfecting COS-1 cells with short RNA transcripts of the wild-type 5′-UTR of poliovirus type 2 Lansing, provided a free 3′-OH was available. Direct sequencing of the viral RNA revealed that the infectious viruses recovered were recombinants Lansing/Mahoney, with variable points of ‘crossing-over’. A novel mechanism of RNA–RNA recombination, which we propose to call ‘primer alignment-and-extension’, is described that would explain the high rate of recombination of RNA viruses observed in natural conditions.

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1999-08-01
2024-12-14
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