1887

Abstract

In the course of experiments designed to assess the potential role of alternative open reading frames (ORF) present in the 5′-terminal untranslated region (5′-UTR) of poliovirus type 1 (Mahoney strain) genomic RNA, we came across a double mutation that completely abrogated the infectivity of full-length cDNA clones. The infectivity was rescued in by cotransfecting COS-1 cells with short RNA transcripts of the wild-type 5′-UTR of poliovirus type 2 Lansing, provided a free 3′-OH was available. Direct sequencing of the viral RNA revealed that the infectious viruses recovered were recombinants Lansing/Mahoney, with variable points of ‘crossing-over’. A novel mechanism of RNA–RNA recombination, which we propose to call ‘primer alignment-and-extension’, is described that would explain the high rate of recombination of RNA viruses observed in natural conditions.

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1999-08-01
2019-10-19
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References

  1. Agol, V. I., Grachev, V. P., Drozdov, S. G., Kolesnikova, M. S., Koslov, V. G., Ralf, N. M., Romanova, L. I., Tolskaya, E. A., Tyufanov, A. V. & Viktorova, E. G. ( 1984; ). Construction and properties of intertypic poliovirus recombinants: first approximation mapping of the major determinants of neurovirulence. Virology 136, 41-55.[CrossRef]
    [Google Scholar]
  2. Bujarski, J. ( 1996; ). Experimental systems of genetic recombination and defective RNA formation in RNA viruses. Seminars in Virology 7, 361-362.[CrossRef]
    [Google Scholar]
  3. Bujarski, J. & Nagy, P. D. ( 1996; ). Different mechanisms of homologous and non-homologous recombination in brome mosaic virus: role of RNA sequences and replicase proteins. Seminars in Virology 7, 363-372.[CrossRef]
    [Google Scholar]
  4. Chetverin, A. B., Chetverina, H. V., Demidenko, A. A. & Ugarov, V. I. ( 1997; ). Non homologous RNA recombination in a cell-free system: evidence for a transesterification mechanism guided by secondary structure. Cell 88, 503-513.[CrossRef]
    [Google Scholar]
  5. Degener, A. M., Pagnotti, P., Facchini, J. & Perez Bercoff, R. ( 1983; ). Genomic RNA of mengovirus. VI. Translation of its two cistrons in lysates of interferon-treated cells. Journal of Virology 45, 889-894.
    [Google Scholar]
  6. Degener, A. M., Silveira Carneiro, J., Cassetti, C., Pierangeli, A., Pagnotti, P., Bucci, M. & Perez Bercoff, R. ( 1995; ). Role of the pyrimidine-rich tract on the translation of chimeric polio–hepatitis A mRNAs with engineered 5′-terminal untranslated region. Virus Research 37, 291-303.[CrossRef]
    [Google Scholar]
  7. Devereux, J., Haeberli, P. & Smithies, O. ( 1985; ). A comprehensive set of sequence analysis of programs for the VAX. Nucleic Acids Research 12, 387-395.
    [Google Scholar]
  8. Hirst, G. K. ( 1962; ). Genetic recombination with Newcastle disease virus, poliovirus, and influenza. Cold Spring Harbor Symposia on Quantitative Biology 27, 303-308.[CrossRef]
    [Google Scholar]
  9. Jang, S. K. & Wimmer, E. ( 1990; ). Cap-independent translation of encephalomyocarditis virus RNA: structural elements of the internal ribosomal entry site and involvement of a cellular 57 KDa RNA-binding protein. Genes & Development 4, 1560-1572.[CrossRef]
    [Google Scholar]
  10. Kirkegaard, K. & Baltimore, D. ( 1986; ). The mechanism of RNA recombination in poliovirus. Cell 47, 433-443.[CrossRef]
    [Google Scholar]
  11. Lai, M. M. C. ( 1992; ). RNA recombination in animal and plant viruses. Microbiological Reviews 56, 61-79.
    [Google Scholar]
  12. Lai, M. M. C. ( 1996; ). Recombination in large RNA viruses: coronaviruses. Seminars in Virology 7, 381-388.[CrossRef]
    [Google Scholar]
  13. Lipskaya, G. Y., Muzychenko, A. R., Kutikova, O. K., Maslova, S. V., Equestre, M., Drozdov, S. G., Perez Bercoff, R. & Agol, V. ( 1991; ). Frequent isolation of intertypic poliovirus recombinants with serotype 2 specificity from vaccine-associated polio cases. Journal of Medical Virology 35, 290-296.[CrossRef]
    [Google Scholar]
  14. Meerovitch, K., Pelletier, J. & Sonenberg, N. ( 1989; ). A cellular protein that binds to the 5′-non coding region of poliovirus RNA: implication for internal translation initiation. Genes & Development 3, 1026-1034.[CrossRef]
    [Google Scholar]
  15. Nicholson, R., Pelletier, J. & Sonenberg, N. ( 1991; ). Structural and functional analysis of the ribosome landing pad of poliovirus type 2: in vitro translation studies. Journal of Virology 65, 6886-6894.
    [Google Scholar]
  16. Pelletier, J. & Sonenberg, N. ( 1988; ). Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature 334, 426-429.
    [Google Scholar]
  17. Pelletier, J., Flynn, M. E., Kaplan, G., Racaniello, V. & Sonenberg, N. ( 1988; ). Mutational analysis of upstream AUG codons of poliovirus RNA. Journal of Virology 62, 4486-4492.
    [Google Scholar]
  18. Perez Bercoff, R. ( 1982; ). But is the 5′-end of messenger RNA always involved in initiation? In Protein Biosynthesis in Eukaryotes, pp. 245-252. Edited by R. Perez Bercoff. New York: Plenum.
  19. Perez Bercoff, R. ( 1987; ). Picornaviruses at the molecular level. In The Molecular Basis of Viral Replication, pp. 197-215. Edited by R. Perez Bercoff. New York: Plenum.
  20. Perez Bercoff, R. & Kaempfer, R. ( 1982; ). Genomic RNA of mengovirus. V. Recognition of common features by ribosomes and eukaryotic initiation factor eIF-2. Journal of Virology 41, 30-41.
    [Google Scholar]
  21. Pierangeli, A., Bucci, M., Pagnotti, P., Degener, A. & Perez Bercoff, R. ( 1995; ). Mutational analysis of the 3′-terminal extra-cistronic region of poliovirus RNA: secondary structure is not the only requirement for minus strand RNA replication. FEBS Letters 374, 327-332.
    [Google Scholar]
  22. Pierangeli, A., Bucci, M., Forzan, M., Pagnotti, P., Equestre, M. & Perez Bercoff, R. ( 1998; ). Identification of an alternative open reading frame (‘‘hidden gene’’?) stringently required for the infectivity of poliovirus cDNA clones. Microbiologica 21, 309-320.
    [Google Scholar]
  23. Rueckert, R. R. ( 1990; ). Picornaviridae: the viruses and their replication. In Virology, pp. 705-738. Edited by B. N. Fields, D. M. Knipe, R. M. Chanock, J. L. Melnick, B. Roizman & R. E. Shope. New York: Raven Press.
  24. Sambrook, J., Fritsch, F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  25. Silveira Carneiro, J., Equestre, M., Pagnotti, P., Gradi, A., Sonenberg, N. & Perez Bercoff, R. ( 1995; ). 5′-UTR of hepatitis A virus RNA: mutations in the 5′-most pyrimidine-rich tract reduce its ability to direct internal initiation of translation. Journal of General Virology 76, 1189-1196.[CrossRef]
    [Google Scholar]
  26. Tolskaya, E. A., Romanova, L. I., Kolesnikova, M. S. & Agol, V. I. ( 1983; ). Intertypic recombination in poliovirus: genetic and biochemical studies. Virology 124, 121-132.[CrossRef]
    [Google Scholar]
  27. van Marle, G., Luytjes, W., Snijder, E. J. & Spaan, W. J. M. (1998). Nidovirus discontinuous mRNA transcription involves base-pairing between the leader and promoter sequences. Abstracts of the Fifth International Symposium on Positive Strand RNA Viruses, St Petersburg, FL, USA, May 23–28.
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