1887

Abstract

The major mRNA species in the E1 region of the genome of bovine adenovirus type 3 (BAV3) have been defined by using a combination of PCR, 5′ RACE, Northern analysis and DNA sequencing. Independent transcription initiation sites were identified for each of the E1a, E1b and protein IX (pIX) transcription units, but all mRNA species terminated at the same poly(A) addition site immediately downstream of the pIX open reading frame. Thus, the BAV3 E1 region, which consists of the E1a and E1b genes together with that for pIX, functions as a nested overlapping transcription unit. One major mRNA species encoding the E1a protein was found and two mRNAs encoding E1b species, the smaller of which encodes the E1b 17K protein alone and the larger encodes both 17K and 47K E1b proteins, were identified. One mRNA species encodes pIX. The E1a transcript, encoding the predicted 214 residue E1a protein, has four exons. The smaller E1b mRNA has two exons, the second of which corresponds to the last exon of E1a. No introns were detected in the larger E1b mRNA that encodes both the E1b 17K and 47K proteins nor in the mRNA encoding pIX. The relative times of appearance of the mRNAs from the E1-pIX gene region following infection of bovine cells with BAV3 was determined.

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1999-07-01
2024-04-18
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References

  1. Ball A. O., Beard C. W., Redick S. D., Spindler K. R. 1989; Genome organization of mouse adenovirus type 1 early region 1: a novel transcription map. Virology 170:523–536
    [Google Scholar]
  2. Berk A. J., Sharp P. A. 1977; Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732
    [Google Scholar]
  3. Bos J. L., Polder L. J., Bernards R., Schrier P. I., van den Elsen P. J., van der Eb A. J., van Ormondt H. 1981; The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets. Cell 27:121–131
    [Google Scholar]
  4. Darbyshire J. H., Dawson P. S., Lamont R. H., Ostler D. C., Pereira G. H. 1965; A new adenovirus serotype of bovine origin. Journal of Comparative Pathology 75:327–330
    [Google Scholar]
  5. Elgadi M., Rghei N., Haj-Ahmad Y. 1993; Sequence and sequence analysis of E1 and pIX regions of the BAV3 genome. Intervirology 36:113–120
    [Google Scholar]
  6. Falck-Pedersen E., Logan J., Shenk T., Darnell J. E. Jr 1985; Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element. Cell 40:897–905
    [Google Scholar]
  7. Hall S. L., Padgett R. A. 1994; Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice sites. Journal of Molecular Biology 239:357–365
    [Google Scholar]
  8. Ishida S., Fujinaga Y., Fujinaga K., Sakamoto N., Hashimoto S. 1994; Unusual splice sites in the E1A-E1B cotranscripts synthesized in adenovirus type 40-infected A549 cells. Archives of Virology 139:389–402
    [Google Scholar]
  9. Kitchingman G. R., Westphal H. 1980; The structure of adenovirus 2 early nuclear and cytoplasmic RNAs. Journal of Molecular Biology 137:23–48
    [Google Scholar]
  10. Kozak M. 1987; Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Molecular and Cellular Biology 7:3438–3445
    [Google Scholar]
  11. Lehrach M., Diamond D., Wozney J. M., Boedtker H. 1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
    [Google Scholar]
  12. Mount S. M. 1996; Perspective: AT-AC introns: an ATtACk on dogma. Science 271:1690–1692
    [Google Scholar]
  13. Parks C. L., Banerjee S., Spector D. J. 1988; Organization of the transcriptional control region of the E1b gene of adenovirus type 5. Journal of Virology 62:54–67
    [Google Scholar]
  14. Saito I., Shiroki K., Shimojo H. 1983; mRNA species and proteins of adenovirus type 12 transforming regions: identification of proteins translated from multiple coding stretches in 2.2 kb region 1B mRNA in vitro and in vivo. Virology 127:272–289
    [Google Scholar]
  15. Shenk T. 1996; Adenoviridae: the viruses and their replication. In Fields Virology 3rd edn pp 2111–2148 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott-Raven;
    [Google Scholar]
  16. Spector D. J., Parks C. L., Knittle R. A. 1993; A multicomponent cis-activator of transcription of the E1b gene of adenovirus type 5. Virology 194:128–136
    [Google Scholar]
  17. Steinthorsdottir V., Mautner V. 1991; Enteric adenovirus type 40: E1B transcription map and identification of novel E1A-E1B cotranscripts in lytically infected cells. Virology 181:139–149
    [Google Scholar]
  18. Vales L. D., Darnell J. E. Jr 1989; Promoter occlusion prevents transcription of adenovirus polypeptide IX mRNA until after DNA replication. Genes & Development 3:49–59
    [Google Scholar]
  19. Wu L., Berk A. 1988; Constraints on spacing between transcription factor binding sites in a simple adenovirus promoter. Genes & Development 2:403–411
    [Google Scholar]
  20. Wu L., Rosser D. S., Schmidt M. C., Berk A. 1987; A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter. Nature 326:512–515
    [Google Scholar]
  21. Zheng B., Mittal S. K., Graham F. L., Prevec L. 1994; The E1sequence of bovine adenovirus type 3 and complementation of human adenovirus type 5 E1A function in bovine cells. Virus Research 31:163–186
    [Google Scholar]
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