RT Journal Article SR Electronic(1) A1 Bankamp, Bettina A1 Bellini, William J. A1 Rota, Paul A.YR 1999 T1 Comparison of L proteins of vaccine and wild-type measles viruses JF Journal of General Virology, VO 80 IS 7 SP 1617 OP 1625 DO https://doi.org/10.1099/0022-1317-80-7-1617 PB Microbiology Society, SN 1465-2099, AB The nucleotide sequences of the large (L) genes of ten measles virus (MV) strains were determined. These strains included the Moraten and Rubeovax vaccine strains and their Edmonston wild-type (wt) progenitor, two additional vaccine strains and five genotypically divergent wt isolates. The nucleotide and predicted amino acid sequences were compared with six previously sequenced L genes and the number and location of variable amino acid positions were characterized. The recent wt isolates demonstrated the greatest amount of variability found to date in the highly conserved L protein. Three full-length wt L proteins were expressed in mammalian cells and their ability to form a complex with the MV phosphoprotein was demonstrated. While no set of amino acid substitutions associated consistently with wt or vaccine strains was identified, these data will provide a basis for the analysis of the activity of L proteins from vaccine and wt viruses in a functional assay., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-80-7-1617