1887

Abstract

T cell epitopes of the measles virus (MV) nucleoprotein were studied by synthesizing overlapping 20 aa peptides over the known sequence of the protein and analysing the proliferation responses of a panel of MV-specific T cell lines and clones against these peptides. T cell lines were established from eleven healthy controls and seven multiple sclerosis patients, all with a history of past MV infection. The epitopes recognized by these lines were concentrated in a few regions of the polypeptide chain. Overlapping peptides containing aa 321-340 and 331-350 were most often recognized. Other epitopes were detected close to the amino-terminal end of the polypeptide chain as each of the peptides 1-20, 21-40, 31-50 and 51-70 contained stimulating moieties. Some responses were also detected towards peptides 151-200 and 221-250, but the carboxy-terminal end of the polypeptide was not recognized by any of the tested T cell lines. The amino acid sequences of the peptides that stimulated the T cell clones and lines, as a rule, contained binding motifs described for HLA-DR alleles found in T cell donors. The regions of protein sequence which did not reveal any T cell epitopes were, instead, relatively free of binding motifs. The results suggest that only a few epitopes of the MV nucleoprotein are important in establishing T cell immunity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-7-1609
1999-07-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/7/0801609a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-7-1609&mimeType=html&fmt=ahah

References

  1. Banos D. M., Lopez S., Arias C. F., Esquivel F. R. 1997; Identification of T-helper cell epitopes on the rotavirus VP6 protein. Journal of Virology 71:419–426
    [Google Scholar]
  2. Berzofsky J. A. 1988; Immunodominance in T lymphocyte recognition. Immunology Letters 18:83–92
    [Google Scholar]
  3. Burkhart C., Freer G., Castro R., Adorini L., Wiesmuller K.-H., Zinkernagel R. M., Hengartner H. 1994; Characterization of T-helper epitopes of the glycoprotein of vesicular stomatitis virus. Journal of Virology 68:1573–1580
    [Google Scholar]
  4. Callebaut I., Voneche V., Mager A., Fumiere O., Krchnak V., Merza M., Vada J. Z., Mammrickx M., Burny A., Portetelle D. 1993; Mapping of B-neutralizing and T-helper cell epitopes on the bovine leukemia virus external glycoprotein GP51. Journal of Virology 67:5321–5327
    [Google Scholar]
  5. Chicz R. M., Urban R. G., Gorga J. C., Vignali D. A. A., Lane W. S., Strominger J. L. 1993; Specificity and promiscuity among naturally processed peptides bound to HLA-DR alleles. Journal of Experimental Medicine 178:27–47
    [Google Scholar]
  6. Deng H., Fosdick L., Sercarz E. 1993; The involvement of antigen processing in determinant selection by class II MHC and its relationship to immunodominance. Acta Pathologica Microbiologica et Immunologica Scandinavica 101:655–662
    [Google Scholar]
  7. Fulginiti V. A., Eller J. J., Downie A. W., Kempe C. H. 1967; Altered reactivity to measles virus. Journal of the American Medical Society 202:1075–1080
    [Google Scholar]
  8. Garenne M., Aaby P. 1990; Pattern of exposure and measles mortality in Senegal. Journal of Infectious Diseases 161:1088–1094
    [Google Scholar]
  9. Gelder C., Davenport M., Barnardo M., Bourne T., Lamb J., Askonas B., Hill A., Welsh K. 1998; Six unrelated HLA-DR-matched adults recognize identical CD4+ T cell epitopes from influenza A haemagglutinin that are not simply peptides with high HLA-DR binding affinities. International Immunology 10:211–222
    [Google Scholar]
  10. Griffin D., Bellini W. 1996; Measles virus. In Fields Virology 3rd edn, pp 1267–1298 Edited by Fields B. N. Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  11. Hammer J., Valsasnini P., Tolba K., Bolin D., Higelin J., Takacs B., Sinigaglia F. 1993; Promiscuous and allele-specific anchors in HLA-DR-binding peptides. Cell 74:197–203
    [Google Scholar]
  12. Hickman C. J., Khan A. S., Rota P. A., Bellini W. J. 1997; Use of synthetic peptides to identify measles nucleoprotein T-cell epitopes in vaccinated and naturally infected humans. Virology 235:386–397
    [Google Scholar]
  13. Houghten R. A. 1985; General method for the rabid solid phase synthesis of large numbers of peptides: specificity of antigen-antibody interactions at the level of individual amino acids. Proceedings of the National Academy of Sciences, USA 82:5131–5153
    [Google Scholar]
  14. Houghten R. A., Bray M. K., DeGraw S. T., Kirby C. J. 1986; Simplified procedure for carrying out simultaneous multiple hydrogen fluoride cleavages of protected peptide resins. International Journal of Peptide and Protein Research 27:675–680
    [Google Scholar]
  15. Ilonen J. 1979; Lymphocyte blast transformation response of seropositive and seronegative subjects to herpes simplex, rubella, mumps and measles virus antigens. Acta Pathologica et Microbiologica Scandinavica Sect C Immunol 87:151–157
    [Google Scholar]
  16. Ilonen J., Mäkelä M. J., Ziola B., Salmi A. A. 1990; Cloning of human T cell specific for measles virus haemagglutinin and nucleocapsid. Clinical and Experimental Immunology 81:212–217
    [Google Scholar]
  17. Kantz S. L., Gellin B. G. 1994; Measles vaccine: do we need new vaccines or new programs?. Science 265:1391–1392
    [Google Scholar]
  18. Marttila J., Ilonen J., Lehtinen M., Parkkonen P., Salmi A. 1996; Definition of three minimal T helper cell epitopes of rubella virus E1 glycoprotein. Clinical and Experimental Immunology 104:394–397
    [Google Scholar]
  19. Nanan R., Carstens C., Kreth H. W. 1995; Demonstration of virus-specific CD8+ memory T cell in measles-seropositive individuals by in vitro peptide stimulation. Clinical and Experimental Immunology 102:40–45
    [Google Scholar]
  20. Nepom G. T., Domeier M. E., Ou D., Kovats S., Mitchell L. A., Tingle A. J. 1997; Recognition of contiguous allele-specific peptide elements in Rubella virus E1 envelope protein. Vaccine 15:648–652
    [Google Scholar]
  21. Norrby E., Oxman M. N. 1990; Measles virus. In Fields Virology 2nd edn, pp 1013–1037 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  22. Obeid E., Partidos C. D., Steward M. W. 1993; Identification of helper T cell antigenic sites in mice from the haemagglutinin glycoprotein of measles virus. Journal of General Virology 74:2549–2557
    [Google Scholar]
  23. Rammensee H.-G., Friede T., Stevanovic S. 1995; MHC ligands and peptide motifs: first listing. Immunogenetics 41:178–228
    [Google Scholar]
  24. Rota P. A., Bloom A. E., Vanchiere J. A., Bellini W. J. 1994; Evolution of the nucleoprotein and matrix genes of wild-type strains of measles virus isolated from recent epidemics. Virology 198:724–730
    [Google Scholar]
  25. Rozenblatt S., Eisenberg O., Ben-Levy R., Bellini W. J. 1985; Sequence homology within the morbilliviruses. Journal of Virology 53:684–690
    [Google Scholar]
  26. Tam J. P., Heath W. F., Merrifield R. B. 1983; Sn2 deprotection of synthetic peptides with a low concentration of HF in dimethyl sulfide: evidence and application in peptide synthesis. Journal of the American Chemical Society 105:6442–6451
    [Google Scholar]
  27. Taylor M. J., Godfrey E., Baczko K., ter Meulen V., Wild T. F., Rima B. K. 1991; Identification of several different lineages of measles virus. Journal of General Virology 72:83–88
    [Google Scholar]
  28. Vordermeier H.-M., Harris D. P., Friscia G., Roman E., Surcel H.-M., Moreno C., Pasvol G., Ivanyi J. 1992; T cell repertoire in tuberculosis: selective anergy to immunodominant epitope of the 38-kDa antigen in patients with active disease. European Journal ofImmunology 22:2631–2637
    [Google Scholar]
  29. Weiss R. 1992; Measles battle loses potent weapon. Science 258:546–547
    [Google Scholar]
  30. Wucherpfennig K. W., Strominger J. L. 1995; Molecular mimicry in T cell-mediated autoimmunity: viral peptides activate human T cell clones specific for myelin basic protein. Cell 80:695–705
    [Google Scholar]
  31. Yewdell J. W., Bennink J. R. 1990; The binary logic of antigen processing and presentation to T cells. Cell 62:203–206
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-7-1609
Loading
/content/journal/jgv/10.1099/0022-1317-80-7-1609
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error