Human papillomavirus (HPV) E6 interactions with Bak are conserved amongst E6 proteins from high and low risk HPV types Free

Abstract

Human papillomavirus (HPV) replication occurs in terminally differentiating epithelium, and requires the activation of cellular DNA replication proteins. Unscheduled DNA replication can result in the induction of apoptosis, and the viral E6 protein induces the degradation of p53 to prevent this. It has recently been shown that HPV-18 E6 can also stimulate the degradation of Bak, a pro-apoptotic member of the Bcl-2 family. This report shows that the E6 proteins from HPV-18, HPV-16 and HPV-11 can all bind to Bak , stimulate its degradation and reduce Bak-induced apoptosis. However, the non-oncogenic HPV-11 E6 is less effective than the oncogenic E6 proteins in each of these assays, indicating that the ability of HPV to circumvent the apoptosis induced by Bak may contribute to the oncogenic potential of the virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-6-1513
1999-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/6/0801513a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-6-1513&mimeType=html&fmt=ahah

References

  1. Bandara L., Adamczewski J., Hunt T., LaThangue N. 1991; Cyclin A and the retinoblastoma gene product complex with a common transcription factor. Nature 352:249–251
    [Google Scholar]
  2. Blanton R., Coltrera M., Gown A., Halbert C., McDougall J. 1992; Expression of the HPV16 E7 gene generates proliferation in stratified squamous cell cultures which is independent of endogenous p53 levels. Cell Growth & Differentiation 3:791–802
    [Google Scholar]
  3. Chellappan S., Kraus V., Kroger B., Munger K., Howley P., Phelps W., Nevins J. 1992; Adenovirus E1A, simian virus 40 tumour antigen and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. Proceedings of the National Academy of Sciences, USA 89:4549–4553
    [Google Scholar]
  4. Cheng S., Schmidt-Grimminger D., Murant T., Broker T., Chow L. 1995; Differentiation-dependent up-regulation of the human papillomavirus E7 gene reactivates cellular DNA replication in suprabasal differentiated keratinocytes. Genes & Development 9:2335–2349
    [Google Scholar]
  5. Chittenden T., Flemington C., Houghton A., Ebb R., Gallo G., Elangovan B., Chinnadurai G., Lutz R. 1995; A conserved domain in Bak, distinct from BH1 and BH2, mediates cell death and protein binding functions. EMBO Journal 14:5589–5596
    [Google Scholar]
  6. Doorbar J., Foo C., Coleman N., Medcalf L., Hartley O., Prospero T., Napthine S., Sterling J., Winter G., Griffin H. 1997; Characterisation of events during the late stages of HPV-16 infection using high affinity synthetic FAbs to E4. Virology 238:40–52
    [Google Scholar]
  7. Dyson N., Howley P., Munger K., Harlow E. 1989; The human papillomavirus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243:934–936
    [Google Scholar]
  8. Huang D., Cory S., Strasser A. 1997; Bcl-2, Bcl-XL and adenovirus protein E1B are functionally equivalent in their ability to inhibit cell death. Oncogene 14:405–414
    [Google Scholar]
  9. Huibregtse J., Scheffner M., Howley P. 1991; A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18. EMBO Journal 10:4129–4135
    [Google Scholar]
  10. Huibregtse J., Scheffner M., Howley P. 1993; Localisation of the E6-AP regions that direct human papillomavirus E6 binding, association with p53, and ubiquitination of associated proteins. Molecular and Cellular Biology 13:4918–4927
    [Google Scholar]
  11. Krajewski S., Krajewska M., Shabaik A., Miyashita T., Wang H., Reed J. 1994; Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2. American Journal of Pathology 145:1323–1336
    [Google Scholar]
  12. Krajewski S., Krajewska M., Reed J. 1996; Immunohistochemical analysis of in vivo patterns of Bak expression, a proapoptotic member of the Bcl-2 protein family. Cancer Research 56:2849–2855
    [Google Scholar]
  13. Morris J., Crook T., Bandara L., Davies R., LaThangue N., Vousden K. 1993; Human papillomavirus type 16 E7 regulates E2F and contributes to mitogenic signalling. Oncogene 8:893–898
    [Google Scholar]
  14. Nagata S. 1997; Apoptosis by death factor. Cell 88:355–365
    [Google Scholar]
  15. Pagano M., Durst M., Joswig S., Draetta G., Jansen-Dürr P. 1992; Binding of the human E2F transcription factor to the retinoblastoma protein but not to cyclin A is abolished in HPV-16-immortalised cells. Oncogene 7:1681–1686
    [Google Scholar]
  16. Pan H., Griep A. 1995; Temporally distinct patterns of p53-dependent and p53-independent apoptosis during mouse lens development. Genes & Development 9:2157–2169
    [Google Scholar]
  17. Phelps W., Bagchi S., Barnes J., Raychaudhuri P., Kraus V., Munger K., Howley P., Nevins J. 1991; Analysis oftransactivation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism. Journal of Virology 65:6922–6930
    [Google Scholar]
  18. Reed J. 1994; Bcl-2 and the regulation of programmed cell death. Journal of Cell Biology 124:1–6
    [Google Scholar]
  19. Scheffner M., Werness B., Huibregtse J., Levine A., Howley P. 1990; The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell 63:1129–1136
    [Google Scholar]
  20. Thomas M., Banks L. 1998; Inhibition of Bak-induced apoptosis by HPV-18 E6. Oncogene 17:2943–2954
    [Google Scholar]
  21. Werness B., Levine A., Howley P. 1990; Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science 248:76–79
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-6-1513
Loading
/content/journal/jgv/10.1099/0022-1317-80-6-1513
Loading

Data & Media loading...

Most cited Most Cited RSS feed