Epitopes on glycoprotein C of bovine herpesvirus-1 (BHV-1) that allow differentiation between BHV-1.1 and BHV-1.2 strains Free

Abstract

In cattle, bovine herpesvirus-1 (BHV-1) can cause a mild genital disease known as infectious pustular vulvovaginitis (IPV) and a more severe respiratory disease known as infectious bovine rhinotracheitis (IBR). On the basis of epidemiological data, it has been proposed that these diseases are caused by strains with different genotypes (IBR by BHV-1.1 and IPV by BHV-1.2 strains). By using a panel of 237 BHV-1 isolates, a monoclonal antibody (MAb 71) was found that failed to react with all 54 putative IPV strains in the panel, and another MAb (77) was found that did not react with 16 of these 54 IPV strains. Because MAbs 71 and 77 also failed to react with a BHV-1.1 glycoprotein C (gC)-deletion mutant, it was hypothesized that both MAbs recognize BHV-1.1 gC. By marker-rescue experiments and by expressing fragments of the BHV-1.1 gC gene in recombinant baculoviruses, it was shown that both MAbs indeed recognize BHV-1.1 gC. MAb 71 recognizes the N-terminal half and MAb 77 recognizes the C-terminal half of BHV-1.1 gC. In a PEPSCAN analysis with 12-mer oligopeptides, MAb 71 reacted with overlapping peptides containing gC amino acid residues 75-80 and MAb 77 did not react in this analysis. The differences in gC found in this study may contribute to the biological differences between BHV-1.1 and BHV-1.2.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-6-1477
1999-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/6/0801477a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-6-1477&mimeType=html&fmt=ahah

References

  1. Brake F., Studdert M. J. 1985; Molecular epidemiology and pathogenesis of ruminant herpesviruses including bovine, buffalo and caprine herpesviruses 1 and bovine encephalitis herpesvirus. Australian Veterinary Journal 62:331–334
    [Google Scholar]
  2. Christensen L. S., Madsen K. G., Nylin B., Ronsholt L. 1996; A contribution to the systematization of bovine herpesvirus 1 based on genomic mapping by restriction fragment pattern analysis. Virus Research 46:1770–182
    [Google Scholar]
  3. Edwards S., White H., Nixon P. 1990; A study of the predominant genotypes of bovid herpesvirus 1 found in the U.K. Veterinary Microbiology 22:213–223
    [Google Scholar]
  4. Edwards S., Newman R. H., White H. 1991; The virulence of British isolates of bovid herpesvirus 1 in relationship to viral genotype. British Veterinary Journal 147:216–231
    [Google Scholar]
  5. Engels M., Steck F., Wyler R. 1981; Comparison of the genomes of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis virus strains by restriction endonuclease analysis. Archives of Virology 67:169–174
    [Google Scholar]
  6. Fitzpatrick D. R., Babiuk L. A., Zamb T. J. 1989; Nucleotide sequence of bovine herpesvirus type 1 glycoprotein gIII, a structural model for gIII as a new member of the immunoglobulin superfamily, and implications for the homologous glycoproteins of other herpesviruses. Virology 173:46–57
    [Google Scholar]
  7. Geysen H. M., Meloen R. H., Barteling S. J. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, USA 81:3998–4002
    [Google Scholar]
  8. Gillespie J. H., McEntee H., Kendrick J. W., Wagner W. C. 1959; Comparison of infectious pustular vulvovaginitis virus with infectious bovine rhinotracheitis virus. Cornell Veterinarian 49:288–297
    [Google Scholar]
  9. Graham F. L., van der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  10. Greig A. S., Bannester C. L., Mitchell D., Barker C. A. V. 1958; Cultivation in tissue culture of an infectious agent from coital exanthema of cattle. Canadian Journal for Comparative Medicine 22:119–122
    [Google Scholar]
  11. Harlow E., Lane D. 1988 Antibodies, A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Hecht P., Engels M., Loepfe E., Ackermann M. 1995; Comparison of the glycoprotein gC genes of bovine and caprine herpesviruses. In Immunobiology of Viral Infections. Proceedings of the 3rd Congress of the European Society for Veterinary Virology pp 147–152 Edited by Schwyzer M., Ackermann M. Lyon: Fondation Marcel Mérieux;
    [Google Scholar]
  13. Kramps J. A., Magdalena J., Quak J., Weerdmeester K., Kaashoek M. J., Maris-Veldhuis M. A., Rijsewijk F. A. M., Keil G., van Oirschot J. T. 1994; A simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1. Journal of Clinical Microbiology 32:2175–2181
    [Google Scholar]
  14. Liang X., Babiuk L. A., Zamb T. J. 1993; Mapping of heparinbinding structures on bovine herpesvirus 1 and pseudorabies virus gIII glycoproteins. Virology 194:233–243
    [Google Scholar]
  15. Luckow V. A., Lee S. C., Barry G. F., Olins P. O. 1993; Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. Journal of Virology 67:4566–4579
    [Google Scholar]
  16. Ludwig H. 1984; Herpesviruses of bovidae: the characterization, grouping and role of different types, including latent viruses. In Latent Herpes Virus Infections in Veterinary Medicine pp 171–189 Edited by Wittmann G., Gaskell R. M., Rhiza H.-J. Zoetermeer: Martinus Nijhoff;
    [Google Scholar]
  17. Mayfield J. E., Good P. J., van Oort H. J., Campbell A. R., Reed D. E. 1983; Cloning and cleavage site mapping of DNA from bovine herpesvirus 1 (Cooper strain). Journal of Virology 47:259–264
    [Google Scholar]
  18. Metzler A. E., Matile H., Gassmann U., Engels M., Wyler R. 1985; European isolates of bovine herpesvirus 1: a comparison of restriction endonuclease sites, polypeptides, and reactivity with monoclonal antibodies. Archives of Virology 85:57–69
    [Google Scholar]
  19. Miller N. J. 1955; Infectious necrotic rhinotracheitis of cattle. Journal of the American Veterinary Medical Association 126:463–467
    [Google Scholar]
  20. Miller J. M., Whetstone C. A., Van der Maaten M. J. 1991; Abortifacient property of bovine herpesvirus type 1 isolates that represent three subtypes determined by restriction endonuclease analysis of viral DNA. American Journal for Veterinary Research 52:458–461
    [Google Scholar]
  21. Misra V., Babiuk L. A., Darcel C.le Q. 1983; Analysis of bovine herpes virus-type 1 isolates by restriction endonuclease fingerprinting. Archives of Virology 76:341–354
    [Google Scholar]
  22. Okazaki K., Honda E., Kono Y. 1994; Heparin-binding domain of bovidherpesvirus 1 glycoprotein gIII. Archives of Virology 134:413–419
    [Google Scholar]
  23. Pauli G., Gregersen J. P., Storz J., Ludwig H. 1984; Biology and molecular biology of latent bovine herpes virus type 1 (BHV-1). In Latent Herpes Virus Infections in Veterinary Medicine pp 229–239 Edited by Wittmann G., Gaskell R. M., Rhiza H. J. Zoetermeer: Martinus Nijhoff;
    [Google Scholar]
  24. Rola J., Żmudziński J. 1995; Analiza restrykcyjna wariantow fenotypowych wirusa BHV1. Medycyna Weterynaryjna 51:472–473 (in Polish)
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Spear P. G. 1993; Entry of alphaherpesviruses into cells. Seminars in Virology 4:167–180
    [Google Scholar]
  27. van Oirschot J. T., Rijsewijk F. A. M., Straver P. J., Ruuls R. C., Quak J., Davidse A., Westenbrink F., Gielkens A. L. J., van Dijk J. E., Moerman A. 1995; Virulence and genotype of a bovine herpesvirus 1 isolate from semen of a subclinically infected bull. Veterinary Record 137:235–239
    [Google Scholar]
  28. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  29. Von Grunder H. D., Reulaux I. R., Liess B. 1960; Feststellung der virusbedingten Rhinotracheitis infectiosa des Rindes. Deutsche Tierarztliche Wochenschrift 67:514–519 (in German)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-6-1477
Loading
/content/journal/jgv/10.1099/0022-1317-80-6-1477
Loading

Data & Media loading...

Most cited Most Cited RSS feed