RT Journal Article SR Electronic(1) A1 Singh, Dinesh K. A1 Chebloune, Yahia A1 Mselli-Lakhal, Leila A1 Karr, Bradley M. A1 Narayan, OpendraYR 1999 T1 Ovine lentivirus-infected macrophages mediate productive infection in cell types that are not susceptible to infection with cell-free virus JF Journal of General Virology, VO 80 IS 6 SP 1437 OP 1444 DO https://doi.org/10.1099/0022-1317-80-6-1437 PB Microbiology Society, SN 1465-2099, AB Ovine lentiviruses and caprine arthritis-encephalitis virus (CAEV) are prototypic lentiviruses that replicate predominantly in macrophages of infected animals. In situ hybridization of pathologically affected tissues from diseased animals has shown that viral RNA exists in permissive macrophages as well as in non-macrophage cell types that do not support productive virus replication. These findings raise questions about the cellular tropism of these viruses in vivo and how this may relate to their pathogenesis and the establishment of persistent infections. In this study, the susceptibility of macrophages and fibro-epithelial cells derived from goat synovial membrane (GSM) to infection by 14 North American ovine lentivirus strains was examined. All 14 strains were macrophage-tropic, as indicated by expression of viral proteins and by fusion and development of syncytial cytopathic effects following co-culture of infected macrophages with GSM cells. In contrast, neither viral DNA nor viral proteins was detected in GSM cells inoculated with cell-free virus from nine of the 14 strains. Specific virus proteins were immunoprecipitated from restrictive GSM cells following culture with infected macrophages and serial passage of GSM cells to remove the macrophages. The lack of infection of GSM cells by cell-free virus from some ovine lentivirus field strains was circumvented by cell-associated virus infection from infected macrophages to GSM cells following cell-to-cell contact. This strategy could be one of the mechanisms involved in the escape from immune surveillance and establishment of persistent infection in infected animals., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-80-6-1437