1887

Abstract

Respiratory syncytial virus (RSV)-specific cytotoxic T lymphocytes (CTL) or neutralizing antibodies can protect against RSV infection when induced separately by immunization with synthetic peptides. In the work described here, RSV-specific neutralizing antibodies and CTLs were induced after immunization with a cocktail of peptides consisting of a B-cell mimotope (S1S-MAP), a T-helper epitope (SH:45-60) and a CTL epitope linked to a fusion (F) peptide (F/M2:81-95) that were comparable to those induced by the peptides alone. Following challenge, a 190-fold reduction in RSV titre was observed in the lungs of peptide cocktail-immunized mice. The combination of RSV-specific humoral and cellular immunity induced by the peptide cocktail was thus more effective at clearing RSV than peptide-induced humoral or cellular immunity alone.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-6-1401
1999-06-01
2022-05-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/6/0801401a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-6-1401&mimeType=html&fmt=ahah

References

  1. Alwan W. H., Record F. M., Openshaw P. J. 1992; CD4 + T cells clear virus but augment disease in mice infected with respiratory syncytial virus. Comparison with the effects of CD8 + T cells. Clinical and Experimental Immunology 88:527–536
    [Google Scholar]
  2. Bachmann M. F., Kalinke U., Althage A., Freer G., Burkhart C., Roost H. P., Aguet M., Hengartner H., Zingernagel R. M. 1997; The role of antibody concentration and avidity in antiviral protection. Science 276:2024–2027
    [Google Scholar]
  3. Cannon M. J., Stott E. J., Taylor G., Askonas B. A. 1987; Clearance of persistent respiratory syncytial virus infections in immunodeficient mice following transfer of primed T cells. Immunology 62:133–138
    [Google Scholar]
  4. Chargelegue D., Obeid O. E., Hsu S. C., Shaw M. D., Denbury A. N., Taylor G., Steward M. W. 1998; A peptide mimic of a protective epitope of respiratory syncytial virus selected from a combinatorial library induces virus-neutralizing antibodies and reduces viral load in vivo. Journal of Virology 72:2040–2046
    [Google Scholar]
  5. Chin J., Magoffin R. L., Shearer L. A., Schieble J. H., Lennette E. H. 1969; Field evaluation of a respiratory syncytial virus vaccine and a trivalent parainfluenza virus vaccine in a pediatric population. American Journal of Epidemiology 89:449–463
    [Google Scholar]
  6. Connors M., Collins P. L., Firestone C. Y., Murphy B. R. 1991; Respiratory syncytial virus (RSV) F, G, M2 (22K), and N proteins each induce resistance to RSV challenge, but resistance induced by M2 and N proteins is relatively short-lived. Journal of Virology 65:1634–1637
    [Google Scholar]
  7. Connors M., Kulkarni A. B., Firestone C. Y., Holmes K. L., Morse H. C. III, Sotnikov A. V., Murphy B. R. 1992; Pulmonary histopathology induced by respiratory syncytial virus (RSV) challenge of formalin-inactivated RSV-immunized BALB/c mice is abrogated by depletion of CD4 + T cells. Journal of Virology 66:7444–7451
    [Google Scholar]
  8. Crowe J. E. Jr, Bui P. T., Davis A. R., Chanock R. M., Murphy B. R. 1994; A further attenuated derivative of a cold-passaged temperature-sensitive mutant of human respiratory syncytial virus retains immunogenicity and protective efficacy against wild-type challenge in seronegative chimpanzees. Vaccine 12:783–790
    [Google Scholar]
  9. Fulginiti V. A., Eller J. J., Sieber O. F., Joyner J. W., Minamitani M., Meiklejohn G. 1969; Respiratory virus immunization. I. A field trial of two inactivated respiratory virus vaccines; an aqueous trivalent parainfluenza virus vaccine and an alum-precipitated respiratory syncytial virus vaccine. American Journal of Epidemiology 89:435–448
    [Google Scholar]
  10. Gaddum R. M., Cook R. S., Wyld S. G., Lopéz J. A., Bustos R., Wyld S. G., Lopéz J. A., Bustos R., Melero J. A., Taylor G. 1996; Mutant forms of the F protein of human respiratory syncytial (RS) virus induce a cytotoxic T lymphocyte response but not a neutralizing antibody response and only transient resistance to RS virus infection. Journal of General Virology 77:1239–1248
    [Google Scholar]
  11. Graham B. S., Bunton L. A., Wright P. F., Karzon D. T. 1991; Role of T lymphocyte subsets in the pathogenesis of primary infection and rechallenge with respiratory syncytial virus in mice. Journal of Clinical Investigation 88:1026–1033
    [Google Scholar]
  12. Groothuis J. R., Simoes E. A., Levin M. J., Hall C. B., Long C. E., Rodriguez W. J., Arrobio J., Meissner H. C., Fulton D. R., Welliver R. C. and others 1993; Prophylactic administration of respiratory syncytial virus immune globulin to high-risk infants and young children. The Respiratory Syncytial Virus Immune Globulin Study Group. New England Journal of Medicine 329:1524–1530
    [Google Scholar]
  13. Hall C. B. 1994; Prospects for a respiratory syncytial virus vaccine. Science 265:1393–1394
    [Google Scholar]
  14. Heilman C. A. 1990; From the National Institute of Allergy and Infectious Diseases and the World Health Organization. Respiratory syncytial and parainfluenza viruses. Journal of Infectious Diseases 161:402–406
    [Google Scholar]
  15. Hsu S. C., Chargelegue D., Steward M. W. 1998; Reduction of respiratory syncytial virus titer in the lungs of mice after intranasal immunization with a chimeric peptide consisting of a single CTL epitope linked to a fusion peptide. Virology 240:376–381
    [Google Scholar]
  16. Kulkarni A. B., Collins P. L., Bacik I., Yewdell J. W., Bennink J. R., Crowe J. E. Jr, Murphy B. R. 1995; Cytotoxic T cells specific for a single peptide on the M2 protein of respiratory syncytial virus are the sole mediators of resistance induced by immunization with M2 encoded by a recombinant vaccinia virus. Journal of Virology 69:1261–1264
    [Google Scholar]
  17. Openshaw P. J., Clarke S. L., Record F. M. 1992; Pulmonary eosinophilic response to respiratory syncytial virus infection in mice sensitized to the major surface glycoprotein G. International Immunology 4:493–500
    [Google Scholar]
  18. Prince G. A., Hemming V. G., Horswood R. L., Chanock R. M. 1985; Immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. Virus Research 3:193–206
    [Google Scholar]
  19. Rodriguez F., Zhang J., Whitton J. L. 1997; DNA immunization: ubiquitination of a viral protein enhances cytotoxic T-lymphocyte induction and antiviral protection but abrogates antibody induction. Journal of Virology 71:8497–8503
    [Google Scholar]
  20. Sparer T. E., Matthews S., Hussell T., Rae A. J., Garcia-Barreno B., Melero J. A., Openshaw P. J. 1998; Eliminating a region of respiratory syncytial virus attachment protein allows induction of protective immunity without vaccine-enhanced lung eosinophilia. Journal of Experimental Medicine 187:1921–1926
    [Google Scholar]
  21. Speidel K., Osen W., Faath S., Hilgert I., Obst R., Braspenning J., Momburg F., Hammerling G. J., Rammensee H. G. 1997; Priming of cytotoxic T lymphocytes by five heat-aggregated antigens in vivo: conditions, efficiency, and relation to antibody responses. European Journal of Immunology 27:2391–2399
    [Google Scholar]
  22. Srikiatkhachorn A., Braciale T. J. 1997; Virus-specific CD8 + T lymphocytes downregulate T helper cell type 2 cytokine secretion and pulmonary eosinophilia during experimental murine respiratory syncytial virus infection. Journal of Experimental Medicine 186:421–432
    [Google Scholar]
  23. Trudel M., Nadon F., Seguin C., Brault S., Lusignan Y., Lemieux S. 1992; Initiation of cytotoxic T-cell response and protection of Balb/c mice by vaccination with an experimental ISCOMs respiratory syncytial virus subunit vaccine. Vaccine 10:107–112
    [Google Scholar]
  24. Walsh E.E. 1994; Humoral, mucosal, and cellular immune response to topical immunization with a subunit respiratory syncytial virus vaccine. Journal of Infectious Diseases 170:345–350
    [Google Scholar]
  25. Wertz G. W., Sullender M. W. 1992; Approaches to immunization against respiratory syncytial virus. In Vaccines: New Approaches to Immunological Problems pp 151–176 Edited by Eltis R. W. Boston: Butterworth-Heinemann;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-6-1401
Loading
/content/journal/jgv/10.1099/0022-1317-80-6-1401
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error