1887

Journal of General Virology header logo

Volume 80, Issue 5

Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1 Free

Abstract

To determine the potential of replication-competent (E3-deleted) bovine adenovirus-3 (BAV-3) as a delivery system for vaccine antigens in calves, we evaluated the ability of recombinant BAV-3 expressing different forms of of bovine herpesvirus-1 (BHV-1) glycoprotein gD to protect against BHV-1 infection in calves that had pre-existing BAV-3 specific antibodies. Three- to four-month-old calves, vaccinated intranasally with recombinant BAV-3 expressing full-length gD (BAV3.E3gD) or a truncated version of gD (gDt) (BAV3.E3gDt), or with E3-deleted BAV-3 (BAV3.E3d; control), were challenged with BHV-1 strain 108. Vaccination with BAV3.E3gD or BAV3.E3gDt induced gD-specific antibody responses in serum and nasal secretions, and primed calves for gD-specific lymphoproliferative responses. In addition, all calves developed complement-independent neutralizing antibodies against BHV-1. Protection against viral challenge was observed in calves vaccinated with recombinant BAV3.E3gD or BAV3.E3gDt as shown by a significant reduction in body temperature and clinical disease, and a partial reduction in the amount and duration of virus excretion in nasal secretions. These results indicate that replication-competent BAV-3-based vectors can induce protective immune responses in calves (the natural host) that have pre-existing BAV-3-specific antibodies.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1999
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-5-1263
1999-05-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/5/0801263a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-5-1263&mimeType=html&fmt=ahah

References

  1. Babiuk L. A., L’ltalien J., van Drunen Littel-van den Hurk S., Zamb T., Lawman M. J. P., Hughes G., Gifford G. A. 1987; Protection of cattle from bovine herpesvirus type-1 (BHV-1) infection by immunization with individual viral glycoproteins. Virology 159:57–66
     [Google Scholar]
  2. Babuik L. A., van Drunen Littel-van den Hurk S., Tikoo S. K., Lewis P. J., Liang X. 1996; Novel viral vaccines for livestock. Veterinary Immunology and Immunopathology 54:355–363
     [Google Scholar]
  3. Bartha A. 1969; Proposal for subgrouping of bovine adenoviruses. Acta Veterinaria Academiae Scientiarum Hungaricae 19:319–321
     [Google Scholar]
  4. Callebaut P., Enjuanes L., Pensaert M. 1996; An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine. Journal of General Virology 77:309–313
     [Google Scholar]
  5. Czerkinsky C. C., Nilsson L. A., Nygren H., Ouchterlony O., Tarkowski A. 1983; A solid phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. Journal of Immunological Methods 65:109
     [Google Scholar]
  6. Darbyshire J. H. 1968; Bovine adenoviruses. Journal of the American Medical Association 152:786–794
     [Google Scholar]
  7. Ertl H. C. J., Xiang Z. 1996; Novel vaccine approaches. Journal of Immunology 156:3579–3582
     [Google Scholar]
  8. Graham F. L., Prevec L. 1991; Manipulation of adenovirus vectors. Methods in Molecular Biology 7:363–390
     [Google Scholar]
  9. Imler J. L. 1995; Adenovirus vectors as recombinant viral vaccines. Vaccine 13:1143–1151
     [Google Scholar]
  10. Israel B. A., Herber R., Gao Y., Letchworth G. J. III 1992; Induction of a mucosal barrier to bovine herpesvirus 1 replication in cattle. Virology 188:256–264
     [Google Scholar]
  11. Mittal S. K., Prevec L., Graham F. L., Babiuk L. A. 1995; Development of bovine adenovirus type 3-based expression vector. Journal of General Virology 76:93–102
     [Google Scholar]
  12. Mittal S. K., Papp Z., Tikoo S. K., Baca-Estrada M. E., Yoo D., Benko M., Babiuk L. A. 1996; Induction of systemic and mucosal immune responses in cotton rats immunized with human adenovirus type 5 recombinants expressing the full and truncated forms of bovine herpesvirus type 1 glycoprotein gD. Virology 222:299–309
     [Google Scholar]
  13. Papp Z., Middleton D. M., Mittal S. K., Babiuk L. A., Baca-Estrada M. E. 1997; Mucosal immunization with recombinant adenoviruses: induction of immunity and protection of cotton rats against respiratory bovine herpesvirus type 1 infection. Journal of General Virology 78:2933–2943
     [Google Scholar]
  14. Papp Z., Babiuk L. A., Baca-Estrada M. E. 1998; Induction of immunity in respiratory tract and protection from bovine herpesvirus type 1 infection by different routes of immunization with recombinant adenovirus. Viral Immunology 11:79–91
     [Google Scholar]
  15. Prevec L., Schneider M., Rosenthal K. L., Belbeck L. W., Derbyshire J. B., Graham F. L. 1989; Use of human adenovirus-based vectors for antigen expression in animals. JournalofGeneral Virology 70:429–434
     [Google Scholar]
  16. Reddy P. S., Idamakanti N., Zakhartchouk A. N., Baxi M. K., Lee J. B., Pyne C., Babiuk L. A., Tikoo S. K. 1998; genome organization and the transcription map of bovine adenovirus type 3. Journal of Virology 72:1394–1402
     [Google Scholar]
  17. Rosenthal K. L., Copeland K. F. T., Gallichan W. S. 1996; Recombinant adenoviruses as vectors for mucosal immunity. In Mucosal Vaccines pp 147–158 Edited by Klyono H., Ogra P. L., McGhee J. R. San Diego: Academic Press;
     [Google Scholar]
  18. Rouse B. T., Babiuk L. A. 1974; Host responses to infectious bovine rhinotracheitis. III. Isolation and immunological activities of bovine lymphocytes. Journal of Immunology 113:1391–1398
     [Google Scholar]
  19. Tikoo S. K., Fitzpatrick D., Babiuk L. A., Zamb T. J. 1990; Molecular cloning, sequencing and expression of functional bovine herpesvirus-1 glycoprotein gIV in transfected bovine cells. Journal of Virology 64:5132–5142
     [Google Scholar]
  20. Tikoo S. K., Zamb T. J., Babiuk L. A. 1993; Analysis of bovine herpesvirus-1 glycoprotein gIV truncations and deletions expressed by recombinant vaccinia viruses. Journal of Virology 67:2103–2109
     [Google Scholar]
  21. Torres J. M., Alonso C., Ortega A., Mittal S., Graham F., Enjuanes L. 1996; Tropism of human adenovirus type 5-based vectors in swine and their ability to protect against transmissible gastroenteritis coronavirus. Journal of Virology 70:3770–3780
     [Google Scholar]
  22. Van Drunen Littel-van den Hurk S., Parker M. D., Massie B., van den Hurk J., Harland R., Babiuk L. A., Zamb T. J. 1993; Protection of cattle from BHV-1 infection by immunization with recombinant glycoprotein gIV. Vaccine 11:25–35
     [Google Scholar]
  23. Van Drunen Littel-van den Hurk S., van Donkersgoed J., Kowalski J., van den Hurk J. V., Harland R., Babiuk L. A., Zamb T. J. 1994; A subunit gIV vaccine produced by transfected mammalian cells in culture, induces mucosal immunity against bovine herpesvirus-1 in cattle. Vaccine 12:1295–1302
     [Google Scholar]
  24. Van Drunen Littel-van den Hurk S., Tikoo S. K., van den Hurk J. V., Babiuk L. A., van Donkersgoed J. 1997; Protective immunity in cattle following vaccination with conventional and marker bovine herpesvirus-1 (BHV-1) vaccines. Vaccine 15:36–44
     [Google Scholar]
  25. Yates W. D. G. 1982; A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral bacterial synergism in respiratory diseases of cattle. Canadian Journal of Comparative Medicine 46:225–263
     [Google Scholar]
  26. Zakhartchouk A. N., Reddy P. S., Baxi M., Baca-Estrada M. E., Mehtali M., Babiuk L. A., Tikoo S. K. 1998; Construction and characterization of E3-deleted bovine adenovirus type 3 expressing full-length and truncated form of bovine herpesvirus type 1 glycoprotein gD. Virology 250:220–229
     [Google Scholar]
  27. Zu X., Letchworth G. J. 1996; Mucosal and systemic immunity to bovine herpesvirus-1 glycoprotein D confer resistance to viral replication and latency in cattle. Vaccine 14:61–69
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-5-1263
Loading
Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1
J. Gen. Virol. 80, 1263 (1999); https://doi.org/10.1099/0022-1317-80-5-1263
/content/journal/jgv/10.1099/0022-1317-80-5-1263
/content/journal/jgv/10.1099/0022-1317-80-5-1263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error