1887

Abstract

Virions of lettuce infectious yellows virus (LIYV; genus ) were purified from LIYV-infected plants and their protein composition was analysed by SDS-PAGE and immunoblotting. Virion preparations contained the major capsid protein (CP), but the minor capsid protein (CPm), p59 and the HSP70 homologue were also identified by immunoblot analysis. Immunogold labelling analysis showed that CP constituted the majority of the LIYV virion capsid, but CPm was also part of the capsid and localized to one end of the virion, similar to the polar morphology seen for viruses in the genus . p59 and the HSP70 homologue were not detected on virions by immunogold labelling, but were always detected in virion preparations by immunoblot analysis. Purified LIYV virions were used for acquisition analysis with whiteflies and were efficiently transmitted to plants. Infectivity neutralization analyses were done using antisera to the LIYV-encoded CP, CPm, p59 and HSP70 homologue. Only antiserum to the CPm effectively neutralized LIYV transmission by . . These data suggest that the LIYV-. transmission determinants are associated with purified virions, and that the LIYV virion structural protein CPm is involved in transmission by B. tobaci.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-5-1111
1999-05-01
2024-11-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/5/0801111a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-5-1111&mimeType=html&fmt=ahah

References

  1. Agranovsky A. A. 1996; Principles of molecular organization, expression, and evolution of closteroviruses: over the barriers. Advances in Virus Research 47:119–158
    [Google Scholar]
  2. Agranovsky A. A., Koonin E. V., Boyko V. P., Maiss E., Frotschl R., Lunina N. A., Atabekov J. G. 1994; Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease. Virology 198:311–324
    [Google Scholar]
  3. Agranovsky A. A., Lesemann D. E., Maiss E., Hull R., Atabekov J. G. 1995; ‘Rattlesnake’ structure of a filamentous plant RNA virus built of two capsid proteins. Proceedings of the National Academy of Sciences, USA 92:2470–2473
    [Google Scholar]
  4. Bandla M. D., Campbell L. R., Ullman D. E., Sherwood J. L. 1998; Interaction of tomato spotted wilt tospovirus (TSWV) glycoproteins with a thrips midgut protein, a potential cellular receptor for TSWV. Phytopathology 88:98–104
    [Google Scholar]
  5. Bruyere A., Brault V., Ziegler-Graff V., Simonis M. T., Van den Heuvel J. F., Richards K., Guilley H., Jonard G., Herrbach E. 1997; Effects of mutations in the beet western yellows virus readthrough protein on its expression and packaging and on virus accumulation, symptoms, and aphid transmission. Virology 230:323–334
    [Google Scholar]
  6. Dolja V. V., Karasev A. V., Koonin E. V. 1994; Molecular biology and evolution of closteroviruses: sophisticated build-up of large RNA genomes. Annual Review of Phytopathology 32:261–285
    [Google Scholar]
  7. Duffus J. E. 1990; Infectivity neutralization. In Serological Methods for Detection and Identification of Viral and Bacterial Plant Pathogens pp 339–341 Edited by Hampton R., Ball E., DeBoer S. St Paul, MN: APS Press;
    [Google Scholar]
  8. Duffus J. E., Larsen R. C., Liu H. Y. 1986; Lettuce infectious yellows virus –a new type of whitefly-transmitted virus. Phytopathology 76:97–100
    [Google Scholar]
  9. Duffus J.E., Liu H.Y., Wisler G.C., Li R.H. 1996; Lettuce chlorosis virus –a new whitefly-transmitted closterovirus. European Journal of Plant Pathology 102:591–596
    [Google Scholar]
  10. Elnagar S., Murant A. F. 1976; The role of the helper virus, anthriscus yellows, in the transmission of parsnip yellow fleck virus by the aphid Cavariella aegopodii . Annals of Applied Biology 84:169–181
    [Google Scholar]
  11. Febres V. J., Ashoulin L., Mawassi M., Frank A., Bar-Joseph M., Manjunath K. L., Lee R. F., Niblett C. L. 1996; The p27 protein is present at one end of citrus tristeza virus particles. Phytopathology 86:1331–1335
    [Google Scholar]
  12. Harrison B. D., Murant A. F. 1984; Involvement of virus-coded proteins in transmission of plant viruses by vectors. In Vectors in Virus Biology pp 1–36 Edited by Mayo M. A., Harrap K. A. London: Academic Press;
    [Google Scholar]
  13. He X., Harper K., Grantham G., Yang C. H., Creamer R. 1998; Serological characterization of the 3′ -proximal encoded proteins of beet yellows closterovirus. Archives of Virology 143:1349–1363
    [Google Scholar]
  14. Hofer P., Bedford I. D., Markham P. G., Jeske H., Frischmuth T. 1997; Coat protein gene replacement results in whitefly transmission of an insect nontransmissible geminivirus isolate. Virology 236:288–295
    [Google Scholar]
  15. Karasev A. V., Boyko V. P., Gowda S., Nikolaeva O. V., Hilf M. E., Koonin E. V., Niblett C. L., Cline K. C., Gumpf D. J., Lee R. F., Garnsey S. M., Dawson W. O. 1995; Complete sequence of the citrus tristeza virus RNA genome. Virology 208:511–520
    [Google Scholar]
  16. Kikkert M., Meurs C., Van De Wetering F., Dorfmuller S., Peters D., Kormelink R., Goldbach R. 1998; Binding of tomato spotted wilt virus to a 94 kDa thrips protein. Phytopathology 88:63–69
    [Google Scholar]
  17. Klaassen V. A., Boeshore M. E., Dolja V. V., Falk B. W. 1994; Partial characterization of the lettuce infectious yellows virus genomic RNAs, identification of the coat protein gene and comparison of its amino acid sequence with those of other filamentous RNA plant viruses. Journal of General Virology 75:1525–1533
    [Google Scholar]
  18. Klaassen V. A., Boeshore M., Koonin E. V., Tian T., Falk B. W. 1995; Genome structure and phylogenetic analysis of lettuce infectious yellows virus, a whitefly transmitted, bipartite closterovirus. Virology 208:99–110
    [Google Scholar]
  19. Klaassen V. A., Mayhew D., Fisher D., Falk B. W. 1996; In vitro transcripts from cloned cDNAs of the lettuce infectious yellows closterovirus bipartite genomic RNAs are competent for replication in Nicotiana benthamiana protoplasts. Virology 222:169–175
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  21. Manjunath K. L. 1985; Studies on the distribution, transmission, strains and strain interaction of citrus tristeza virus. Journal of Plant Disease Protection 92:502–508
    [Google Scholar]
  22. Medina V., Tian T., Wierzchos J., Falk B. W. 1998; Specific inclusion bodies are associated with replication of lettuce infectious yellows virus RNAs in Nicotiana benthamiana protoplasts. Journal of General Virology 78:2325–2329
    [Google Scholar]
  23. Nault L. R. 1997; Arthropod transmission of plant viruses: a new synthesis. Annals of the Entomological Society of America 90:521–541
    [Google Scholar]
  24. Peng Y. H., Kadoury D., Gal-On A., Huet H., Wang Y., Raccah B. 1998; Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions. Journal of General Virology 79:897–904
    [Google Scholar]
  25. Perring T. M., Cooper A. D., Rodriguez R. J., Farrar C. A., Bellows T. S. Jr 1993; Identification of a whitefly species by genomic and behavioral studies. Science 259:74–77
    [Google Scholar]
  26. Perry K. L., Zhang L., Palukaitis P. 1998; Amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the aphids Myzus persicae and Aphis gossypii . Virology 242:204–210
    [Google Scholar]
  27. Pirone T. P. 1991; Viral genes and gene products that determine insect transmissibility. Seminars in Virology 2:81–87
    [Google Scholar]
  28. Pirone T. P., Blanc S. 1996; Helper-dependent vector transmission of plant viruses. Annual Review of Phytopathology 34:227–247
    [Google Scholar]
  29. Rochow W. F., Duffus J. E. 1978; Relationships between barley yellow dwarf and beet western yellows viruses [serology, infectivity, transmission by aphids]. Phytopathology 68:51–58
    [Google Scholar]
  30. Schmidt I., Blanc S., Esperandieu P., Kuhl G., Devauchelle G., Louis C., Cerutti M. 1994; Interaction between the aphid transmission factor and virus particles is a part of the molecular mechanism of cauliflower mosaic virus aphid transmission. Proceedings of the National Academy of Sciences, USA 91:8885–8889
    [Google Scholar]
  31. Tian T., Medina V., Mayhew D. E., Maeda S., Falk B. W. 1995; Beet western yellows luteovirus capsid proteins produced by recombinant baculoviruses assemble into virion-like particles in cells and larvae of Bombyx mori . Virology 213:204–212
    [Google Scholar]
  32. Tian T., Klaassen V. A., Soong J., Wisler G., Duffus J. E., Falk B.W. 1996; Generation of cDNAs specific to lettuce infectious yellows closterovirus and other whitefly-transmitted viruses by RT–PCR and degenerate oligonucleotide primers to the closterovirus gene encoding the HSP70 homologue. Phytopathology 86:1167–1173
    [Google Scholar]
  33. Wang R. Y., Powell G., Hardie J., Pirone T. P. 1998; Role of the helper component in vector-specific transmission of potyviruses. Journal of General Virology 79:1519–1524
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-80-5-1111
Loading
/content/journal/jgv/10.1099/0022-1317-80-5-1111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error