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Volume 80, Issue 4

Human cytomegalovirus IE1 protein activates AP-1 through a cellular protein kinase(s). Free

Abstract

During human cytomegalovirus (HCMV) infection, a rapid increase in AP-1 activity is detected. In this study, activation of transcription from promoters containing AP-1-binding sites by the IE1 protein of HCMV was examined. In transient transfection assays with reporter plasmids, it was found that IE1 strongly induced AP-1-driven transcription. Cells stably expressing IE1 also showed higher levels of AP-1 activity than did control cells. IE1 expression did not raise levels of c-jun and c-fos RNA, as determined by quantitative RT-PCR. AP-1 induction by IE1 was blocked efficiently by protein kinase inhibitors in a cell type-dependent manner; for example, by staurosporine in the human microglial cell line U373MG and by H7 in the human promonocytic cell line U937. IE1-driven activation of AP-1 was increased dramatically by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 1 (MEKK1). The results of this study indicate that IE1 activates AP-1 at the post-transcriptional level and that MEKK1 may play an important role in this process.

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© Journal of General Virology 1999
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1999-04-01
2023-09-25
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Human cytomegalovirus IE1 protein activates AP-1 through a cellular protein kinase(s).
J. Gen. Virol. 80, 961 (1999); https://doi.org/10.1099/0022-1317-80-4-961
/content/journal/jgv/10.1099/0022-1317-80-4-961
/content/journal/jgv/10.1099/0022-1317-80-4-961
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