A Sabin vaccine-derived field isolate of poliovirus type 1 displaying aberrant phenotypic and genetic features, including a deletion in antigenic site 1. Free

Abstract

Poliovirus strains derived from the oral poliovirus vaccine (Sabin) can be differentiated from wild-type poliovirus by tests based on either immunological or genetic properties of the strains. The characterization of a recently identified poliovirus type 1 isolate with exceptional properties is described. Initial phenotypic analysis of the virus by use of polyclonal absorbed antisera suggested a wild-type character. However, the different genomic analyses all confirmed the Sabin-derived character of the virus. All 17 plaques isolated from the strain shared these properties, thus excluding the possibility of a mixture of a wild-type and a Sabin-derived strain. To elucidate the properties of this virus further, the nucleotide sequences of the P1 region and most of the 5′ non-coding region were established. Although the nucleotide identity with Sabin 1 was more than 99.4%, mutations were observed in regions encoding three major antigenic sites; the deduced amino acid substitutions confirmed the aberrant results of micro-neutralization assays with site-specific monoclonal antibodies. The most striking feature was the existence of a hexanucleotide deletion in the VP1 gene, which gave rise to a two amino acid deletion in the BC loop. In spite of these antigenic changes, the strain was readily serotyped as poliovirus type 1 under standard conditions. Likewise, replication of the virus under cell culture conditions was not affected by these mutations or by the deletion. Standard polio vaccination protects against this aberrant virus, and its epidemiological significance remains open.

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1999-04-01
2024-03-28
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References

  1. Agol V. I. 1997; Recombination and other genomic rearrangements in picornaviruses. Seminars in Virology 8:77–84
    [Google Scholar]
  2. Balanant J., Guillot S., Candrea A., Delpeyroux F., Crainic R. 1991; The natural genomic variability of poliovirus analyzed by a restriction fragment length polymorphism assay. Virology 184:645–654
    [Google Scholar]
  3. Bouchard M. J., Lam D. H., Racaniello V. R. 1995; Determinants of attenuation and temperature sensitivity in the type 1 poliovirus Sabin vaccine. Journal of Virology 69:4972–4978
    [Google Scholar]
  4. Cammack N., Phillips A., Dunn G., Patel V., Minor P. D. 1988; Intertypic genomic rearrangements of poliovirus strains in vaccinees. Virology 167:507–514
    [Google Scholar]
  5. CDC 1997; Paralytic poliomyelitis - United States, 1980-1994. Morbidity and Mortality Weekly Report 46:79–83
    [Google Scholar]
  6. Couderc T., Martin A., Wychowski C., Girard M., Horaud F., Crainic R. 1991; Analysis of neutralization-escape mutants selected from a mouse virulent type 1 /type 2 chimeric poliovirus: identification of a type 1 poliovirus with antigenic site 1 deleted. Journal of General Virology 72:973–977
    [Google Scholar]
  7. De L., Nottay B., Yang C. F., Holloway B. P., Pallansch M. A., Kew O. M. 1995; Identification of vaccine-related polioviruses by hybridization with specific RNA probes. Journal of Clinical Microbiology 33:562–571
    [Google Scholar]
  8. Domingo E., Escarmis C., Sevilla N., Moya A., Elena S. F., Quer J., Novella I. S., Holland J. J. 1996; Basic concepts in RNA virus evolution. FASEB Journal 10:859–864
    [Google Scholar]
  9. Dunn G., Begg N. T., Cammack N., Minor P. D. 1990; Virus excretion and mutation by infants following primary vaccination with live oral poliovaccine from two sources. Journal of Medical Virology 32:92–95
    [Google Scholar]
  10. Ferguson M., Magrath D. I., Minor P. D., Schild G. C. 1986; WHO collaborative study on the use of monoclonal antibodies for the intratypic differentiation of poliovirus strains. Bulletin of the World Health Organization 64:239–246
    [Google Scholar]
  11. Friedrich F., da Silva E. F., Schatzmayr H. G. 1996; Type 2 poliovirus recombinants isolated from vaccine-associated cases and from healthy contacts in Brazil. Acta Virologica 40:27–33
    [Google Scholar]
  12. Furione M., Guillot S., Otelea D., Balanant J., Candrea A., Crainic R. 1993; Polioviruses with natural recombinant genomes isolated from vaccine-associated paralytic poliomyelitis. Virology 196:199–208
    [Google Scholar]
  13. Georgescu M. M., Delpeyroux F., Tardy-Panit M., Balanant J., Combiescu M., Combiescu A. A., Guillot S., Crainic R. 1994; High diversity of poliovirus strains isolated from the central nervous system from patients with vaccine-associated paralytic poliomyelitis. Journal of Virology 68:8089–8101
    [Google Scholar]
  14. Georgescu M. M., Balanant J., Macadam A., Otelea D., Combiescu M., Combiescu A. A., Crainic R., Delpeyroux F. 1997; Evolution of the Sabin type 1 poliovirus in humans: characterization of strains isolated from patients with vaccine-associated paralytic poliomyelitis. Journal of Virology 71:7758–7768
    [Google Scholar]
  15. Hayward J. C., Gillespie S. M., Kaplan K. M., Packer R., Pallansch M., Plotkin S., Schonberger L. B. 1989; Outbreak of poliomyelitislike paralysis associated with enterovirus 71. Pediatric Infectious Disease Journal 8:611–616
    [Google Scholar]
  16. Holland J. J., de la Torre J. C., Steinhauer D. A. 1992; RNA virus populations as quasispecies. Current Topics in Microbiology Immunology 176:1–20
    [Google Scholar]
  17. Huovilainen A., Kinnunen L., Ferguson M., Hovi T. 1988; Antigenic variation among 173 strains of type 3 poliovirus isolated in Finland during the 1984 to 1985 outbreak. Journal of General Virology 69:1941–1948
    [Google Scholar]
  18. Johnson V. H., Semler B. L. 1988; Defined recombinants of poliovirus and coxsackievirus: sequence-specific deletions and functional substitutions in the 57-noncoding regions of viral RNAs. Virology 162:47–57
    [Google Scholar]
  19. Kew O. M., Mulders M. N., Lipskaya G. Y., da Silva E. E., Pallansch M. A. 1995; Molecular epidemiology of polioviruses. Seminars in Virology 6:401–414
    [Google Scholar]
  20. King A. M. 1988; Preferred sites of recombination in poliovirus RNA: an analysis of 40 intertypic cross-over sequences. Nucleic Acids Research 16:11705–11723
    [Google Scholar]
  21. Lapinleimu K. 1984; Elimination of poliomyelitis in Finland. Reviews Of Infectious Diseases 6:S457–S460
    [Google Scholar]
  22. Lipskaya G. Y., Muzychenko A. R., Kutitova O. K., Maslova S. V., Equestre M., Drozdov S. G., Bercoff R. P., Agol V. I. 1991; Frequent isolation of intertypic poliovirus recombinants with serotype 2 specificity from vaccine-associated polio cases. Journal ofMedical Virology 35:290–296
    [Google Scholar]
  23. Macadam A., Arnold C., Howlett J., John A., Marsden S., Taffs F., Reeve P., Hamada N., Wareham K., Almond J., Cammack N., Minor P. D. 1989; Reversion of the attenuated and temperature- sensitive phenotypes of the Sabin type 3 strain of poliovirus in vaccinees. Virology 172:408–414
    [Google Scholar]
  24. Melnick J. L. 1984; Enterovirus type 71 infections: a varied clinical pattern sometimes mimicking paralytic poliomyelitis. Reviews of Infectious Diseases 6:S387–S390
    [Google Scholar]
  25. Melnick J. L. 1996; Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses.. In Fields Virology 3rd edn pp 655–712 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott-Raven;
    [Google Scholar]
  26. Mendelsohn C., Johnson B., Lionetti K. A., Nobis P., Wimmer E., Racaniello V. R. 1986; Transformation of a human poliovirus receptor gene into mouse cells. Proceedings of the National Academy of Sciences, USA 83:7845–7849
    [Google Scholar]
  27. Minor P. D. 1990; Antigenic structure of picornaviruses. Current Topics in Microbiology Immunology 161:121–154
    [Google Scholar]
  28. Minor P. D. 1992; The molecular biology of poliovaccines. Journal of General Virology 73:3065–3077
    [Google Scholar]
  29. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  30. Mulders M. N., Koopmans M. P. G., van der Avoort H. G. A. M., van Loon A. M. 1995a; Detection and characterization of poliovirus - the molecular approach. In PCR: Protocol for Diagnosis of Human and Animal Virus Diseases. Frontiers of Virology pp 137–156 Edited by Becker Y., Darai G. Berlin: Springer–Verlag;
    [Google Scholar]
  31. Mulders M. N., Lipskaya G. Y., van der Avoort H. G., Koopmans M. P., Kew O. M., van Loon A. M. 1995b; Molecular epidemiology of wild poliovirus type 1 in Europe, the Middle East, and the Indian subcontinent. Journal of Infectious Diseases 171:1399–1405
    [Google Scholar]
  32. Nomoto A., Omata T., Toyoda H., Kuge S., Horie H., Kataoka Y., Genba Y., Nakano Y., Imura N. 1982; Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome. Proceedings of the National Academy of Sciences, USA 79:5793–5797
    [Google Scholar]
  33. Osterhaus A. D. M. E., van Wezel A. L., Hazendonk A. G., UytdeHaag F. C. G. M., van Asten J. A. A. M., van Steenis G. 1983; Monoclonal antibodies to polioviruses. Comparison of intratypic strain differentiation of poliovirus type 1 using monoclonal antibodies versus cross-absorbed antisera. Intervirology 20:129–136
    [Google Scholar]
  34. Pipkin P. A., Wood D. J., Racaniello V. R., Minor P. D. 1993; Characterisation of L cells expressing the human poliovirus receptor for the specific detection of polioviruses in vitro. Journal of Virological Methods 41:333–340
    [Google Scholar]
  35. Pulli T., Koskimies P., Hyypia T. 1995; Molecular comparison of coxsackie A virus serotypes. Virology 212:30–38
    [Google Scholar]
  36. Rico-Hesse R., Pallansch M. A., Nottay B. K., Kew O. M. 1987; Geographic distribution of wild poliovirus type 1 genotypes. Virology 160:311–322
    [Google Scholar]
  37. Rotbart H. A. 1990; Enzymatic RNA amplification of the enteroviruses. Journal of Clinical Microbiology 28:438–442
    [Google Scholar]
  38. Sabin A. B., Boulger L. R. 1973; History of Sabin attenuated poliovirus oral live vaccine strains. Journal of Biological Standardization 1:115–118
    [Google Scholar]
  39. Skinner M. A., Racaniello V. R., Dunn G., Cooper J., Minor P. D., Almond J. W. 1989; New model for the secondary structure of the 5 non-coding RNA of poliovirus is supported by biochemical and genetic data that also show that RNA secondary structure is important in neurovirulence. Journal of Molecular Biology 207:379–392
    [Google Scholar]
  40. Van der Avoort H. G. A. M., Hull B. P., Hovi T., Pallansch M. A., Kew O. M., Crainic R., Wood D. J., Mulders M. N., van Loon A. M. 1995; Comparative study of five methods for intratypic differentiation of polioviruses. Journal of Clinical Microbiology 33:2562–2566
    [Google Scholar]
  41. Van Wezel A. L., Hazendonk A. G. 1979; Intratypic sero- differentiation of poliomyelitis virus strains by strain-specific antisera. Intervirology 11:2–8
    [Google Scholar]
  42. WHO 1992 Manual for the Virological Investigation of Poliomyelitis Geneva: WHO;
    [Google Scholar]
  43. Yang C. F., De L., Holloway B. P., Pallansch M. A., Kew O. M. 1991; Detection and identification of vaccine-related polioviruses by the polymerase chain reaction. Virus Research 20:159–179
    [Google Scholar]
  44. Zheng D. P., Zhang L. B., Fang Z. Y., Yang C. F., Mulders M., Pallansch M. A., Kew O. M. 1993; Distribution of wild type 1 poliovirus genotypes in China. Journal of Infectious Diseases 1681361–1367
    [Google Scholar]
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