1887

Abstract

Binding sites for transcriptional regulation by the Myb protein have been identified in the long terminal repeats (LTRs) of the H-type human endogenous retrovirus-like elements (HERV-H). Transfection assays using reporter plasmids containing the luciferase gene under the control of a HERV-H LTR disclosed a sevenfold increase in promoter activity in human teratocarcinoma cells when cotransfected with an expression vector for the Myb protein. Binding sites for Myb were unambiguously identified within the LTR by both DNase I footprinting experiments and mobility shift assays using a bacterially expressed purified Myb recombinant protein. Possible roles of these Myb-responsive elements are discussed.

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1999-04-01
2022-05-28
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References

  1. Anderssen S., Sjottem E., Svineng G., Johansen T. 1997; Comparative analyses of LTRs of the ERV-H family of primate-specific retrovirus-like elements isolated from marmoset, African green monkey, and man. Virology 234:14–30
    [Google Scholar]
  2. Bosselut R., Lim F., Romond P.-C., Frampton J., Brady J., Ghysdael J. 1992; Myb protein binds to multiple sites in the human T-cell lymphotropic virus type 1 long terminal repeat and transactivates LTR-mediated expression. Virology 186:764–769
    [Google Scholar]
  3. Cavarec L., Jensen S., Casella J.-F., Cristescu S. A., Heidmann T. 1997; Molecular cloning and characterization oftranscription factor for the copia retrotransposon with homology to the BTB-containing Lola neurogenic factor. Molecular and Cellular Biology 17:482–494
    [Google Scholar]
  4. Dasgupta P., Saikumar P., Reddy C. D., Reddy P. 1990; Myb protein binds to human immunodeficiency virus 1 long terminal repeat (LTR) sequences and transactivates LTR-mediated transcription. Proceedings of the National Academy of Sciences, USA 87:8090–8094
    [Google Scholar]
  5. Dasgupta P., Reddy C. D., Saikumar P., Reddy P. 1992; The cellular proto-oncogene product Myb acts as transcriptional activator of the long terminal repeat of human T-lymphotropic virus type I. Journal of Virology 66:270–276
    [Google Scholar]
  6. Feuchter A., Mager D. 1990; Functional heterogeneity of a large family of human LTR-like promoters and enhancers. Nucleic Acids Research 18:1261–1270
    [Google Scholar]
  7. Feuchter A. E., Mager D. L. 1992; SV40 large T antigen trans- activates the long terminal repeats of a large family of human endogenous retrovirus-like sequences. Virology 187:242–250
    [Google Scholar]
  8. Feuchter A. E., Freeman J. D., Mager D. L. 1992; Strategy for detecting cellular transcripts promoted by human endogenous long terminal repeats: identification of a novel gene (CDC4L) with homology to yeast CDC4. Genomics 13:1237–1246
    [Google Scholar]
  9. Feuchter-Murthy A. E., Freeman J. D., Mager D. L. 1993; Splicing of a human endogenous retrovirus to a novel phospholipase A2 related gene. Nucleic Acids Research 21:135–143
    [Google Scholar]
  10. Gerondakis S., Bishop J. M. 1986; Structure of the protein encoded by the chicken proto-oncogene c-myb. Molecular and Cellular Biology 6:3677–3684
    [Google Scholar]
  11. Goodchild N. L., Wilkinson D. A., Mager D. L. 1993; Recent evolutionary expansion of a subfamily of RTVL-H human endogenous retrovirus-like elements. Virology 196:778–788
    [Google Scholar]
  12. Hirose Y., Takamatsu M., Harada F. 1993; Presence of env genes in members of the RTLV-H family of human endogenous retrovirus-like elements. Virology 192:52–61
    [Google Scholar]
  13. Kelleher C. A., Wilkinson D. A., Freeman J. D., Mager D. L., Gelfand E. W. 1996; Expression of novel transposon-containing mRNAs in human T cells. Journal of General Virology 77:1101–1110
    [Google Scholar]
  14. Kowalski P. E., Mager D. L. 1998; A human endogenous retrovirus suppresses translation of an associated fusion transcript, PLA2L. Journal of Virology 72:6164–6168
    [Google Scholar]
  15. Kowalski P. E., Freeman J. D., Nelson D. T., Mager D. L. 1997; Genomic structure and evolution ofanovel gene (PLA2L) with duplicated phospholipase A2-like domains. Genomics 39:38–46
    [Google Scholar]
  16. Lindeskog M., Medstrand P., Blomberg J. 1993; Sequence variation of human endogenous retrovirus ERV9-related elements in an env region corresponding to an immunosuppressive peptide: transcription in normal and neoplastic cells. Journal of Virology 67:1122–1126
    [Google Scholar]
  17. Liu A. Y., Abraham B. A. 1991; Subtractive cloning of a hybrid human endogenous retrovirus and calbindin gene in the prostate cell line PC3. Cancer Research 51:4107–4110
    [Google Scholar]
  18. Löwer R., Lower J., Kurth R. 1996; The viruses in all of us: characteristics and biological significance of human endogenous retrovirus sequences. Proceedings of the National Academy of Sciences, USA 93:5177–5184
    [Google Scholar]
  19. Mager D. L. 1989; Polyadenylation function and sequence variability of the long terminal repeats of the human endogenous retrovirus-like family RTVL-H. Virology 173:591–599
    [Google Scholar]
  20. Mager D. L., Freeman J. D. 1987; Human endogenous retroviruslike genome with type C pol sequences and gag sequences related to human T-cell lymphotrophic viruses. Journal of Virology 61:4060–4066
    [Google Scholar]
  21. Mager D. L., Henthorn P. S. 1984; Identification of a retrovirus-like repetitive element in human DNA. Proceedings of the National Academy of Sciences, USA 81:7510–7514
    [Google Scholar]
  22. Medstrand P., Lindeskog M., Blomberg J. 1992; Expression of human endogenous retroviral sequences in peripheral blood mononuclear cells of healthy individuals. Journal of General Virology 73:2463–2466
    [Google Scholar]
  23. Miller W. H. J., Moy D., Li A., Grippo J. F., Dmitrovsky E. 1990; Retinoic acid induces down-regulation of several growth factors and proto-oncogenes in a human embryonal cancer cell line. Oncogene 5:511–517
    [Google Scholar]
  24. Nelson D. T., Goodchild N. L., Mager D. L. 1996; Gain of SP1 sites and loss of repressor sequences associated with a young transcriptionally active subset of HERV-H endogenous LTR. Virology 220:213–218
    [Google Scholar]
  25. Ness S. A., Marknell A., Graf T. 1989; The v-myb oncogene product binds to and activates the promyelocyte-specific mim-1 gene. Cell 59:1115–1125
    [Google Scholar]
  26. Sjottem E., Anderssen S., Johansen T. 1996; The promoter activity of long terminal repeats of the HERV-H family of human retrovirus-like elements is critically dependent on Sp1 family protein interacting with a GC/GT box located immediately 37 to the TATA box. Journal of Virology 70:188–198
    [Google Scholar]
  27. Thompson M. A., Ramsay R. G. 1994; Myb: an old oncoprotein with new roles. BioEssays 17:341–350
    [Google Scholar]
  28. Urnovitz H. B., Murphy W. H. 1996; Human endogenous retroviruses: nature, occurrence, and clinical implications in human disease. Clinical Microbiology Reviews 9:72–99
    [Google Scholar]
  29. Wilkinson D. A., Freeman J. D., Goodchild N. L., Kelleher C. A., Mager D. L. 1990; Autonomous expression of RTVL-H endogenous retrovirus-like elements inhuman cells. JournalofVirology 64:2157–2167
    [Google Scholar]
  30. Wilkinson D. A., Goodchild N. L., Saxton T. M., Wood S., Mager D. L. 1993; Evidence of a functional subclass of the RTLV-H family of human endogenous retrovirus-like sequences. Journal of Virology 67:2981–2989
    [Google Scholar]
  31. Wilkinson D. A., Mager D. L., Leong J.-A. C. 1994; Endogenous human retroviruses. In The Retroviridae pp 465–535 Edited by Levy J. A. New York: Plenum Press;
    [Google Scholar]
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