The role of polyhedrin in the occlusion of virions was studied by substituting two heterologous polyhedrin-coding sequences, one from a multiple-nucleocapsid (M) nucleopolyhedrovirus (NPV) of Spodoptera exigua (Se) and one from a single-nucleocapsid (S) NPV of Buzura suppressaria (BusuNPV), into the genome of Autographa californica (Ac) MNPV. Both heterologous polyhedrin genes were highly expressed and polyhedra were produced in the nuclei of cells infected with the respective recombinant AcMNPVs. Polyhedra produced by the recombinant with BusuNPV polyhedrin showed normal occlusion of multiple-nucleocapsid virions and were equally as infectious to S. exigua larvae as were wild-type AcMNPV polyhedra. This indicates that virion occlusion is not specific with respect to whether the virions or polyhedrin are from an SNPV or MNPV. Polyhedra produced by the recombinant containing the SeMNPV polyhedrin had an altered morphology, being pyramidal rather than polyhedral in shape, and many fewer virions were occluded. These occlusion bodies were less infectious to S. exigua larvae than were those of wild-type AcMNPV. These results indicate that virion occlusion is a finely controlled process that is to some extent specific to the polyhedrin involved and may also require other viral or host factors for optimal morphogenesis.
AyresM. D.,
HowardS. C.,
KuzioJ.,
Lopez-FerberM.,
PosseeR. D.1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605
CaballeroP.,
ZuidemaD.,
Santiago-AlvarezC.,
VlakJ. M.1992; Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Biocontrol Science and Technology 2:145–147
CarstensE. B.,
KrebsA.,
GallerneaultC. E.1986; Identification of an amino acid essential to the normal assembly of Autographa californica nuclear polyhedrosis virus polyhedra. Journal of Virology 58:684–688
CarstensE. B.,
YeL.-B.,
FaulknerP.1987; A point mutation in the polyhedrin gene of a baculovirus, Autographa californica MNPV, prevents crystallization of occlusion bodies. Journal of General Virology 68:901–905
GonzalezM. A.,
SmithG. E.,
SummersM. D.1989; Insertion ofthe SfMNPV polyhedrin gene into an AcMNPV polyhedrin deletion mutant during viral infection. Virology 170:160–175
GrossC. H.,
RussellR. L. Q.,
RohrmannG. F.1994; Orgyia pseudotsugata baculovirus p10 and polyhedron envelope protein genes: analysis of their relative expression levels and role in polyhedron structure. Journal of General Virology 75:1115–1123
HarrisonR. L.,
SummersM. D.1995b; Mutations in the Autographa californica multinucleocapsid nuclear polyhedrosis virus 25 kDa protein gene result in reduced virion occlusion, altered intranuclear envelopment and enhanced virus production. Journal of General Virology 76:1451–1459
Hooft van IddekingeB. J. L.,
SmithG. E.,
SummersM. D.1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
HuZ. H.,
ArifB. M.,
JinF.,
MartensJ. W. M.,
ChenX. W.,
SunJ. S.,
ZuidemaD.,
GoldbachR. W.,
VlakJ. M.1998; Distinct gene arrangement in the Buzura suppressaria single-nucleocapsid nucleo-polyhedrovirus genome. Journal of General Virology 79:2841–2851
JarvisD. L.,
BohlmeyerD. A.,
GarciaA.Jr1991; Requirements for nuclear localization and supramolecular assembly of a baculovirus polyhedrin protein. Virology 185:795–810
JarvisD. L.,
BohlmeyerD. A.,
GarciaA.Jr1992; Enhancement of polyhedrin nuclear localization during baculovirus infection. Journal of Virology 66:6903–6911
MinionF. C.,
CoonsL. B.,
BroomeJ. R.1979; Characterization of the polyhedral envelope of the nuclear polyhedrosis virus of Heliothis virescens
. Journal of Invertebrate Pathology 34:303–307
MurphyF. A.,
FauquetC. M.,
BishopD. H. L.,
GhabrialS. A.,
JarvisA. W.,
MartelliG. P.,
MayoM. A.,
SummersM. D.
(editors) 1995Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses Vienna & New York: Springer-Verlag;
RoosienJ.,
UsmanyM.,
Klinge-RoodeE. C.,
MeijerinkP. H. S.,
VlakJ. M.1986; Heterolog ous recombination between baculoviruses. In Fundamental and Applied Aspects of Invertebrate Pathology pp 389–392 Edited by
SamsonR. A.,
VlakJ. M.,
PetersD.
Wageningen: Foundation of the Fourth International Colloquium of Invertebrate Pathology;
RussellR. M.,
RobertsonJ. L.,
SavinN. E.1977; POLO: a new computer program for probit analysis. Bulletin of the Entomological Society of America 23:209–213
Van LentJ. W. M.,
GroenenJ. T. M.,
Klinge-RoodeE. C.,
RohrmannG. F.,
ZuidemaD.,
VlakJ. M.1990; Localization of the 34 kDa polyhedron envelope protein in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus. Archives of Virology 111:103–114
Van StrienE. A.,
ZuidemaD.,
GoldbachR. W.,
VlakJ. M.1992; Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis virus. Journal of General Virology 73:2813–2821
VaughnJ. L.,
GoodwinR. H.,
TompkinsG. J.,
McCawleyP.1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13:213–217
VlakJ. M.,
RohrmannG. F.1985; The nature of polyhedrin. In Viral Insecticides for Biological Control pp 489–544 Edited by
MaramoroshK.,
ShermanK. E.
Orlando: Academic Press;
WhittM. A.,
ManningJ. S.1988; A phosphorylated 34-kDa protein and a subpopulation of polyhedrin are thiol linked to the carbohydrate layer surrounding a baculovirus occlusion body. Virology 163:33–42
ZhouC. E.,
KoR.,
MaedaS.1998; Polyhedron -like inclusion body formation by a mutant nucleopolyhedrovirus expressing the granulin gene from a granulovirus. Virology 240:282–294
ZuidemaD.,
Klinge-RoodeE. C.,
van LentJ. W. M.,
VlakJ. M.1989; Construction and analysis of an Autographa californica nuclear polyhedrosis virus mutant lacking the polyhedral envelope. Virology 173:98–108
ZuidemaD.,
SchoutenA.,
UsmanyM.,
MauleA. J.,
BelshamG. J.,
RoosienJ.,
Klinge-RoodeE. C.,
van LentJ. W. M.,
VlakJ. M.1990; Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector. Journal of General Virology 71:2201–2209