1887

Abstract

We have studied the evolution of hepatitis C virus (HCV) from a common source following serial transmission from contaminated batches of anti-D immunoglobulin. Six secondary recipients were each infected with virus from identifiable primary recipients of HCV-contaminated anti-D immunoglobulin. Phylogenetic analysis of virus E1/E2 gene sequences [including the hypervariable region (HVR)] and part of NS5B confirmed their common origin, but failed to reproduce the known epidemiological relationships between pairs of viruses, probably because of the frequent occurrence of convergent substitutions at both synonymous and nonsynonymous sites. There was no evidence that the rate at which the HCV genome evolves is affected by transmission events. Three different mechanisms appear to have been involved in generating variation of the hypervariable region; nucleotide substitution, insertion/deletion of nucleotide triplets at the E1/E2 boundary and insertion of a duplicated segment replacing almost the entire HVR. These observations have important implications for the phylogenetic analysis of HCV sequences from epidemiologically linked isolates.

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1999-03-01
2022-01-22
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References

  1. Abe K., Inchauspe G., Fujisawa K. 1992; Genomic characterization and mutation rate of hepatitis C virus isolated from a patient who contracted hepatitis during an epidemic of non-A, non-B hepatitis in Japan. Journal of General Virology 73:2725–2729
    [Google Scholar]
  2. Chamberlain R. W., Adams N. J., Taylor L. A., Simmonds P., Elliott R. M. 1997; The complete coding sequence of hepatitis C virus genotype 5a, the predominant genotype in South Africa. Biochemical and Biophysical Research Communications 236:44–49
    [Google Scholar]
  3. Davidson F., Simmonds P., Ferguson J. C., Jarvis L. M., Dow B. C., Follett E. A. C., Seed C. R. G., Krusius T., Lin C., Medgyesi G. A., Kiyokawa H., Olim G., Duraisamy G., Cuypers T., Saeed A. A., Teo D., Conradie J., Kew M. C., Lin M., Nuchaprayoon C., Ndimbie O. K., Yap P. L. 1995; Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5′ non-coding region. Journal of General Virology 76:1197–1204
    [Google Scholar]
  4. Esteban J. I., Gomez J., Martell M., Cabot B., Quer J., Camps J., Gonzalez A., Otero T., Moya A., Esteban R., Guardia J. 1996; Transmission of hepatitis C virus by a cardiac surgeon. New England Journal of Medicine 334:555–560
    [Google Scholar]
  5. Farci P., Shimoda A., Wong D., Cabezon T., De Gioannis D., Strazzera A., Shimizu Y., Shapiro M., Alter H. J., Purcell R. H. 1996; Prevention of hepatitis C virus infection in chimpanzees by hyperimmune serum against the hypervariable region 1 of the envelope 2 protein. Proceedings of the National Academy of Sciences, USA 93:153941–5399
    [Google Scholar]
  6. Hohne M., Schreier E., Roggendorf M. 1994; Sequence variability in the env-coding region of hepatitis C virus isolated from patients infected during a single source outbreak. Archives of Virology 137:25–34
    [Google Scholar]
  7. Jarvis L. M., Watson H. G., McOmish F., Peutherer J. F., Ludlam C. A., Simmonds P. 1994; Frequent reinfection and reactivation of hepatitis C virus genotypes in multitransfused hemophiliacs. Journal of Infectious Diseases 170:1018–1022
    [Google Scholar]
  8. Kato N., Ootsuyama Y., Tanaka T., Nakagawa M., Nakazawa T., Muraiso K., Ohkoshi S., Hijikata M., Shimotohno K. 1992; Marked sequence diversity in the putative envelope proteins of hepatitis C viruses. Virus Research 22:107–123
    [Google Scholar]
  9. Kojima M., Osuga T., Tsuda F., Tanaka T., Okamoto H. 1994; Influence of antibodies to the hypervariable region of e2/NSl glycoprotein on the selective replication of hepatitis C virus in chimpanzees. Virology 204:665–672
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 1993 MEGA: Molecular Evolutionary Genetics Analysis, version 1.02 Pennsylvania: Pennsylvania State University;
    [Google Scholar]
  11. Lawlor E., Power J., Garson J. A., Yap P. L., Davidson F., Columb G., Smith D. B., Pomeroy L., O’Riordan J., Simmonds P., Tedder R. S. 1999; Transmission rates of HCV by different batches of a contaminated anti-D immunoglobulin preparation. Vox Sanguinis (in press)
    [Google Scholar]
  12. McAllister J., Casino C., Davidson F., Power J., Lawlor E., Yap P. L., Simmonds P., Smith D. B. 1998; Long-term evolution of the hypervariable region of hepatitis C virus in a common-source-infected cohort. Journal of Virology 72:4893–4905
    [Google Scholar]
  13. Ohto H., Terazawa S., Sasaki N., Hino K., Ishiwata C., Kako M., Ujiie N., Endo C., Matsui A., Okamoto H., Mishiro S., Kojima M., Aikawa T., Shimoda K., Sakamoto M., Akahane Y., Yoshizawa H., Tanaka T., Tokita H., Tsuda F. 1994; Transmission of hepatitis C virus from mothers to infants. New England Journal of Medicine 330:744–750
    [Google Scholar]
  14. Power J. P., Lawlor E., Davidson F., Yap P. L., Kenny-Walsh E., Whelton M. J., Walsh T. J. 1994; Hepatitis C viraemia in recipients of Irish intravenous anti-D immunoglobulin. Lancet 344:1166–1167
    [Google Scholar]
  15. Power J. P., Lawlor E., Davidson F., Holmes E. C., Yap P. L., Simmonds P. 1995; Molecular epidemiology of an outbreak of infection with hepatitis C virus in recipients of anti-D immunoglobulin. Lancet 345:1211–1213
    [Google Scholar]
  16. Sekiya H., Kato N., Ootsuyama Y., Nakazawa T., Yamauchi K., Shimotohno K. 1994; Genetic alterations of the putative envelope proteins encoding region of the hepatitis C virus in the progression to relapsed phase from acute hepatitis: humoral immune response to hypervariable region 1. International Journal of Cancer 57:664–670
    [Google Scholar]
  17. Sherman K. E., Andreatta C., O’Brien J., Gutierrez A., Harris R. 1996; Hepatitis C in human immunodeficiency virus-coinfected patients: increased variability in the hypervariable envelope coding domain. Hepatology 23:688–694
    [Google Scholar]
  18. Smith D. B., McAllister J., Casino C., Simmonds P. 1997a; Virus ‘quasispecies’: making a mountain out of a molehill?. Journal of General Virology 78:1511–1519
    [Google Scholar]
  19. Smith D. B., Pathirana S., Davidson F., Lawlor E., Power J., Yap P. L., Simmonds P. 1997b; The origin of hepatitis C virus genotypes. Journal of General Virology 78:321–328
    [Google Scholar]
  20. Taniguchi S., Okamoto H., Sakamoto M., Kojima M., Tsuda F., Tanaka T., Munekata E., Muchmore E. E., Peterson D. A., Mishiro S. 1993; A structurally flexible and antigenically variable N-terminal domain of the hepatitis C virus E2/NS1 protein – implication for an escape from antibody. Virology 195:297–301
    [Google Scholar]
  21. Tautz N., Meyers G., Stark R., Dubovi E. J., Thiel H. J. 1996; Cytopathogenicity of a pestivirus correlates with a 27-nucleotide insertion. Journal of Virology 70:7851–7858
    [Google Scholar]
  22. Weiner A. J., Thaler M. M., Crawford K., Ching K., Kansopon J., Chien D. Y., Hall J. E., Hu F., Houghton M. 1993; A unique, predominant hepatitis C virus variant found in an infant born to a mother with multiple variants. Journal of Virology 67:4365–4368
    [Google Scholar]
  23. Zanetti A. R., Tanzi E., Paccagnini S., Principi N., Pizzocolo G., Caccamo M. L., Damico E., Cambie G., Vecchi L., Bresciani S., Marin M. G., Padula D., Rodella A., Bulgarelli I., Chiodo F., Magliano E., Miotto G., Muggiasca M. L., Pilloton E., Pozzoli R., Pregliasco F., Romano L., Stringhi C., Dagostino F., Paolillo F., Zapparoli B. 1995; Mother-to-infant transmission of hepatitis C virus. Lancet 345:289–291
    [Google Scholar]
  24. Zhang L. Q., Mackenzie P., Cleland A., Holmes E. C., Leigh Brown A. J., Simmonds P. 1993; Selection for specific sequences in the external envelope protein of human immunodeficiency virus type 1 upon primary infection. Journal of Virology 67:3345–3356
    [Google Scholar]
  25. Zibert A., Schreier E., Roggendorf M. 1995; Antibodies in human sera specific to hypervariable region 1 of hepatitis C virus can block viral attachment. Virology 208:653–661
    [Google Scholar]
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