Kaposi’s sarcoma-associated herpesvirus (human herpesvirus-8) encodes a homologue of the Epstein-Barr virus bZip protein EB1 Free

Abstract

Analysis of the recently completed genomic sequence of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus-8) revealed that ORF 50 encodes a protein with homology to the Epstein-Barr virus (EBV) transcription factor R. In this report, we show that ORF K8, contiguous to ORF 50, is interrupted by two introns and that the spliced RNA is translated into a bZip protein that has homology to the EBV transcription factor EB1. The newly characterized K8 protein forms homodimers but does not heterodimerize with other members of the bZip protein family.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-3-557
1999-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/3/0800557a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-3-557&mimeType=html&fmt=ahah

References

  1. Arvanitakis L., Mesri E. A., Nador R. G., Said J. W., Ash A. S., Knowles D. M., Cesarman E. 1996; Establishment and characterisation of a primary effusion (body cavity-based) lymphoma cell line (BC-3) harbouring Kaposi’s sarcoma-associated herpesvirus (KSHV/HHV8) in the absence of Epstein–Barr virus. Blood 88:2648–2654
    [Google Scholar]
  2. Buisson M., Manet E., Biemont M. C., Gruffat H., Durand B., Sergeant A. 1989; The Epstein–Barr virus (EBV) early protein EB2 is a posttranscriptional activator expressed under the control of EBV transcription factors EB1 and R. Journal of Virology 63:5276–5284
    [Google Scholar]
  3. Cesarman E., Moore P. S., Rao P. H., Inghirami G., Knowles D. M., Chang Y. 1995; In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi′s sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86:2708–2714
    [Google Scholar]
  4. Chang Y.-N., Dong D. L.-Y., Hayward G. S., Hayward D. 1990; The Epstein–Barr virus Zta transactivator: a member of the bZIP family with unique DNA-binding specificity and a dimerization domain that lacks the characteristic heptad leucine zipper motif. Journal of Virology 64:3358–3369
    [Google Scholar]
  5. Chang Y., Cesarman E., Pessin M. S., Lee F., Culpepper J., Knowles D. M., Moore P. S. 1994; Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s Sarcoma. Science 266:1865–1870
    [Google Scholar]
  6. Chavrier P., Gruffat H., Chevallier-Greco A., Buisson M., Sergeant A. 1989; The Epstein–Barr virus (EBV) early promoter DR contains a cis-acting element responsive to the Epstein–Barr virus transactivator EB1 and an enhancer with constitutive and inducible activities. Journal of Virology 63:607–614
    [Google Scholar]
  7. Chevallier-Greco A., Manet E., Chavrier P., Mosnier C., Daillie J., Sergeant A. 1986; Both Epstein–Barr virus (EBV) encoded transacting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter. EMBO Journal 5:3243–3249
    [Google Scholar]
  8. Chevallier-Greco A., Gruffat H., Manet E., Calender A., Sergeant A. 1989; The EBV DR enhancer contains two functionally different domains: domain A is constitutive and cell specific, domain B is transactivated by the EBV early protein R. Journal of Virology 63:615–623
    [Google Scholar]
  9. Countryman J., Miller G. 1985; Activation of expression of latent Epstein–Barr herpesvirus after gene transfer with a small cloned subfragment of heterogeneous viral DNA. Proceedings of the National Academy of Sciences, USA 82:4085–4089
    [Google Scholar]
  10. Cox M. A., Leahy J., Hardwick M. 1990; An enhancer within the divergent promoter of Epstein–Barr Virus responds synergically to the R and Z transactivators. Journal of Virology 64:313–321
    [Google Scholar]
  11. Farrell P. J., Rowe D. T., Rooney C. M., Kouzarides T. 1989; Epstein-Barr virus BZLF1 transactivator specifically binds to a consensus AP-1 site and is related to c-Fos. EMBO Journal 8:127–132
    [Google Scholar]
  12. Fixman E. D., Hayward G. S., Hayward S. D. 1992; Trans-acting requirements for replication of Epstein–Barr virus ori-Lyt. Journal of Virology 66:5030–5039
    [Google Scholar]
  13. Flemington E., Speck S. H. 1990a; Autoregulation ofEpstein-Barr virus putative switch gene BZLF1. Journal of Virology 64:1227–1232
    [Google Scholar]
  14. Flemington E., Speck S. H. 1990b; Evidence for coiled-coil dimer formation by an Epstein–Barr virus transactivator that lacks a heptad repeat of leucine residues. Proceedings of the National Academy of Sciences, USA 87:9459–9463
    [Google Scholar]
  15. Gaidano G., Cechova K., Chang Y., Moore P. S., Knowles D. M., Dalla-Favera R. 1996; Establishment of AIDS-related lymphoma cell lines from lymphomatous effusions. Leukemia 10:1237–1240
    [Google Scholar]
  16. Giot J.-F., Mikaelian I., Buisson M., Manet E., Joab I., Nicolas J.-C., Sergeant A. 1991; Transcriptional interference between the EBV transcription factors EB1 and R: both the activation domain and the basic region are required. Nucleic Acids Research 19:1251–1258
    [Google Scholar]
  17. Gruffat H., Sergeant A. 1994; Characterization of the DNA-binding site repertoire for the Epstein–Barr virus transcription factor R. Nucleic Acids Research 22:1172–1178
    [Google Scholar]
  18. Gruffat H., Manet E., Rigolet A., Sergeant A. 1990; The enhancer factor R of Epstein–Barr virus (EBV) is a sequence-specific DNA binding protein. Nucleic Acids Research 18:6835–6843
    [Google Scholar]
  19. Gruffat H., Duran N., Buisson M., Wild F., Buckland R., Sergeant A. 1992; Characterization of an R–binding site mediating the R-induced activation of the Epstein–Barr virus BMLF1 promoter. Journal of Virology 66:46–52
    [Google Scholar]
  20. Holley-Guthrie E. A., Quinlivan E. B., Mar E. B., Kenney S. 1990; The Epstein–Barr virus (EBV) BMRF1 promoter for the early antigen (EA-D) is regulated by the EBV transactivators, BRLF1 and BZLF1, in a cell specific manner. Journal of Virology 64:3753–3759
    [Google Scholar]
  21. Kieff E. 1996; Epstein–Barr virus and its replication. In Fields Virology pp 2343–2395 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  22. Kouzarides T., Packham G., Cook A., Farrell P. J. 1991; The BZLF1 protein of the EBV has a coiled coil dimerisation domain without a heptad leucine repeat but with homology to the C/EBP leucine zipper. Oncogene 6:195–204
    [Google Scholar]
  23. Lieberman P. M., Hardwick J. M., Sample J., Hayward G. S., Hayward S. D. 1990; The Zta transactivator involved in induction of lytic cycle gene expression in Epstein–Barr virus infected lymphocytes binds to both AP-1 and ZRE sites in target promoter and enhancer regions. Journal of Virology 64:1143–1155
    [Google Scholar]
  24. Manet E., Gruffat H., Trescol-Biemont M.-C., Moreno N., Chambard P., Giot J.-F., Sergeant A. 1989; Epstein–Barr virus bicistronic mRNA generated by facultative splicing code for two transcriptional transactivators. EMBO Journal 8:1819–1826
    [Google Scholar]
  25. Miller G., Rabson M., Heston L. 1984; Epstein–Barr Virus with heterogeneous DNA disrupts latency. Journal of Virology 50:174–182
    [Google Scholar]
  26. Miller G., Heston L., Grogan E., Gradoville L., Rigsby M., Sun R., Shedd D., Kushnaryov V. M., Grossberg S., Chang Y. 1997; Selective switch between latency and lytic replication of Kaposi′s sarcoma herpesvirus and Epstein–Barr virus in dually infected body cavity lymphoma cells. Journal of Virology 71:314–324
    [Google Scholar]
  27. Moore P. S., Gao S.-J., Dominguez G., Cesarman E., Lungu O., Knowles D. M., Garber R., Pellett P. E., McGeoch D. J., Chang Y. 1996; Primary characterization of a herpesvirus agent asociated with Kaposi’s sarcoma. Journal of Virology 70:549–558
    [Google Scholar]
  28. Neipel F., Albrecht J.-C., Fleckenstein B. 1997; Cell-homologous genes in the Kaposi′s sarcoma-associated rhadinovirus human herpesvirus 8: determinants of its pathogenicity?. Journal of Virology 71:4187–4192
    [Google Scholar]
  29. Raab M.-S., Albrecht J.-C., Birkmann A., Yaguboglu S., Lang D., Fleckenstein B., Neipel F. 1998; The immunogenic glycoprotein gp35/37of human herpesvirus 8 is encoded by open reading frame K8.1. Journal of Virology 72:6725–6731
    [Google Scholar]
  30. Renne R., Zhong W., Herndier B., McGrath M., Abbey N., Kedes D., Ganem D. 1996; Lytic growth of Kaposi′s sarcoma-associated herpesvirus (human herpesvirus 8) in culture. Nature Medicine 2:342–346
    [Google Scholar]
  31. Rooney C. M., Rowe D. T., Ragot T., Farrell P. J. 1989; The spliced BZLF1 gene of Epstein–Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle. Journal of Virology 63:3109–3116
    [Google Scholar]
  32. Russo J. J., Bohenzky R. A., Chien M.-C., Chen J., Yan M., Maddalena D., Parry J. P., Peruzzi D., Edelman I. S., Chang Y., Moore P. S. 1996; Nucleotide sequence of the Kaposi sarcoma associated herpesvirus (HHV8). Proceedings of the National Academy of Sciences, USA 93:14862–14867
    [Google Scholar]
  33. Schepers A., Pich D., Hammerschmidt W. 1993; A transcription factor with homology to the AP-1 family links RNA transcription and DNA replication in the lytic cycle of Epstein–Barr virus. EMBO Journal 12:3921–9
    [Google Scholar]
  34. Schulz T. F. 1998; Kaposi′s sarcoma-associated herpesvirus (human herpesvirus-8). Journal ofGeneral Virology 79:1573–1591
    [Google Scholar]
  35. Sun R., Lin S.-F., Gradoville L., Yuan Y., Zhu F., Miller G. 1998; A viral gene that activates lytic cycle expression of Kaposi′s sarcoma-associated herpesvirus. Proceedings of the National Academy of Sciences, USA 95:10866–10871
    [Google Scholar]
  36. Waltzer L., Logeat F., Brou C., Israel A., Sergeant A., Manet E. 1994; The human Jk recombination signal sequence binding protein (RBP-Jk) targets the Epstein-Barr virus EBNA2 protein to its DNA responsive elements. EMBO Journal 13:5633–5638
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-3-557
Loading
/content/journal/jgv/10.1099/0022-1317-80-3-557
Loading

Data & Media loading...

Most cited Most Cited RSS feed