1887

Abstract

Six neutralizing monoclonal antibodies (MAbs) were used to isolate MAb neutralization-resistant (MAR) mutants from a recent European strain of swine vesicular disease virus (SVDV), ITL/9/93. Sequencing of MAR mutants identified two epitopes located at positions analogous to sites 2A (VP2) and 3B (VP3) on poliovirus (PV) which have been previously identified on a Japanese strain of SVDV. A third epitope near to the C terminus of VP1, not previously recognized on SVDV, was tentatively identified in a region analogous to site 1 of PV. A fourth epitope, located in the C-terminal region of VP3, has never before been recognized as a site of neutralization on picornaviruses. All four epitopes were predicted to be surface-exposed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-2-277
1999-02-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/2/0800277a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-2-277&mimeType=html&fmt=ahah

References

  1. Blondel B., Crainic R., Fichet O., Dufraisse G., Cardnea A., Diamond D., Girard M., Horaud F. 1986; Mutations conferring resistance to neutralisation with monoclonals in type 1 poliovirus can be located outside or inside the antibody binding site. Journal of Virology 57:81–90
    [Google Scholar]
  2. Brocchi E., Berlinzani A., Gamba D., De Simone F. 1995; Development of two novel MAb based ELISAs for the detection of antibody and the identification of swine isotypes against swine vesicular disease virus. Journal of Virological Methods 52:155–167
    [Google Scholar]
  3. Brocchi E., Zhang G., Knowles N. J., Wilsden G., McCauley J. W., Marquardt O., Ohlinger V. F., De Simone F. 1997; Molecular epidemiology of recent outbreaks of swine vesicular disease: two genetically and antigenically distinct variants in Europe, 1987–94. Epidemiology and Infection 118:51–61
    [Google Scholar]
  4. Brown F., Wild T. F., Rowe L. W., Underwood B. O., Harris T. J. R. 1976; Comparison of swine vesicular disease virus and coxsackie B5 virus by serological and RNA hybridization methods. Journal of General Virology 31:231–237
    [Google Scholar]
  5. Butcher R. N., Obi T. U., McCullough K. C. 1991; Rapid isolation of monoclonal hybridoma cultures by a ′fusion-cloning′ method: the requirement for aminopterin. Biologicals 19:171–175
    [Google Scholar]
  6. De Castro M. P. 1964; Behaviour of the foot-and-mouth disease virus in cell-culture: susceptibility of the IB-RS-2 cell line. Archives of the Institute of Biology, Sao Paulo 31:63–78
    [Google Scholar]
  7. Kanno T., Inoue T., Wang Y., Sarai A., Yamaguchi S. 1995; Identification of the location of antigenic sites of swine vesicular disease virus with neutralization-resistant mutants. Journal of General Virology 76:3099–3106
    [Google Scholar]
  8. Knowles N. J., McCauley J. W. 1997; Coxsackievirus B5 and the relationship to swine vesicular disease virus. Current Topics in Microbiology and Immunology 223:153–167
    [Google Scholar]
  9. Knowles N. J., Sellers R. F. 1994; Swine vesicular disease. In Handbook of Zoonoses section B: Viral 2nd edn pp 437–444 Edited by Beran G. W. Boca Raton: CRC Press;
    [Google Scholar]
  10. Lin F., Mackay D. K. J., Knowles N. J. 1998; The persistence of swine vesicular disease virus infection in pigs. Epidemiology and Infection 121:459–472
    [Google Scholar]
  11. McCahon D., Crowther J. R., Belsham G. J., Kitson J. D. A., Duchesne M., Have P., Meloen R. H., Morgan D. O., De Simone F. 1989; Evidence for at least four antigenic sites on type O foot-and-mouth disease virus involved in neutralization; identification by single and multiple site monoclonal antibody-resistant mutants. Journal of General Virology 70:639–645
    [Google Scholar]
  12. Marquardt O., Ohlinger V. F. 1995; Differential diagnosis and genetic analysis of the antigenically related swine vesicular disease virus and coxsackieviruses. Journal of Virological Methods 53:189–199
    [Google Scholar]
  13. Minor P. D., Evans D. M. A., Ferguson M., Schild G. C., Westrop G., Almond J. W. 1985; Principle and subsidiary antigenic sites of VP1 involved in the neutralization of poliovirus type 3. Journal of Virology 66:1159–1165
    [Google Scholar]
  14. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  15. Mowat G. N., Darbyshire J. H., Huntley J. F. 1972; Differentiation of a vesicular disease of pigs in Hong Kong from foot-and-mouth disease. Veterinary Record 90:618–621
    [Google Scholar]
  16. Muckelbauer J. K., Kremer M., Minor I., Diana G., Dutko F. J., Groarke J., Pevear D. C., Rossmann M. G. 1995; The structure of coxsackievirus B3 at 3.5 Å resolution. Structure 3:653–667
    [Google Scholar]
  17. Nardelli L., Lodetti G., Gualandi G., Goodridge D., Brown F., Cartwright B. 1968; A foot-and-mouth disease syndrome in pigs caused by an enterovirus. Nature 219:1275–1276
    [Google Scholar]
  18. Nijhar S. K. 1997 Antigenic characterisation of swine vesicular disease virus pp 191 PhD thesis University of Hertfordshire, UK;
    [Google Scholar]
  19. Samuel A. R., Knowles N. J., Samuel G. D., Crowther J. R. 1991; Evaluation of a trapping ELISA for the differentiation of foot-and-mouth disease virus strains using monoclonal antibodies. Biologicals 19:299–310
    [Google Scholar]
  20. Sayle R. A., Milner-White E. J. 1995; RasMol: biomolecular graphics for all. Trends in Biochemical Sciences 20:374–376
    [Google Scholar]
  21. Sherry B., Rueckert R. 1985; Evidence for at least two dominant neutralisation antigens on human rhinovirus 14. Journal of Virology 53:137–143
    [Google Scholar]
  22. Tsuda T., Tokui T., Onodera T. 1987; Induction of neutralising antibodies by structural proteins VP1 and VP2 of swine vesicular disease virus. Japanese Journal of Veterinary Science 49:129–132
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-2-277
Loading
/content/journal/jgv/10.1099/0022-1317-80-2-277
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error