1887

Abstract

The methanogenic archaeobacterium (strain PS) is known to produce a filterable, DNase-resistant agent (called VTA, for transfer agent), which carries very small fragments (4400 bp) of bacterial DNA and is able to transduce bacterial genes between derivatives of the strain. Examination by electron microscopy of two preparations of VTA that were concentrated and partially purified by different methods showed virus-like particles with isometric heads, about 40 nm in diameter, and with 61 nm long tails. These particles co-sedimented with the minute bacteriophage ϕX174 in a sucrose density gradient.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-12-3305
1999-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/12/0803305.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-12-3305&mimeType=html&fmt=ahah

References

  1. Bertani G. 1999; Transduction-like gene transfer in the methanogen Methanococcus voltae. Journal of Bacteriology 181:2992–3002
    [Google Scholar]
  2. Bertani G., Baresi L. 1987; Genetic transformation in the methanogen Methanococcus voltae PS. Journal of Bacteriology 169:2730–2738
    [Google Scholar]
  3. Bertani L. E., Six E. W. 1988; The P2-like phages and their parasite, P4. In The Bacteriophages vol 273–143 Edited by Calendar R. New York & London: Plenum Press;
    [Google Scholar]
  4. Fox G. E., Magrum L. J., Balch W. E., Wolfe R. S., Woese C. R. 1977; Classification of methanogenic bacteria by 16S ribosomal RNA characterization. P roceedings of the National Academy of Sciences. USA 74:4537–4541
    [Google Scholar]
  5. Hayashi M., Aoyama A., Richardson D. L. Jr, Hayashi M. N. 1988; Biology of the bacteriophage ϕX174. In The Bacteriophages pp. 1–71 Edited by Calendar R. New York & London: Plenum Press;
    [Google Scholar]
  6. Jarrell K. F., Koval S. F. 1989; Ultrastructure and biochemistry of Methanococcus voltae. Critical Reviews in Microbiology 17:53–87
    [Google Scholar]
  7. Kalmokoff M. L., Jarrell K. F., Koval S. F. 1988; Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114. Journal of Bacteriology 170:1752–1758
    [Google Scholar]
  8. Karam J. editor 1995 Molecular Biology of Bacteriophage T4 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Meile L., Abenschein P., Leisinger T. 1990; Transduction in the archaebacterium Methanobacterium thermoautotrophicum Marburg. Journal of Bacteriology 172:3507–3508
    [Google Scholar]
  10. Michel M. R., Hirt B., Weil R. 1967; Mouse cellular DNA enclosed in polyoma viral capsids (pseudovirions). Proceedings of the National Academy of Sciences, USA 58:1381–1388
    [Google Scholar]
  11. Olsen G. J., Woese C. R. 1997; Archaeal genomics: an overview. Cell 89:991–994
    [Google Scholar]
  12. Valentine R. C., Shapiro B. M., Stadtman E. R. 1968; Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli. Biochemistry 7:2143–2152
    [Google Scholar]
  13. Wood A. G., Whitman W. B., Konisky J. 1989; Isolation and characterization of an archaebacteral viruslike particle from Methanococcus voltae A3. Journal of Bacteriology 171:93–98
    [Google Scholar]
  14. Yen H. C., Hu N. T., Marrs B. L. 1979; Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata. Journal of Molecular Biology 131:157–168
    [Google Scholar]
  15. Zillig W., Reiter W.-D., Palm P., Gropp F., Neumann H., Rettenberger M. 1988; Viruses of archaebacteria. In The Bacteriophages pp. 517–558 Edited by Calendar R. New York & London: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-12-3305
Loading
/content/journal/jgv/10.1099/0022-1317-80-12-3305
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error