1887

Abstract

The P4 promoter of the parvovirus minute virus of mice (MVMp) directs transcription of the genes encoding non-structural proteins. We have previously shown that functional upstream CRE elements contribute to both the oncogene-dependent activation of promoter P4 and its down-modulation by known activators of cyclic AMP-dependent protein kinase A (PKA). In the present work, the nucleoprotein complexes formed with the P4 CRE elements were characterized with regard to their polypeptide constituents and the nucleotides taking part in the interaction. Atypical interactions, both at the protein–protein and protein–DNA level, were observed, which may be a reflection of the divergence of the parvoviral CREs from the usual consensus. The CRE-mediated regulation of promoter P4 by PKA and Ras is discussed in light of these findings.

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1999-12-01
2019-10-15
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References

  1. Ahn, J. K., Gavin, B. J., Kumar, G. & Ward, D. C. ( 1989; ). Transcriptional analysis of minute virus of mice P4 promoter mutants. Journal of Virology 63, 5425-5239.
    [Google Scholar]
  2. Christensen, J., Cotmore, S. F. & Tattersall, P. ( 1997a; ). A novel cellular site-specific DNA-binding protein cooperates with the viral NS1 polypeptide to initiate parvovirus DNA replication. Journal of Virology 71, 1405-1416.
    [Google Scholar]
  3. Christensen, J., Cotmore, S. F. & Tattersall, P. ( 1997b; ). Parvovirus initiation factor PIF: a novel human DNA-binding factor which coordinately recognizes two ACGT motifs. Journal of Virology 71, 5733-5741.
    [Google Scholar]
  4. Deleu, L., Fuks, F., Spitkovsky, D., Horlein, R., Faisst, S. & Rommelaere, J. ( 1998; ). Opposite transcriptional effects of cyclic AMP-responsive elements in confluent or p27KIP-overexpressing cells versus serum-starved or growing cells. Molecular and Cellular Biology 18, 409-419.
    [Google Scholar]
  5. Ellis, J. C., Lindon, A. C., Flint, K. J., Jones, N. C. & Goodburn, S. ( 1995; ). Activating transcription factor-1 is a specific antagonist of the cyclic adenosine 3′,5′-monophosphate (cAMP) response element-binding protein-1-mediated response to cAMP. Molecular Endocrinology 9, 255-265.
    [Google Scholar]
  6. Englander, E. W., Widen, S. G. & Wilson, S. H. ( 1991; ). Mammalian β-polymerase promoter: phosphorylation of ATF/CRE-binding protein regulation of DNA binding. Nucleic Acids Research 19, 3369-3374.[CrossRef]
    [Google Scholar]
  7. Faisst, S., Perros, M., Deleu, L., Spruyt, N. & Rommelaere, J. ( 1994; ). Mapping of upstream regulatory elements in the P4 promoter of parvovirus minute virus of mice. Virology 202, 466-470.[CrossRef]
    [Google Scholar]
  8. Fuks, F., Deleu, L., Dinsart, C., Rommelaere, J. & Faisst, S. ( 1996; ). Ras oncogene-dependent activation of the P4 promoter of minute virus of mice through a proximal P4 element interacting with the Ets family of transcription factors. Journal of Virology 70, 1331-1339.
    [Google Scholar]
  9. Gallo, A., Benusiglio, E., Bonapace, I. M., Feliciello, A., Cassano, S., Garbi, C., Musti, A. M., Gottesman, M. E. & Avvedimento, E. V. ( 1992; ). v-ras and protein kinase C dedifferentiate thyroid cells by down-regulating nuclear cAMP-dependent protein kinase A. Genes & Development 6, 1621-1630.[CrossRef]
    [Google Scholar]
  10. Gonzalez, G. A., Yamamoto, K. K., Fischer, W. H., Karr, D., Menzel, P., Biggs, W. D., Vale, W. W. & Montminy, M. R. ( 1989; ). A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence. Nature 337, 749-752.[CrossRef]
    [Google Scholar]
  11. Gu, Z., Plaza, S., Perros, M., Cziepluch, C., Rommelaere, J. & Cornelis, J. J. ( 1995; ). NF-Y controls transcription of the minute virus of mice P4 promoter through interaction with an unusual binding site. Journal of Virology 69, 239-246.
    [Google Scholar]
  12. Hai, T., Liu, F., Coukos, W. J. & Green, M. R. ( 1989; ). Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. Genes & Development 3, 2083-2090.[CrossRef]
    [Google Scholar]
  13. Hurst, H. C. ( 1995; ). Transcription factors 1. bZIP proteins. In Protein Profiles, pp. 105-168. Edited by P. Sheterline. London: Academic Press.
  14. Hurst, H. C., Masson, N., Jones, N. C. & Lee, K. A. ( 1990; ). The cellular transcription factor CREB corresponds to activating transcription factor 47 (ATF-47) and forms complexes with a group of polypeptides related to ATF-43. Molecular and Cellular Biology 10, 6192-6203.
    [Google Scholar]
  15. Kuwabara, M. D. & Sigman, D. S. ( 1987; ). Footprinting DNA–protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase–lac promoter complexes. Biochemistry 26, 7234-7238.[CrossRef]
    [Google Scholar]
  16. Montminy, M. R. ( 1997; ). Transcriptional regulation by cyclic AMP. Annual Review in Biochemistry 66, 807-822.[CrossRef]
    [Google Scholar]
  17. Montminy, M. R. & Bilezikjian, L. M. ( 1987; ). Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328, 175-178.[CrossRef]
    [Google Scholar]
  18. Perros, M., Deleu, L., Vanacker, J. M., Kherrouche, Z., Spruyt, N., Faisst, S. & Rommelaere, J. ( 1995; ). Upstream CREs participate in the basal activity of minute virus of mice promoter P4 and in its stimulation in ras-transformed cells. Journal of Virology 69, 5506-5515.
    [Google Scholar]
  19. Rehfuss, R. P., Walton, K. M., Loriaux, M. M. & Goodman, R. H. ( 1991; ). The cAMP-regulated enhancer-binding protein ATF-1 activates transcription in response to cAMP-dependent protein kinase A. Journal of Biological Chemistry 266, 18431-18434.
    [Google Scholar]
  20. Rommelaere, J. & Cornelis, J. J. ( 1991; ). Antineoplastic activity of parvoviruses. Journal of Virological Methods 33, 233-251.[CrossRef]
    [Google Scholar]
  21. Rommelaere, J. & Tattersall, P. ( 1990; ). Oncosuppression by parvoviruses. In Handbook of Parvoviruses, pp. 41-57. Edited by P. Tijsen. Boca Raton, FL: CRC Press.
  22. Ruppert, S., Cole, T. J., Boshart, M., Schmid, E. & Schutz, G. ( 1992; ). Multiple mRNA isoforms of the transcription activator protein CREB: generation by alternative splicing and specific expression in primary spermatocytes. EMBO Journal 11, 1503-1512.
    [Google Scholar]
  23. Spegelaere, P., van Hille, B., Spruyt, N., Faisst, S., Cornelis, J. J. & Rommelaere, J. ( 1991; ). Initiation of transcription from the minute virus of mice P4 promoter is stimulated in rat cells expressing a c-Ha-ras oncogene. Journal of Virology 65, 4919-4928.
    [Google Scholar]
  24. Sturm, R., Baumruker, T., Franza, B. R. J. & Herr, W. ( 1987; ). A 100 kD HeLa cell octamer binding protein (OBP100) interacts differently with two separate octamer-related sequences within the SV40 enhancer. Genes & Development 1, 1147-1160.[CrossRef]
    [Google Scholar]
  25. Vanacker, J.-M. & Rommelaere, J. ( 1995; ). Non-structural proteins of autonomous parvoviruses: from cellular effects to molecular mechanisms. Seminars in Virology 6, 291-297.[CrossRef]
    [Google Scholar]
  26. von der Ahe, D., Pearson, D. & Nagamine, Y. ( 1990; ). Macromolecular interaction on a cAMP responsive region in the urokinase-type plasminogen activator gene: a role of protein phosphorylation. Nucleic Acids Research 18, 1991-1999.[CrossRef]
    [Google Scholar]
  27. Weih, F., Stewart, A. F., Boshart, M., Nitsch, D. & Schutz, G. ( 1990; ). In vivo monitoring of a cAMP-stimulated DNA-binding activity. Genes & Development 4, 1437-1449.[CrossRef]
    [Google Scholar]
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