1887

Abstract

Bovine viral diarrhoea virus (BVDV) is an economically important pathogen of cattle that is ubiquitously distributed worldwide. In this study, cattle were immunized by intramuscular injections with plasmid DNA expressing the BVDV type 1 major glycoprotein E2. Animals either received injections of naked DNA (N-DNA) or DNA in cationic liposomes (L-DNA). Both DNA preparations induced virus-specific neutralizing antibodies in vaccinates, although the response was much lower in N-DNA-immunized animals. N-DNA-vaccinated animals also showed virus-specific lymphocyte proliferation responses to type 1, live BVDV , whereas L-DNA vaccination induced no such responses. After 16 weeks, DNA-vaccinated and mock-vaccinated animals were challenged with a USDA-certified BVDV type 1 strain. Four significant observations were made: (1) N-DNA-vaccinated calves showed limited protection from virus challenge, (2) L-DNA-vaccinated animals did not show any signs of protection, (3) the challenge induced strong memory responses in the production of serum neutralizing antibodies to both genotypes (type 1 and 2 of BVDV), and (4) the challenge induced a mucosal memory response in nasal secretions of both L- and N-DNA-vaccinated animals.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-12-3137
1999-12-01
2020-07-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/12/0803137.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-12-3137&mimeType=html&fmt=ahah

References

  1. Bangham A. D., Standish M. M., Watkins J. C.. 1965; Diffusion of univalent ions across the lamellae of swollen phospholipids. Journal of Molecular Biology13:238–252
    [Google Scholar]
  2. Bolin S. R.. 1990; The current understanding about the pathogenesis and clinical forms of BVD. Veterinary Medicine85:1124–1132
    [Google Scholar]
  3. Bolin S. R.. 1995; Control of bovine viral diarrhea infection by use of vaccination. Veterinary Clinics of North America Food Animal Practice11:615–625
    [Google Scholar]
  4. Bolin S. R., Ridpath J. F.. 1996; Glycoprotein E2 of bovine viral diarrhea virus expressed in insect cells provides calves with limited protection from systemic infection and disease. Archives of Virology141:1463–1477
    [Google Scholar]
  5. Bruschke C. J., Moormann R. J., van Oirschot J. T., van Rijn P. A.. 1997; A subunit based on glycoprotein E2 of bovine virus diarrhea induces fetal protection in sheep against homologous challenge. Vaccine15:1940–1945
    [Google Scholar]
  6. Collett M. S., Larson R., Belzer S. K., Retzel E.. 1988; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology165:200–208
    [Google Scholar]
  7. Cortese V. S., West K. H., Hassard L. E., Carman S., Ellis J. A.. 1998; Clinical and immunological responses of vaccinated and unvaccinated calves to infection with a virulent type-II isolate of bovine viral diarrhea virus. Journal of the American Veterinary Medical Association1:1312–1319
    [Google Scholar]
  8. Cox G. J. M., Zamb T. J., Babiuk L. A.. 1993; Bovine herpesvirus 1: immune responses in mice and cattle injected with plasmid DNA. Journal of Virology67:5664–5667
    [Google Scholar]
  9. Donis R. O.. 1995; Molecular biology of bovine viral diarrhea virus and its interactions with the host. Veterinary Clinics of North America Food Animal Practice11:393–423
    [Google Scholar]
  10. Donis R. O., Corapi W., Dubovi E. J.. 1988; Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56K to 58K glycoprotein. Journal of General Virology69:77–86
    [Google Scholar]
  11. Donnelly J. J., Ulmer J. B., Shiver J. W., Liu M. A.. 1997; DNA vaccines. Annual Review of Immunology15:617–648
    [Google Scholar]
  12. Elahi S. H., Shen S.-H., Harpin S., Talbot B. G., Elazhary Y.. 1999; Investigation of the immunological properties of the bovine viral diarrhea virus protein NS3 expressed by an adenovirus vector in mice. Archives of Virology144:1057–1070
    [Google Scholar]
  13. Felgner P. L., Gadek T. R., Holm M., Roman R., Chan H. W., Wenz M., Northrop J. P., Ringold G. M., Danielsen M.. 1987; Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proceedings of the National Academy of Sciences, USA84:7413–7417
    [Google Scholar]
  14. Gerdts V., Jöns A., Makoschey B., Visser N., Mettenleiter T. C.. 1997; Protection of pigs against Aujeszky’s disease by DNA vaccination. Journal of General Virology78:2139–2146
    [Google Scholar]
  15. Harpin S., Elahi S. M., Cornaglia E., Yolken R. H., Elazhary Y.. 1995; The 5′-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus. Archives of Virology140:1285–1290
    [Google Scholar]
  16. Harpin S., Talbot B., Mbikay M., Elazhary Y.. 1997; Immune response to vaccination with DNA encoding the bovine viral diarrhea virus major glycoprotein gp53 (E2. FEMS Microbiology Letters146:229–234
    [Google Scholar]
  17. Hasset D., Whitton J. L.. 1996; DNA immunization. Trends in Microbiology4:307–312
    [Google Scholar]
  18. Horzinek M. C.. 1991; Pestiviruses – taxonomic perspectives. Archives of Virology Supplementum3:55–65
    [Google Scholar]
  19. Liess B., Orban S., Frey H. R., Trautwien G., Wiefel W., Blindow H.. 1984; Studies on transplacental transmissibility of a bovine virus diarrhea (BVD) vaccine virus in cattle. Zentralblatt fur Veterinarmedizin Reihe B31:669–681
    [Google Scholar]
  20. Pellerin C., Van Den Hurk J., Lecomte J., Tijssen P.. 1994; Identification of a new group of bovine viral diarrhea virus strains associated with severe outbreak and high mortalities. Virology203:260–268
    [Google Scholar]
  21. Pirzadeh B., Dea S.. 1998; Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. Journal of General Virology79:989–999
    [Google Scholar]
  22. Ridpath J. F., Bolin S. R., Dubovi E. J.. 1994; Segregation of bovine viral diarrhea virus into genotypes. Virology205:66–74
    [Google Scholar]
  23. Robinson H. L., Hunt L. A., Webster R. G.. 1993; Protection against lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA. accine11:957–960
    [Google Scholar]
  24. Roth J. A., Kaeberle M. L.. 1983; Suppression of neutrophil and lymphocyte function induced by a vaccinal strain of bovine viral diarrhea virus with or without administration of ACTH. American Journal of Veterinary Research44:2366–2372
    [Google Scholar]
  25. Schultz R. D.. 1993; Certain factors to consider when designing a bovine vaccination program. In Proceedings of the 26th Annual Convention of the American Association of Bovine Practitioners pp.19–26 Edited by Williams E. I.. Stillwater, OK, USA: Frontier Printers;
    [Google Scholar]
  26. Sedegah M., Hedstrom R., Hobart P., Hoffman S. L.. 1994; Protection against malaria by immunization with plasmid DNA encoding circumsporozoite protein. Proceedings of the National Academy of Sciences, USA9:9866–9870
    [Google Scholar]
  27. Siegrist C. A., Lambert P. H.. 1997; Immunization with DNA vaccines in early life: advantages and limitations as compared to conventional vaccines. Springer Seminars in Immunopathology19:233–243
    [Google Scholar]
  28. van Drunen Littel-van den Hurk S., Braun R. P., Lewis P. J., Karvonen B. C., Baca-Estrada M. E., Snider M., McCartney D., Watts T., Babiuk L. A.. 1998; Intradermal immunization with a bovine herpesvirus-1 DNA vaccine induces protective immunity in cattle. Journal of General Virology79:831–839
    [Google Scholar]
  29. Xiang Z. Q., Spitalnik S., Tran M., Wunner W. H., Cheng J., Ertl H. C. J.. 1994; Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology199:132–140
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-12-3137
Loading
/content/journal/jgv/10.1099/0022-1317-80-12-3137
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error