1887

Abstract

Recombinant lentogenic Newcastle disease virus (NDV) of the vaccine strain Clone-30 was reproducibly generated after simultaneous expression of antigenome-sense NDV RNA and NDV nucleoprotein, phosphoprotein and RNA-dependent RNA polymerase from plasmids transfected into cells stably expressing T7 RNA polymerase. For this purpose, the genome of Clone-30, comprising 15186 nt, was cloned and sequenced prior to assembly into a full-length cDNA clone under control of a T7 RNA polymerase promoter. Recombinant virus was amplified by inoculation of transfection supernatant into the allantoic cavity of embryonated specific-pathogen-free (SPF) chicken eggs. Two marker restriction sites comprising a total of five nucleotide changes artificially introduced into noncoding regions were present in the progeny virus. The recombinant NDV was indistinguishable from the parental wild-type virus with respect to its growth characteristics in cell culture and in embryonated eggs. Moreover, an intracerebral pathogenicity index of 0·29 was obtained for both viruses as determined by intracerebral inoculation of day-old SPF chickens, proving that the recombinant NDV is a faithful copy of the parental vaccine strain of NDV.

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1999-11-01
2020-05-27
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References

  1. Alexander D. J.. American Association of Avian Pathologists 1989; Newcastle disease.. In A Laboratory Manual for the Isolation and Identification of Avian Pathogens pp114–120 Edited by Iowa: Kendall/Hunt Publishing Company;
    [Google Scholar]
  2. Alexander D. J.. 1997; Newcastle disease and other avian Paramyxoviridae infections. In Diseases of Poultry pp541–569 Edited by Calnek B. W.. Ames, Iowa: Mosby–Wolfe Iowa State University Press;
    [Google Scholar]
  3. Baron M. D., Barrett T.. 1997; Rescue of rinderpest virus from cloned cDNA. Journal of Virology71:1265–1271
    [Google Scholar]
  4. Buchholz U., Finke S., Conzelmann K.-K.. 1999; Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. Journal of Virology73:251–259
    [Google Scholar]
  5. Calain P., Roux L.. 1993; The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. Journal of Virology67:4822–4830
    [Google Scholar]
  6. CEC 1992; Council Directive 92/66/EEC of 14 July 1992 introducing Community measures for the control of Newcastle disease. Official Journal of the European CommunitiesL260:1–20
    [Google Scholar]
  7. Collins P. L., Hill M. G., Camargo E., Grosfeld H., Chanock R. M., Murphy B. R.. 1995; Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5′ proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proceedings of the National Academy of Sciences USA92:11563–11567
    [Google Scholar]
  8. Conzelmann K.-K.. 1998; Nonsegmented negative-strand RNA viruses: genetics and manipulation of viral genomes. Annual Review of Genetics32:123–162
    [Google Scholar]
  9. de Leeuw O., Peeters B.. 1999; Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae. Journal of General Virology80:131–136
    [Google Scholar]
  10. Durbin A. P., Hall S. L., Siew J. W., Whitehead S. S., Collins P. L., Murphy B. R.. 1997; Recovery of infectious human parainfluenza virus type 3 from cDNA. Virology235:323–332
    [Google Scholar]
  11. Galinski M. S., Mink M. A., Pons M. W.. 1988; Molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the L protein. Virology165:499–510
    [Google Scholar]
  12. Garcin D., Pelet T., Calain P., Roux L., Curran J., Kolakofsky D.. 1995; A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA: generation of a novel copy-back nondefective interfering virus. EMBO Journal14:6087–6094
    [Google Scholar]
  13. Giesecke H., Obermaier B., Domdey H., Neubert W. J.. 1992; Rapid sequencing of the Sendai virus 6·8 kb large (L) gene through primer walking with an automated DNA sequencer. Journal of Virological Methods38:47–60
    [Google Scholar]
  14. Hanson R. P., Brandly C. A.. 1955; Identification of vaccine strain of Newcastle disease virus. Science122:156–157
    [Google Scholar]
  15. He B., Paterson R. G., Ward C. D., Lamb R. A.. 1997; Recovery of infectious SV5 from cloned DNA and expression of a foreign gene. Virology237:249–260
    [Google Scholar]
  16. Hoffmann M. A., Banerjee A. K.. 1997; An infectious clone of human parainfluenza virus type 3. Journal of Virology71:4272–4277
    [Google Scholar]
  17. Kato A., Kiyotani K., Sakai Y., Yoshida T., Nagai Y.. 1997; The paramyxovirus, Sendai virus, V protein encodes a luxury function required for viral pathogenesis. EMBO Journal16:578–598
    [Google Scholar]
  18. Kolakofsky D., Pelet T., Garcin D., Hausmann S., Curran J., Roux L.. 1998; Paramyxovirus RNA synthesis and the requirement for hexamer genome length: the rule of six revisited. Journal of Virology72:891–899
    [Google Scholar]
  19. Komase K., Rima B. K., Pardowitz I., Kunz C., Billeter M. A., ter Meulen V., Baczko K.. 1995; A comparison of nucleotide sequences of measles virus L genes derived from wild-type viruses and SSPE brain tissues. Virology208:795–799
    [Google Scholar]
  20. Krishnamurthy S., Samal S. K.. 1998; Nucleotide sequences of the trailer, nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence. Journal of General Virology79:2419–2424
    [Google Scholar]
  21. Lawson N. D., Stillmann E. A., Whitt M. A., Rose J. K.. 1995; Recombinant vesicular stomatitis viruses from cDNA. Proceedings of the National Academy of Sciences USA92:4477–4481
    [Google Scholar]
  22. Mundt E., Müller H.. 1995; Complete nucleotide sequences of 5′- and 3′-noncoding regions of both segments of different strains of infectious bursal disease virus. Virology209:10–18
    [Google Scholar]
  23. Oberdörfer A., Werner O.. 1998; Newcastle disease virus: detection and characterization by PCR of recent German isolates differing in pathogenicity. Avian Pathology27:237–243
    [Google Scholar]
  24. Okazaki K., Tanabayashi K., Takeuchi K., Hishiyama M., Okazaki K., Yamada A.. 1992; Molecular cloning and sequence analysis of the mumps virus gene encoding the L protein and the trailer sequence. Virology188:926–930
    [Google Scholar]
  25. Palese P., Zheng H., Engelhardt O. G., Pleschak S., Garcia-Sastre A.. 1996; Negative-strand RNA viruses: genetic engineering and applications. Proceedings of the National Academy of Sciences USA93:11354–11358
    [Google Scholar]
  26. Pattnaik A. K., Ball L. A., LeGrone A. W., Wertz G. W.. 1992; Infectious defective interfering particles of VSV from transcripts of a cDNA clone. Cell69:1011–1020
    [Google Scholar]
  27. Peeters B. P., de Leeuw O. S., Koch G., Gielkens A. L.. 1999; Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. Journal of Virology73:5001–5009
    [Google Scholar]
  28. Phillips R. J., Samson A. C. R., Emmerson P. T.. 1998; Nucleotide sequence of the 5′-terminus of Newcastle disease virus and assembly of the complete genomic sequence: agreement with the ‘rule of six’. Archives of Virology143:1993–2002
    [Google Scholar]
  29. Poch O., Blumberg B. M., Bougueleret L., Tordo N.. 1990; Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. Journal of General Virology71:1153–1162
    [Google Scholar]
  30. Radecke F., Spielhofer P., Schneider H., Kaelin K., Huber M., Dötsch C., Christiansen G., Billeter M. A.. 1995; Rescue of measles virus from cloned DNA. EMBO Journal14:5773–5784
    [Google Scholar]
  31. Rima B. K., Alexander D. J., Billeter M. A., Collins P. L., Kingsbury D. W., Lipkind M. A., Nagai Y., Örvell C., Pringle C. R., ter Meulen V.. 1995; The Paramyxoviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp268–274 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D.. Vienna & New York: Springer-Verlag;
    [Google Scholar]
  32. Rott R., Klenk H.-D.. 1988; Molecular basis of infectivity and pathogenicity of Newcastle disease virus. In Newcastle Disease pp98–112 Edited by Alexander D. J. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: A Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  34. Schnell M. J., Mebatsion T., Conzelmann K.-K.. 1994; Infectious rabies viruses from cloned cDNA. EMBO Journal13:4195–4203
    [Google Scholar]
  35. Steward M., Vipond I. B., Millar N. S., Emmerson P. T.. 1993; RNA editing in Newcastle disease virus. Journal of General Virology74:2539–2547
    [Google Scholar]
  36. Werner O., Römer-Oberdörfer A., Köllner B., Manvell R., Alexander D. J.. 1999; Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996. Avian Pathology28:79–88
    [Google Scholar]
  37. Whelan S. P., Ball L. A., Barr J. N., Wertz G. T. W.. 1995; Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones. Proceedings of the National Academy of Sciences USA92:8388–8392
    [Google Scholar]
  38. Yusoff K., Millar N. S., Chambers P., Emmerson P. T.. 1987; Nucleotide sequence analysis of the L gene of Newcastle disease virus: homologies with Sendai and vesicular stomatitis viruses. Nucleic Acids Research15:3961–3976
    [Google Scholar]
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