1887

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Volume 80, Issue 11

Porcine adenovirus-3 as a helper-dependent expression vector Free

Abstract

Porcine adenovirus has been proposed as a potential vector for generating novel and effective vaccines for pigs. As a prerequisite for the generation of helper-dependent porcine adenovirus-3 (PAV-3) vectors, two E1-complementing porcine cell lines expressing E1 proteins of human adenovirus-5 (HAV-5) were made. These cell lines could be efficiently transfected with DNA and allowed the rescue and propagation of a PAV-3 recombinant, PAV201, containing a 0·597 kb E3 deletion and a 0·803 kb E1A deletion. Our data demonstrate that E1A proteins of HAV-5 have the capacity to transform foetal porcine retina cells and complement for the E1A proteins of PAV-3. The green fluorescent protein (GFP) gene placed under the control of a cytomegalovirus immediate early promoter was inserted into the E1A region of the PAV201 genome. Using these cell lines, a helper-dependent PAV-3 recombinant expressing GFP, PAV202, was constructed and characterized. The wild-type PAV-3 and the recombinant PAV202 expressing GFP were used to determine the ability of the virus to enter and replicate in cells of human and animal origin under cell culture conditions. Our results suggest that PAV-3 enters but does not replicate in dog, sheep, bovine and human cells.

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© Microbiology Society
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1999-11-01
2024-04-19
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References

  1. Bagchi S., Raychaudhuri P., Nevins J. R. 1990; Adenovirus E1A proteins can dissociate heteromeric complexes involving the e2F transcription factor. A novel mechanism for E1A transactivation. Cell 62:659–669
     [Google Scholar]
  2. Ball A. O., Williams M. E., Spindler K. R. 1988; Identification of mouse adenovirus type 1 early region 1: DNA sequence and a conserved transactivating function. Journal of Virology 62:3947–3957
     [Google Scholar]
  3. Clarke M. C., Sharpe H. B. A., Derbyshire J. B. 1967; Some characteristics of three porcine adenoviruses. Archiv für die Gesamte Virusforschung 21:91–97
     [Google Scholar]
  4. Cubitt A. B., Heim T., Adams S. R., Boyd A. E., Gross L. A., Tsien T. Y. 1995; Understanding, improving and using green fluorescent proteins. Trends in Biochemistry 20:448–455
     [Google Scholar]
  5. Debbas M., White E. 1993; Wild-type p53 mediates apoptosis by E1A which is inhibited by E1B. Genes & Development 7:546–554
     [Google Scholar]
  6. Derbyshire J. B., Clarke M. C., Collins A. P. 1975; Serological and pathogenicity studies with some unclassified porcine adenoviruses. Journal of Comparative Pathology 85:437–443
     [Google Scholar]
  7. Fallaux F. J., Kraneburg O., Cramer S. J., Houweling A., Van Ormondt H., Hoeben R. C., Van Der Eb A. J. 1996; Characterization of 911: a new helper cell line for the titration and propagation of early region 1-deleted adenoviral vectors. Human Gene Therapy 7:215–222
     [Google Scholar]
  8. Fallaux F. J., Bout A., Van Der Velde I., Van Den Wollenberg D. J. M., Hehir K. M., Keegan J., Auger C., Cramer S. J., Van Ormondt H., Van Der Eb A. J., Valerio D., Hoeben R. C. 1998; New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. Human Gene Therapy 9:1909–1917
     [Google Scholar]
  9. Fisher P. B., Babiss L. E., Weinstein I. B., Ginsberg H. S. 1982; Analysis of type 5 adenovirus transformation with a cloned rat embryo cell line (CREF. Proceedings of the National Academy of Sciences USA 79:3527–3531
     [Google Scholar]
  10. Gerard R. D., Meidell R. S. 1993; Adenovirus-mediated gene transfer. Trends in Cardiovascular Medicine 3:171–177
     [Google Scholar]
  11. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
     [Google Scholar]
  12. Hehir K. M., Armentano D., Cardoza L. M., Choquette T. L., Berthelette P. B., White G. A., Couture L. A., Everton M. B., Keegan J., Martin J. M., Pratt D. A., Smith M. P., Smith A. E., Wadsworth S. C. 1996; Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence. Journal of Virology 70:8459–8467
     [Google Scholar]
  13. Hirahara T., Yauhara H., Matsui O., Yamanaka M., Tanaka M., Fukuyama S. 1990; Isolation of porcine adenovirus from the respiratory tract of pigs in Japan. Japanese Journal of Veterinary Science 52:407–409
     [Google Scholar]
  14. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
     [Google Scholar]
  15. Imler J. L., Bout A., Dreyer D., Dieterle A., Schultz H., Valerio D., Mehtali M., Pavirani A. 1995; Trans-complementation of E1-deleted adenovirus: a new vector to reduce the possibility of codissemination of wild-type and recombinant adenoviruses. Human Gene Therapy 6:711–721
     [Google Scholar]
  16. Imler J. L., Chartier C., Dreyer D., Dieterle A., Sainte-Marie M., Faure T., Pavirani A., Mehtali M. 1996; Novel complementation cell lines derived from human lung carcinoma A549 cells support the growth of E1- deleted adenovirus vectors. Gene Therapy 3:75–84
     [Google Scholar]
  17. Khatri A., Xu Z. Z., Both G. W. 1997; Gene expression by atypical recombinant ovine adenovirus vectors during abortive infection of human and animal cells in vitro. Virology 239:226–237
     [Google Scholar]
  18. Kimelman D., Miller J. S., Porter D., Roberts B. E. 1985; E1a regions of the human adenoviruses and of the highly oncogenic simian adenovirus 7 are closely related. Journal of Virology 53:399–409
     [Google Scholar]
  19. Klonjkowski B., Gilardi-Hebenstreit P., Hadchouel J., Randrianarison V., Boutin S., Yeh P., Perricaudet M., Kremer E. J. 1997; A recombinant E1- deleted canine adenoviral vector capable of transduction and expression of a transgene in human-derived cells and in vivo. Human Gene Therapy 8:2103–2115
     [Google Scholar]
  20. Lowe S. W., Ruley H. E. 1993; Stabilization of the p53 tumor suppressor is induced by adenovirus-5 E1A and accompanied apoptosis. Genes & Development 7:535–545
     [Google Scholar]
  21. Mittal S. K., Prevec L., Graham F. L., Babiuk L. A. 1995; Development of a bovine adenovirus type 3-based expression vector. Journal of General Virology 76:93–102
     [Google Scholar]
  22. Prevec L., Schneider M., Rosenthal K. L., Belbeck L. W., Derbyshire J. B., Graham F. L. 1989; Use of human adenovirus-based vectors for antigen expression in animals. Journal of General Virology 70:429–434
     [Google Scholar]
  23. Rao L., Debbas M., Sabbatini P., Hockenberry D., Korsmeyer S., White E. 1992; The adenovirus E1A protein induces apoptosis which is inhibited by the E1B 19k and bcl-2 proteins. Proceedings of the National Academy of Sciences USA 89:7742–7746
     [Google Scholar]
  24. Reddy P. S., Idamakanti N., Song J. Y., Lee J. B., Hyun B. H., Park J. H., Cha S. H., Tikoo S. K., Babiuk L. A. 1998a; Sequence and transcription map analysis of early region-1 of porcine adenovirus type- 3. Virus Research 58:97–106
     [Google Scholar]
  25. Reddy P. S., Idamakanti N., Song J. Y., Lee J. B., Hyun B. H., Park J. H., Cha S. H., Bae Y. T., Tikoo S. K., Babiuk L. A. 1998b; Nucleotide sequence and transcription map of porcine adenovirus type 3. Virology 251:414–426
     [Google Scholar]
  26. Reddy P. S., Idamakanti N., Hyun B.-H., Tikoo S. K., Babiuk L. A. 1999; Development of porcine adenovirus- 3 as an expression vector. Journal of General Virology 80:563–570
     [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  28. Sheppard M., Werner W., Tsatas E., McCoy R., Prowse S., Johnson M. 1998; Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease. Archives of Virology 143:915–930
     [Google Scholar]
  29. Tooze J. 1981 The Molecular Biology of Tumor Viruses, 2nd edn. part 2, DNA Tumor Viruses Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  30. Van den Elsen P., Houweling A., Van der Eb A. 1983; Expression of region E1b of human adenoviruses in the absence of region E1a is not sufficient for complete transformation. Virology 128:377–390
     [Google Scholar]
  31. Xiang Z. Q., Yang Y., Wilson J. M., Ertl H. C. J. 1996; A replication-defective human adenovirus recombinant serves as a highly efficacious vaccine carrier. Virology 219:220–227
     [Google Scholar]
  32. Xu Z. Z., Hyatt A., Boyle D. B., Both G. W. 1997; Construction of ovine adenovirus recombinants by gene insertion or deletion of related terminal region sequences. Virology 230:62–71
     [Google Scholar]
  33. Zakhartchouk A. N., Reddy P. S., Baxi M. K., Baca-Estrada M. E., Mehtali M., Babiuk L. A., Tikoo S. K. 1998; Construction and characterization of E3-deleted bovine adenovirus type 3 expressing full- length and truncated form of bovine herpesvirus type 1 glycoprotein gD. Virology 250:220–229
     [Google Scholar]
  34. Zakhartchouk A. N., Pyne C., Mutwiri G. K., Papp Z., Baca-Estrada M. E., Griebel P., Babiuk L. A., Tikoo S. K. 1999; Mucosal immunization of calves with recombinant bovine adenovirus-3: induction of protective immunity to bovine herpesvirus-1. Journal of General Virology 80:1263–1269
     [Google Scholar]
  35. Zheng B., Mittal S. K., Graham F. L., Prevec L. 1994; The E1 sequence of bovine adenovirus type 3 and complementation of human adenovirus type E1A function in bovine cells. Virus Research 31:163–186
     [Google Scholar]
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Porcine adenovirus-3 as a helper-dependent expression vector
J. Gen. Virol. 80, 2909 (1999); https://doi.org/10.1099/0022-1317-80-11-2909
/content/journal/jgv/10.1099/0022-1317-80-11-2909
/content/journal/jgv/10.1099/0022-1317-80-11-2909
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